We narrowed to 2,365 results for: DAP

1. qTAG-N-Solo-mStayGold-TJP1

   Plasmid

   #227300

   Purpose

   Donor template for mStayGold insertion into the N-terminus of the TJP1 locus. For tight junction visualization. To be co-transfected with sgRNA plasmid px330-TJP1 (Addgene #227299)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   TJP1 Homology Arms flanking a mStayGold Tag (TJP1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available Since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330-CENPA

   Plasmid

   #227282

   Purpose

   Expresses SpCas9 and a sgRNA targeting the N-terminus of CENPA for knock-in.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   sgRNA Targeting N-terminus of CENPA (CENPA Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Nov. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. qTAG-AAVS1-Ef1a-Puro-mScarlet

   Plasmid

   #227274

   Purpose

   AAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing mScarlet. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   AAVS1 Homology Arms flanking a 2A-Puro-EF1a-mScarlet cassette (AAVS1 Synthetic)
   

   Use

   CRISPR; Donor template

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Promoterless

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pmiRGLO Dual-Luciferase-HNRNPAB-3'UTR

   Plasmid

   #182249

   Purpose

   Human HNRNPAB 3` UTR region containing 2x binding sites for miR-150 cloned downstream of luciferase reporter gene

   Depositor

   Silvia Monticelli

   Article

   Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

   Insert

   3' UTR region of HNRNPAB gene
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3 myc card9

   Plasmid

   #16253

   Depositor

   William Kaelin

   Article

   Yang et al Mol Cell. 2007 Oct 12. 28(1):15-27.

   Insert

   card9 (CARD9 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 21, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pdCas12a(RuvC)

   Plasmid

   #186447

   Purpose

   Encodes catalytically inactive Cas12a (D917A, E1006A) from F. novicida (type V-A)

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Insert

   dCas12a
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D917A, E1006A

   Promoter

   T7

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pdCas12b(RuvC)

   Plasmid

   #186450

   Purpose

   Encodes catalytically inactive Cas12b (E848A, D977A) from A. acidoterrestris (type V-B)

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Insert

   dCas12b
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   E848A, D977A

   Promoter

   T7

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. psigma1-EGFP

   Plasmid

   #53611

   Purpose

   Expresses sigma1 of adaptor protein of AP1

   Depositor

   Tom Kirchhausen

   Article

   Kural et al Cell Rep. 2012 Nov 29;2(5):1111-9. doi:

   Insert

   sigma 1 (Ap1s1 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHAGE-FKBP-GFP-NAP1

   Plasmid

   #208862

   Purpose

   Stably express GFP-tagged NAP1 for CID induced mitophagy

   Depositor

   Sascha Martens

   Article

   Adriaenssens et al bioRxiv September 25, 202

   Insert

   NAP1 (AZI2 Human)
   

   Use

   Tags

   FKBP-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330-PITCh-MYO1C

    Plasmid

    #227305

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of MYO1C for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting N-terminus of MYO1C (MYO1C Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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