We narrowed to 4,933 results for: puc

1. phU1-PspCas13b-non-targeting (pEZ36)

   Plasmid

   #229053

   Purpose

   Cloning backbone for PspCas13b crRNA (Human U1 promoter)

   Depositor

   S Chul Kwon

   Article

   Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAX1

   Plasmid

   #117397

   Purpose

   allelic exchange vector with GFP merodiploid tracker and temperature-sensitive origin of replication

   Depositor

   Karen Guillemin

   Article

   Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmU1-CasRx-non-targeting (pKW83)

   Plasmid

   #229051

   Purpose

   Cloning backbone for CasRx crRNA (Mouse U1 promoter)

   Depositor

   S Chul Kwon

   Article

   Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pG106 - KA

   Plasmid

   #178041

   Purpose

   Bacteroides - Escherichia coli shuttle vector with ErmF and ErmAM, Kan and Amp selection markers

   Depositor

   Janina Lewis

   Article

   Jones et al Mol Oral Microbiol. 2020 Aug;35(4):181-1

   Type

   Empty backbone

   Use

   Shuttle vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 1, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pSBbi-RN

   Plasmid

   #60519

   Purpose

   SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and neomycin resistance gene

   Depositor

   Eric Kowarz

   Article

   Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

   Type

   Empty backbone

   Use

   Transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a/RPBSA

   Available since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCHW00019

   Plasmid

   #222448

   Purpose

   Level 0, C terminal tag module (TTCG-GCTT) containing GAL4AD-NLS.

   Depositor

   Chih-Hang Wu

   Article

   Chiang et al bioRxiv 2024.03.23.586378

   Insert

   GAL4AD-NLS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   no

   Promoter

   Available since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. TC374

   Plasmid

   #164886

   Purpose

   Prpl28::rtetR-QFAD::p2A::mKate::T2A::tetR-pie1,

   Depositor

   Tian Chi

   Article

   Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

   Insert

   rpl28-rtTA(Q)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTO135_pENO1-CaCas9-tCyc1

   Plasmid

   #171103

   Purpose

   Expresses Cas9 in Candida auris

   Depositor

   Teresa O'Meara

   Article

   Santana et al Nat Commun. 2021 Dec 10;12(1):7197. doi:

   Insert

   CaCas9
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ENO1

   Available since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. AICSDP-82:SON-mEGFP

   Plasmid

   #133964

   Purpose

   Homology arms and mEGFP-linker sequence for N-terminus tagging of human SON

   Depositor

   Allen Institute for Cell Science

   Insert

   SON Homology Arms with mEGFP-linker (SON Human)
   

   Use

   CRISPR

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Promoter

   Available since

   Nov. 22, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. PB-hEF1a-MG

    Plasmid

    #175504

    Purpose

    All-in-one piggyBac transposon destination vector for hEF1a constitutive expression of Mega Gate cloned elements

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hEF1a

    Available since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKSJ331

    Plasmid

    #103063

    Purpose

    Expresses colicin E1 and E1 immunity protein without E1 lysis (kil) gene

    Depositor

    Alan Finkelstein, Karen Jakes

    Article

    Kienker et al J Gen Physiol. 2008 Dec;132(6):693-707.

    Insert

    Colicin E1 and E1 immunity protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    colicin promotor from ColE1

    Available since

    Dec. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSBbi-BN

    Plasmid

    #60518

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: BFP and neomycin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTn7-SCOUT11.B

    Plasmid

    #201005

    Purpose

    pLv1-pUC18R6KT-miniTn7-Gm-TagBFP, GmR, ApR

    Depositor

    Philip Poole

    Article

    Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

    Insert

    TagBFP
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23104

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pmCherry-GFP

    Plasmid

    #86441

    Purpose

    visualization and quantifying the out-of-frame mutations from genome editing

    Depositor

    Yun-Bo Shi

    Article

    Fu et al Sci Rep. 2016 Oct 17;6:35488. doi: 10.10

    Insert

    mCherry-MCS-sfGFP fusion gene
    

    Use

    Color visualization in bacteria

    Tags

    mCherry-sfGFP

    Expression

    Mutation

    Promoter

    Plac

    Available since

    Nov. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. dg263_pENTR_ChrimsonCel

    Plasmid

    #66101

    Purpose

    Entry clone containing Chrimson coding sequence (without stop codon), codon optimized for C. elegans, with synthetic introns, flanked by attL1 and attL2

    Depositor

    Dominique Glauser

    Article

    Schild et al Genetics. 2015 May 28. pii: genetics.115

    Insert

    Chrimson
    

    Use

    Gateway

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. LI C-D GUS

    Plasmid

    #104517

    Purpose

    LI Gene of Interest module

    Depositor

    Thomas Lahaye

    Article

    Wu et al Mol Plant Pathol. 2017 Oct 27. doi: 10.1

    Insert

    UidA gene
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. TGT-His

    Plasmid

    #138201

    Purpose

    Expresses E. Coli TGT with a C-terminal His Tag

    Depositor

    Neal Devaraj

    Article

    Busby et al Methods Enzymol. 2020;641:373-399. doi:

    Insert

    E Coli tRNA Guanine Transglycosylase-His
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLacZa-GFP

    Plasmid

    #86440

    Purpose

    visualization and quantifying the out-of-frame mutations from genome editing

    Depositor

    Yun-Bo Shi

    Article

    Fu et al Sci Rep. 2016 Oct 17;6:35488. doi: 10.10

    Insert

    LacZalpha-MCS-sfGFP fusion gene
    

    Use

    Color visualization in bacteria

    Tags

    LacZa-sfGFP

    Expression

    Mutation

    Promoter

    Plac

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. ITPR1_Halo_N_allele

    Plasmid

    #178161

    Purpose

    Donor vector for endogenous tagging of human ITPR1 at the N-terminus with halotag

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Insert

    Halotag (ITPR1 Human)
    

    Use

    Donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. DNMT1_Halo_C_allele

    Plasmid

    #178157

    Purpose

    Donor vector for endogenous tagging of human DNMT1 at the C-terminus with halotag

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Insert

    Halotag (DNMT1 Human)
    

    Use

    Donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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