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iRFP670-PIP4K2C

Plasmid

#202750

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Publication

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

iRFP670:PIP4K2C (PIP4K2C Human)

Expression

Mammalian

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRU293

Plasmid

#167688

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Publication

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pEC1.2::Cas9i

Use

CRISPR

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pRU54

Plasmid

#167680

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Publication

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pEC1.2::spCas9

Use

CRISPR

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pBTSG

Plasmid

#159420

Purpose

Transient expression of proteins with a C-terminal thermostable GFP (TGP) tag in mammalian cells

Depositor

Dianfan Li

Publication

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

thermostable GFP (TGP)

Tags

polyhistidine (poly-His)

Expression

Mammalian

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pHJ8: CadC-VHH-Control

Plasmid

#108243

Purpose

membrane bound transcription activator CadC fusing with anti-RNase A antibody

Depositor

Jerome Bonnet

Publication

Chang et al ACS Synth Biol. 2017 Oct 30. doi: 10.102

Insert

Membrane bound transcription activator CadC fusing with anti-RNase A VHH antibody

Use

Synthetic Biology

Promoter

pLacO1

Available since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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PDZD7-TwinStrepII

Plasmid

#83198

Purpose

Expression of tagged protein in mammalian cells

Depositor

Peter Barr-Gillespie

Publication

Morgan et al Elife. 2016 Aug 15;5:e18312. doi: 10.755

Insert

PDZD7 (Pdzd7 Mouse)

Tags

Twin Strep Tag II

Expression

Mammalian

Promoter

CMV

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pDelila (GB0079)

Plasmid

#68200

Purpose

Provides the CDS of the Antirrhinum majus Delila transcription factor as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Delila coding region

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDsRed (GB0100)

Plasmid

#68202

Purpose

Provides the CDS of DsRed as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

DsRed coding region

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pHHSDKXK (#19)

Plasmid

#35964

Depositor

Thomas Sato

Publication

Schlaeger et al Proc Natl Acad Sci U S A. 1997 Apr 1;94(

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

Tie2 promoter
SDKLacZ
SV40 pA
Tie2 enhancer

Available since

July 9, 2013

Availability

Academic Institutions and Nonprofits only
TAL2314

Plasmid

#36740

Depositor

Keith Joung

Publication

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

FANCF-TALEN_Left (Fancf Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available since

July 18, 2012

Availability

Academic Institutions and Nonprofits only
pGWB405-TIC21-sGFP

Plasmid

#213865

Purpose

Binary vector for the expression of sGFP flanked with an N-terminal chloroplas inner envelope membrane transit peptide in plants .

Depositor

Yutaka Kodama

Publication

Ichikawa et al Plant Direct. 2022 Nov 15;6(11):e462. do

Insert

chloroplast inner envelope membrane transit peptide (TIC21 Mustard Weed)

Expression

Bacterial and Plant

Available since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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STAgR_tdTomato

Plasmid

#102993

Purpose

Can be used as backbone for a STAgR reaction

Depositor

Stefan Stricker

Publication

Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

Type

Empty backbone

Use

CRISPR; Pcr template for stagr vectors

Promoter

U6

Available since

March 13, 2019

Availability

Academic Institutions and Nonprofits only

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pFastBac GST-Plk4

Plasmid

#80264

Purpose

baculovirus creation plasmid for GST-Plk4

Depositor

Andrew Holland

Publication

Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

Insert

Plk4 (PLK4 Human)

Tags

GST

Expression

Insect

Available since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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pRosea (GB0026)

Plasmid

#68194

Purpose

Provides the CDS of the Antirrhinum majus Rosea transcription factor as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Publication

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

A. majus Rosea coding region

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available since

Oct. 26, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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TAL2417

Plasmid

#36818

Depositor

Keith Joung

Publication

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

FANCA-TALEN_Right (Fanca Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available since

July 13, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
TAL2254

Plasmid

#36697

Depositor

Keith Joung

Publication

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

FANCC-TALEN_Left (Fancc Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
NpuDnaEc-cGCaMP2

Plasmid

#34851

Depositor

Kevin Truong

Publication

Wong et al Cell Calcium. 2011 Nov 29.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NpuDnaEc-cGCaMP2

Expression

Bacterial and Mammalian

Promoter

CMV, T7 lac

Available since

June 21, 2012

Availability

Academic Institutions and Nonprofits only
GFP-NHERF1 WT

Plasmid

#195213

Purpose

Mammalian expression vector for GFP tagged NHERF1.

Depositor

Anthony Bretscher, David Bryant

Publication

Garbett et al J Cell Biol. 2010 Oct 18;191(2):397-413.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NHERF1 (NHERF1 Human)

Expression

Mammalian

Available since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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pGEMHE-mScarlet-MAP4-MTBD

Plasmid

#171488

Purpose

T7 promotor drives in vitro transcription of mScarlet-tagged mouse MAP4-Microtubule Binding Domain mRNA

Depositor

Melina Schuh

Publication

Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

Insert

MAP4-MTBD (Map4 Mouse)

Use

Pgehme

Tags

mScarlet

Mutation

Aminoacids 658-1126

Promoter

T7

Available since

July 15, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2+FucciOrange

Plasmid

#128427

Purpose

Contains insert allowing in-vivo visualization of Cdk1 protein (G1)

Depositor

Rusty Lansford

Publication

Tran et al J Vis Exp. 2019 Jun 7;(148). doi: 10.379

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

FucciOrange

Expression

Mammalian

Promoter

CMV & SP6

Available since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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