We narrowed to 31,019 results for: uros

1. pSicoR-Ef1a-mCh-Puro-GFPi

   Plasmid

   #31848

   Depositor

   Bruce Conklin

   Article

   Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

   Insert

   GFP RNAi
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 for shRNA; Ef1alpha for mCherry-puromycin

   Available since

   Jan. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDH-puro/Flag PD-L1 N35/3NQ

   Plasmid

   #121455

   Purpose

   expression of human PD-L1 mutant in mammalian cells

   Depositor

   Mien-Chie Hung

   Article

   Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

   Insert

   CD274 (CD274 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   N192Q, N200Q and N219Q

   Promoter

   CMV

   Available since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSUPER retro puro macroH2A2 shRNA

   Plasmid

   #30518

   Depositor

   John Gurdon

   Article

   Pasque et al EMBO J. 2011 May 6;30(12):2373-87. doi:

   Insert

   mH2A2 shRNA
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. qTAG-C-moxGFP-Puro-H2BC11-MMEJ

   Plasmid

   #207760

   Purpose

   MMEJ donor template for moxGFP-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H2BC11 Short Homology Arms flanking a moxGFP-Puro Cassette (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pCRISPaint-mNeon-F-hgp100-PuroR [M1G]

   Plasmid

   #171801

   Purpose

   Universal donor plasmid for CRISPitope method encoding mNeon fluorescent protein, FLAG tag, human gp100 CD8+ T cell epitope [AA: 25-33 (KVPRNQDWL)] and puromycin resistance cassette [p.M1G]

   Depositor

   Michael Hoelzel

   Article

   Effern et al Immunity. 2020 Sep 15;53(3):564-580.e9.

   Insert

   mNeonGreen-FLAG-hgp100-T2A-PuroR
   

   Use

   Gene tagging

   Tags

   Expression

   Mutation

   PuroR: Changed Methionin 1 to Glycine.

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. integrin beta6 Y774F pBabe puro

   Plasmid

   #13599

   Depositor

   Dean Sheppard

   Insert

   integrin beta 6 Y774F (ITGB6 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Y774F

   Promoter

   Available since

   Jan. 8, 2007

   Availability

   Academic Institutions and Nonprofits only

7. H446 EF1a RA-ABE6.3 p2A Puro

   Plasmid

   #139104

   Purpose

   Lentiviral expression of ABE6.3.

   Depositor

   Wen Xue

   Article

   Song et al Nat Biomed Eng. 2019 Feb 25. pii: 10.103

   Insert

   ABE RA6.3
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL3-U6-sgRNA-PGK-puromycin-AflII

   Plasmid

   #106404

   Purpose

   Empty guide RNA cloning vector with an AflII site added from Addgene 41824

   Depositor

   Jason Gertz

   Article

   Carleton et al Cell Syst. 2017 Oct 25;5(4):333-344.e5.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBABE-2xFLAG-2xSTREP-CCND3-Puro

   Plasmid

   #172622

   Purpose

   Retroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D3 and a puromycin resistance marker in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND3 (CCND3 Human)
   

   Use

   Retroviral

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBabe-TetCMV-puro-mCerulean-PA-Rac1

    Plasmid

    #22031

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    mCerulean-PA-Rac1 (RAC1 Human, Avena sativa (oat))
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Rac1 starts at I4 and contains mutations Q61L, E9…

    Promoter

    Available since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

11. pBABE-2xFLAG-2xSTREP-CCND2-Puro

    Plasmid

    #172628

    Purpose

    Retroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D2 and a puromycin resistance marker in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CCND2 (CCND2 Human)
    

    Use

    Retroviral

    Tags

    2xFLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. Ef1a-somBiPOLES-mCerulean-IRES-Puro

    Plasmid

    #192585

    Purpose

    lentiviral vector for bidirectional optogenetic manipulation of Cre-positive neurons

    Depositor

    Simon Wiegert

    Insert

    somBiPOLES
    

    Use

    Lentiviral

    Tags

    soma-targeting motif from Kv2.1 channel

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1-alpha

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYJ21-lenti-EF1a-ASAP2f-puro

    Plasmid

    #131065

    Purpose

    Forced expression of ASAP2f

    Depositor

    Xiaojun Lian

    Article

    Jiang et al Stem Cell Res Ther. 2021 May 29;12(1):30

    Insert

    ASAP2f
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO-Tet-puro-hARAF-shRNA-1

    Plasmid

    #185370

    Purpose

    For tetracycline-inducible mammalian expression of shRNA: GCCGTGACCAGATTATCTTTA that targets human ARAF

    Depositor

    Ayaz Najafov

    Insert

    ARAF (ARAF Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLEX-MAPKAP1.1-FHH-IRES-Puro

    Plasmid

    #120705

    Purpose

    Expresses MAPKAP1.1-Flag-HA-6xHIS fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    MAPKAP1 Isoform 1
    

    Use

    Lentiviral

    Tags

    Flag-HA-6xHIS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. LV UAS-DsRed-Express2-PGK-Puro

    Plasmid

    #190801

    Purpose

    LV construct encoding UAS-DsRed-Express2 reporter gene.

    Depositor

    John Ngo

    Article

    Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

    Insert

    UAS-T2A-DsRed-Express2
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLKO.1-Tet-Puro-shMMERVK10c_1 (inducible)

    Plasmid

    #189955

    Purpose

    shRNA mediated knockdown

    Depositor

    Denes Hnisz

    Article

    Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

    Insert

    ERV_MMERVK10c
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. 1120 pBabe puroL HA FKHR H215R

    Plasmid

    #9026

    Depositor

    William Sellers

    Article

    Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

    Insert

    FKHR H215R (FOXO1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    H215R

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pPuro3.1(+)_Strep-Lrrk2(delGTPase)-Myc

    Plasmid

    #161587

    Purpose

    expression of Myc-tagged mouse Lrrk2 without GTPase domain

    Depositor

    Maik Hintze

    Insert

    Lrrk2 (Lrrk2 Mouse)
    

    Use

    Tags

    Myc tag and StrepII

    Expression

    Mammalian

    Mutation

    deletion: AA1023-1879

    Promoter

    CMV

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPuro3.1(+)_Strep-Lrrk2(delKinase)-Myc

    Plasmid

    #161586

    Purpose

    expression of Myc-tagged mouse Lrrk2 without kinase domain

    Depositor

    Maik Hintze

    Insert

    Lrrk2 (Lrrk2 Mouse)
    

    Use

    Tags

    Myc tag and StrepII

    Expression

    Mammalian

    Mutation

    deletion: AA1871-2540

    Promoter

    CMV

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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