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pENTR-R4R3-mEGFP-N

Plasmid

#113746

Purpose

Gateway multi-site entry clone for second position mEGFP (N-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

mEGFP

Use

CRISPR; Gateway entry vector

Tags

mEGFP

Expression

Mutation

Promoter

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-R1ACR_653-EYFP

Plasmid

#111079

Purpose

Anion channelrhodopsin from Rhodomonas sp. for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

Insert

Anion channelrhodopsin R1ACR_653

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

Available since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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pRKVSV FRNK

Plasmid

#50534

Depositor

Kenneth Yamada

Article

Segarra et al FASEB J. 2005 Nov;19(13):1875-7.

Insert

protein tyrosine kinase, domain

Use

Tags

2X VSV

Expression

Mammalian

Mutation

truncated (NT2186-3268)

Promoter

CMV

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pENTR-R4R3-mEGFP-C

Plasmid

#113749

Purpose

Gateway multi-site entry clone for second position mEGFP (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

mEGFP

Use

CRISPR; Gateway entry vector

Tags

mEGFP

Expression

Mutation

Promoter

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-R4R3-mRuby-C

Plasmid

#113755

Purpose

Gateway multi-site entry clone for second position mRuby (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

mRuby2

Use

CRISPR; Gateway entry vector

Tags

mRuby2

Expression

Mutation

Promoter

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR-R4R3-3xmRuby-C

Plasmid

#113757

Purpose

Gateway multi-site entry clone for second position 3x mRuby (C-terminal tag). Introduced into the genome via homology-directed repair after an LR reaction with homology sequences.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

mRuby2-mRuby2-mRuby2

Use

CRISPR; Gateway entry vector

Tags

3xmRuby2

Expression

Mutation

Promoter

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pED45: pRS306 pRPS2-Ste7DS-NLS-mCherry

Plasmid

#68274

Purpose

SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS mCherry

Depositor

Serge Pelet

Article

Durandau et al BMC Biol. 2015 Aug 1;13:55. doi: 10.1186

Insert

Ste7DS NLS mCherry

Use

Tags

mCherry

Expression

Yeast

Mutation

Promoter

pRPS2

Available since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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mCherry-miniSOG-Display-5

Plasmid

#54802

Purpose

Localization: Cytoplasm, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Type

Empty backbone

Use

Tags

mCherry-miniSOG-Display

Expression

Mammalian

Mutation

Promoter

Available since

July 10, 2014

Availability

Academic Institutions and Nonprofits only
RCAS (A) Caronte (CT#382)

Plasmid

#13862

Depositor

Cliff Tabin

Article

Yokouchi et al Cell. 1999 Sep 3. 98(5):573-83.

Insert

Caronte (CER1 Chicken)

Use

Retroviral; Avian expression

Tags

Expression

Mutation

Promoter

Available since

Feb. 9, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-Nup88 (551-741)

Plasmid

#64252

Purpose

Mammalian expression of C-terminal domain of Nup88

Depositor

Birthe Fahrenkrog

Article

Lussi et al Mol Biol Cell. 2011 Apr;22(7):1080-90. d

Insert

Nup88 (NUP88 Human)

Use

Tags

his and myc

Expression

Mammalian

Mutation

C-terminal domain (551-741)

Promoter

CMV

Available since

April 23, 2015

Availability

Academic Institutions and Nonprofits only
pRKVSV FAT

Plasmid

#50533

Depositor

Kenneth Yamada

Article

Segarra et al FASEB J. 2005 Nov;19(13):1875-7.

Insert

protein tyrosine kinase, domain

Use

Tags

2X VSV

Expression

Mammalian

Mutation

truncated (NT2630-3268)

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pR-Avr

Plasmid

#22650

Depositor

Lynn Hudson

Article

Kim et al J Neurosci Res. 1997 Oct 15. 50(2):272-9

Insert

Myelin Transcription Factor 1 (Myt1 Rat)

Use

Cloning and rna synthesis

Tags

Expression

Mutation

Promoter

Available since

Dec. 1, 2009

Availability

Academic Institutions and Nonprofits only
pmMyt1

Plasmid

#22713

Depositor

Lynn Hudson

Article

Kim et al J Neurosci Res. 1997 Oct 15. 50(2):272-9

Insert

Myelin Transcription Factor 1 (Myt1 Mouse)

Use

Cloning vector tt/aa

Tags

Expression

Mutation

Promoter

Available since

Dec. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pCaSpeR-hs-taiman

Plasmid

#17585

Depositor

Denise Montell

Article

Bai et al Cell. 2000 Dec 22. 103(7):1047-58.

Insert

taiman (tai Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only
pAAV-pCAG-FRT(F3F5)-ChRmine3.3-p2A-oScarlet,rev.-WPRE

Plasmid

#234895

Purpose

CAG-driven, Flp-dependent ChRmine for optogenetic excitation of neurons with an oScarlet tag

Depositor

Silvia Arber

Article

Falasconi et al Nature. 2025 May 28. doi: 10.1038/s41586

Insert

FRT(F3F5)-ChRmine3.3-p2A-oScarlet

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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pk335-BFP

Plasmid

#227505

Purpose

Backbone for CARGO cloning, with U6 promoter and gRNA scaffold, TagBFP, and Kanamycin resistence

Depositor

Joanna Wysocka

Article

Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 5, 2025

Availability

Academic Institutions and Nonprofits only

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pGEL1022

Plasmid

#225593

Purpose

The backbone vector for rice genome editing is based on HkCas12a

Depositor

Yong Zhang

Article

Liu et al Plant Biotechnol J. 2024 Oct 10. doi: 10

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEL1023

Plasmid

#225594

Purpose

The backbone vector for rice genome editing is based on PrCas12a

Depositor

Yong Zhang

Article

Liu et al Plant Biotechnol J. 2024 Oct 10. doi: 10

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEL1024

Plasmid

#225595

Purpose

The Dual Pol II backbone vector for rice genome editing is based on Mb3Cas12a

Depositor

Yong Zhang

Article

Liu et al Plant Biotechnol J. 2024 Oct 10. doi: 10

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEL1025

Plasmid

#225596

Purpose

The STU backbone vector for rice genome editing is based on Mb3Cas12a

Depositor

Yong Zhang

Article

Liu et al Plant Biotechnol J. 2024 Oct 10. doi: 10

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge