We narrowed to 7,329 results for: GAN

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pSEM390 - mosTI unc-119 - Peft-3 - NNN - tbb-2

Plasmid

#182350

Purpose

Ubiquitous expression cloning vector for mosTI(unc-119). Transgene inserted between Peft-3 and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)

Depositor

Christian Froekjaer-Jensen

Publication

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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pSEM391 - mosTI unc-119 - Pmex-5 - NNN - tbb-2

Plasmid

#182351

Purpose

Germline expression cloning vector for mosTI(unc-119). Transgene inserted between Pmex-5 and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)

Depositor

Christian Froekjaer-Jensen

Publication

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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TVBB C-term-mTurquoise2

Plasmid

#169223

Purpose

Targeting vector backbone to support a knock-in of Linker-mTurquoise2 at the C-terminus of a target locus

Depositor

Hugo Snippert

Publication

Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

Insert

Double SapI flanked mTurquoise2

Use

Targeting vector backbone

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-MUTED

Plasmid

#164629

Purpose

Expresses MUTED in mammalian cells with a GFP tag

Depositor

Mark Cookson

Publication

Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

Insert

MUTED (BLOC1S5 Human)

Tags

cycle 3 GFP

Expression

Mammalian

Promoter

CMV

Available since

June 12, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_ESRRB_Hormone-recep

Plasmid

#109909

Purpose

Protein expression and purification of ESRRB_Hormone-recep

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

ESRRB_Hormone-recep (ESRRB Human)

Expression

Bacterial

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pNH13 (pmyo-2::QuasAr::mOrange)

Plasmid

#130273

Purpose

Expresses the eFRET-based voltage sensor QuasAr::mOrange in the pharynx of C. elegans.

Depositor

Alexander Gottschalk

Publication

Azimi Hashemi et al Proc Natl Acad Sci U S A. 2019 Aug 1. pi

Insert

QuasAr::mOrange

Tags

mOrange

Expression

Worm

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ER-rsTagRFP

Plasmid

#87620

Purpose

Endoplasmic reticulum (ER)-targeted rsTagRFP.

Depositor

Jin Zhang

Publication

Hertel et al Cell Rep. 2016 Jan 12;14(2):390-400. doi

Insert

ER-rsTagRFP

Tags

N-terminal 27 amino acids from CYP450

Expression

Mammalian

Promoter

CMV

Available since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pPB-R1R2_EF1ap300dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

Plasmid

#113343

Purpose

Expresses p300-dCas9-VP64 and MS2-p65-HSF1 fusion proteins

Depositor

Gavin Wright

Publication

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

p300core-dCas9-VP64-T2A-MS2-p65-HSF1

Use

Piggybac

Available since

Dec. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pSEM232 - [Pmlc-1 | tagRFP-T (2x NLS)| cbr-tbb-2 UTR]

Plasmid

#159898

Purpose

Fluorescent co-injection marker to identify extrachromosomal arrays in C. elegans

Depositor

Christian Froekjaer-Jensen

Publication

El Mouridi et al MicroPubl Biol. 2020 Sep 3;2020. doi: 10

Insert

Pmlc-1 | tagRFP-T (2x NLS)| cbr-tbb-2 UTR

Expression

Worm

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_CAPNS1_EF-hand_8+EF-hand_5

Plasmid

#109995

Purpose

Protein expression and purification of CAPNS1_EF-hand_8+EF-hand_5

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

CAPNS1_EF-hand_8+EF-hand_5 (CAPNS1 Human)

Expression

Bacterial

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pLSU4cg10

Plasmid

#126078

Purpose

Plant transformation vector for stable expression of plastid targeted GFP1-10 in plant cells

Depositor

Ralf-Bernd Klösgen

Publication

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

Chloroplastic FNR transit peptide

Use

Binary

Tags

GFP1-10

Expression

Plant

Promoter

longCaMV35S

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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miniSOG-Clathrin-15

Plasmid

#57764

Purpose

Localization: Clathrin Vesicles, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Publication

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

Clathrin (CLTB Human)

Tags

miniSOG

Expression

Mammalian

Promoter

CMV

Available since

Jan. 29, 2015

Availability

Industry, Academic Institutions, and Nonprofits
2xHA MED10 pBacPAK8

Plasmid

#17321

Depositor

Joan Conaway, Ronald Conaway

Publication

Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

Insert

MED10 (Med10 Mouse)

Tags

2xHA

Expression

Insect

Available since

March 11, 2008

Availability

Academic Institutions and Nonprofits only
pLJM1-PHD2

Plasmid

#223552

Purpose

Lentivirus transfer plasmid for expression of full length human PHD2

Depositor

Somshuvra Mukhopadhyay

Publication

Gurol et al Proc Natl Acad Sci U S A. 2024 Jun 25;12

Insert

PHD2 (EGLN1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available since

Sept. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pML531_TOMM20-C-term_XTEN80-mEGFP-3xHA_pTMPLT

Plasmid

#227890

Purpose

Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.

Depositor

Manuel Leonetti

Publication

Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.

Insert

TOMM20 homology arms with mEGFP-3xHA for insertion at C terminus

Use

CRISPR

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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N-Flag-IPO9_Lenti SFFV Puro WPRE

Plasmid

#217916

Purpose

Express IPO9 in mammalian cells

Depositor

Benjamin Cravatt

Publication

Ogasawara et al Cell Chem Biol. 2024 Nov 7:S2451-9456(24

Insert

importin 9 (IPO9 Human)

Tags

Flag

Expression

Mammalian

Available since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-FAB(PX-LD)

Plasmid

#214422

Purpose

Detection of peroxisome-lipid droplet contact site

Depositor

Chi-Lun Chang

Publication

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

PMP34-FKBP-V5-RspAN_IRES_CFAST-20nm-Halo-6xHp NC (SLC25A17 Human)

Use

Lentiviral

Tags

CFAST-Halo and FKBP-V5-RspAN

Promoter

CMV

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pQE60 R130A wgMDH

Plasmid

#204266

Purpose

Bacterial expression of R130A wgMDH with IPTG induction - Active site mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Publication

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Tags

6X His

Expression

Bacterial

Mutation

R130A

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCEP4-MR1

Plasmid

#178603

Purpose

Mammalian expression of myc-tagged MR1

Depositor

Erik Procko

Publication

McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

Insert

MR1 (MR1 Human)

Tags

Extracellular c-myc epitope ta and Signal peptide…

Expression

Mammalian

Promoter

CMV

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pALS343 (pGEX-2TP-cTevHis-Gibson/MCS)

Plasmid

#139788

Purpose

Vector for recombinant protein expression in E. coli; PreScission-cleavable N-terminal GST and TEV-cleavable C-terminal 6xHis

Depositor

Arneet Saltzman

Publication

Saltzman et al Genetics. 2018 Nov;210(3):907-923. doi:

Type

Empty backbone

Tags

6xHis (cleavable by TEV protease) and GST (cleava…

Expression

Bacterial

Available since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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