We narrowed to 3,800 results for: golden gate

1. pEPOR0SP0017

   Plasmid

   #117527

   Purpose

   Level0 Golden Gate part: CDS, Sp-eCas9 1.0 (no stop codon)

   Depositor

   Nicola Patron

   Article

   Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

   Insert

   Sp-eCas9 1.0 (no stop codon)
   

   Use

   CRISPR

   Mutation

   K810A, K1003A, R1060A

   Available Since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTU2_2b

   Plasmid

   #73011

   Purpose

   Secondary module (BpiI) site in pTU2b backbone

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   Secondary module + Golden Gate pTU2b BsmBI destination site
   

   Use

   Synthetic Biology

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIF1079

   Plasmid

   #237445

   Purpose

   Expression SpCas9 and Efe1-RT from bidirectional CAG promoters. SpCas9 sgRNA and Efe1 non-coding expression is driven by U6 and H1 respecitvely.

   Depositor

   IIya Finkelstein

   Article

   Buffington et al bioRxiv 2024.07.21.604473; doi: 10.1101/

   Inserts

   SpCas9
   Efe1-RT
   EMX1 sgRNA
   Efe1-ncRNA targeting EMX1
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CAG, H1, and U6

   Available Since

   June 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNP2322 [Cloning backbone, mCherry Inversion Activity Reporter]

   Plasmid

   #247250

   Purpose

   Cloning backbone for measuring bridge recombinase mediated inversion

   Depositor

   Patrick Hsu

   Article

   Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Oct. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNP566

   Plasmid

   #222724

   Purpose

   pNP561 - IS621 WT Target with T7 Inducible IS621 Recombinase

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

   Insert

   IS621 Recombinase
   

   Expression

   Bacterial

   Available Since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. EF1a-mGL(miRE.FF4)-bGH

   Plasmid

   #235264

   Purpose

   microRNA expression for dual-vector iFFL circuit

   Depositor

   Kate Galloway

   Article

   Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

   Insert

   mGreenLantern
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   EF1a (human EF1a)

   Available Since

   May 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCB094 FoMV-sgLw

   Plasmid

   #234816

   Purpose

   To express guide gRNA from Foxtail Mosaic Virus Vector targeting SbLw (T-DNA)

   Depositor

   Daniel Voytas

   Article

   Baysal et al Plant J. 2025 Jan;121(2):e17196. doi: 10

   Insert

   gRNA targeting SbLw
   

   Expression

   Plant

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHelper_ShCAST_sgRNA

   Plasmid

   #127921

   Purpose

   Expresses ShCAST and an empty sgRNA scaffold. New targets can be added using Golden Gate assembly (LguI sites).

   Depositor

   Feng Zhang

   Article

   Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

   Insert

   ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning (LguI sites)
   

   Available Since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCEC-red

   Plasmid

   #196040

   Purpose

   Allows the expression of Cas9 in Saccharomyces cerevisiae and contains a gRNA expression unit with a mRFP1 placeholder for user-defined gRNA insertion using BsaI-mediated Golden Gate Assembly

   Depositor

   Paola Branduardi

   Article

   Maestroni et al FEMS Yeast Res. 2023 Jan 4;23:foad002. d

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology; Grna cloning

   Expression

   Yeast

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIVT-p53DD

    Plasmid

    #232751

    Purpose

    IVT template for making modRNA encoding p53DD, a mutant p53 that lacks DNA binding ability

    Depositor

    Kate Galloway

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    p53DD
    

    Use

    In vitro transcription

    Mutation

    deletion of DNA binding domain

    Available Since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCB092 FoMV-sgMgCh

    Plasmid

    #234815

    Purpose

    To express guide gRNA from Foxtail Mosaic Virus Vector targeting SbMgCh (T-DNA)

    Depositor

    Daniel Voytas

    Article

    Baysal et al Plant J. 2025 Jan;121(2):e17196. doi: 10

    Insert

    gRNA targeting SbMgCh
    

    Expression

    Plant

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. TL1 DdCBE A1 (DddA6-C)

    Plasmid

    #221992

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-T-TL1 TALE A1 repeats-TALE CTD-DddA6/11 1397C-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    DddA: T1413I

    Promoter

    CMV

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. TL1 DdCBE T2 (DddA6-N)

    Plasmid

    #221993

    Purpose

    Expresses mitochondrial cytosine base editor arm in mammalian cells

    Depositor

    Stephen Ekker

    Article

    Castillo et al Hum Gene Ther. 2024 Sep 24. doi: 10.1089

    Insert

    COX8A MTS-3xFLAG-TALE NT-T-TL1 TALE T2 repeats-TALE CTD-DddA6 1397N-UGI-ATP5B 3'UTR
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    DddA: Q1310R, S1330I, T1380I

    Promoter

    CMV

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lenti-entry-puro

    Plasmid

    #85745

    Purpose

    Lentiviral vector for generating multiple sgRNAs carrying plasmids by Golden Gate Assembly.

    Depositor

    Qin Yan

    Article

    Cao et al Nucleic Acids Res. 2016 Nov 2;44(19):e14

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. L0-I35 p35S-XVE-pEstradiol GG0-GG1

    Plasmid

    #195904

    Purpose

    Level 0 inducibile estradiol promter and XVE activator protein (p35S-XVE gene-tRBCS-tNOS-LexA-p35S with GG0 and GG1 golden gate linkers)

    Depositor

    Jennifer Nemhauser

    Article

    Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

    Insert

    p35S-XVE-LexA-p35S
    

    Use

    Synthetic Biology

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pShHELIX_sgRNA_entry (CJT32)

    Plasmid

    #181782

    Purpose

    Expresses ShHELIX containing a nicking I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    nAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
    

    Expression

    Bacterial

    Mutation

    nAniI = K227M, F80K, L232K

    Promoter

    Lac and J23119

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRAM28

    Plasmid

    #234314

    Purpose

    pColE1 origin Cat-RNA U1376, Multiplexing ribozyme splicing reactions for the purpose of comparing host range of origins of replications

    Depositor

    James Chappell

    Article

    Kalvapalle et al Nat Biotechnol. 2025 Mar 18. doi: 10.103

    Insert

    U1367 Targeting RAM, Barcode 2
    

    Use

    Synthetic Biology

    Promoter

    P. CymRC

    Available Since

    March 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAGM4723:TpCC_Urease

    Plasmid

    #85982

    Purpose

    Golden Gate Level 2 construct encoding Cas9-YFP and 2 urease-targeting gRNAs

    Depositor

    Thomas Mock

    Article

    Hopes et al Plant Methods. 2016 Nov 24;12:49. doi: 1

    Inserts

    Cas9
    Urease-targeting gRNA 1
    Urease-targeting gRNA 2
    

    Use

    CRISPR; Diatom (t. pseudonana)

    Tags

    NLS and YFP

    Promoter

    FCP and U6

    Available Since

    Jan. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pC-GoldyTALEN

    Plasmid

    #38143

    Purpose

    destination vector for the Golden Gate TALEN kit, directs expression of TALENs from a truncated CAGs promoter

    Depositor

    Daniel Carlson

    Article

    Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 1.

    Insert

    GoldyTALEN
    

    Tags

    AcV5 and FokI homodimer

    Expression

    Mammalian

    Mutation

    Truncate at N and C terminous

    Promoter

    miniCaggs

    Available Since

    July 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNP1830

    Plasmid

    #222728

    Purpose

    pNP561 - T5 Target with T7 Inducible IS621 Recombinase

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

    Insert

    IS621 Recombinase
    

    Expression

    Bacterial

    Available Since

    July 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge