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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 8,237 results for: FIE

Showing: 5441 - 5480 of 8237 results

  1. pT26CfpSox10G4FER3(#1048)

    Plasmid
    #184073
    Purpose
    cyclofen-inducible GAL4 activation in zebrafish permanent transgenic
    Depositor
    Sophie Vriz
    Insert
    Gal4bdFF-ERT2
    Use
    Zebrafish transgenesis (blue heart)
    Tags
    Expression
    Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
    Promoter
    Zf-Sox10
    Available since
    July 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    184073-plasmid-map-sequence-id-365954

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  4. pET_RTX-6xHis

    Plasmid
    #145029
    Purpose
    pET vector for IPTG-inducible, T7-based expression of RTX bearing a C-terminal 6xHis tag
    Depositor
    Andrew Ellington
    Article
    Bhadra et al BioProtocol 2021
    Insert
    RTX
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    145029-plasmid-map-sequence-id-292639

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  6. pDD286 (TagRFP^SEC^3xMyc)

    Plasmid
    #70684
    Purpose
    TagRFP-T^SEC^3xMyc vector with ccdB sites for cloning homology arms.
    Depositor
    Bob Goldstein
    Insert
    TagRFP-T-C1^SEC^3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xMyc and C. elegans codon-optimized TagRFP-T
    Expression
    Worm
    Mutation
    Promoter
    Available since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    70684-plasmid-map-sequence-id-306412

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  7. pFRT-TODestFLAGHA_RNAseH1

    Plasmid
    #65782
    Purpose
    expresses FLAGHA-tagged RNAsH1 in mammalian cells
    Depositor
    Thomas Tuschl
    Insert
    RNaseH1 (RNASEH1 Zebrafish, Human)
    Use
    Tags
    FLAG HA and V5
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, 2x Tet
    Available since
    June 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    65782-plasmid-map-sequence-id-345165

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  8. AAV-hSyn1-GCaMP6f-P2A-nls-dTomato

    Plasmid
    #51085
    Purpose
    Neuronal expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophore
    Depositor
    Jonathan Ting
    Has Service
    AAV Retrograde and AAV1
    Insert
    GCaMP6f
    Use
    AAV
    Tags
    P2A-nls-dTomato
    Expression
    Mammalian
    Mutation
    Promoter
    human Synapsin1
    Available since
    Feb. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    51085-plasmid-map-sequence-id-148706

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  9. AAV-hSyn1-GCaMP6s-P2A-nls-dTomato

    Plasmid
    #51084
    Purpose
    Neuronal expression of GCaMP6s Ca sensor and physically separate nuclear localized dTomato fluorophore
    Depositor
    Jonathan Ting
    Has Service
    AAV Retrograde and AAV1
    Insert
    GCaMP6s
    Use
    AAV
    Tags
    P2A-nls-dTomato
    Expression
    Mammalian
    Mutation
    Promoter
    human Synapsin1
    Available since
    Feb. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    51084-plasmid-map-sequence-id-172966

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  16. pPEE8

    Plasmid
    #128358
    Purpose
    Recycable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by direct repeats.
    Depositor
    James Fraser
    Article
    Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324
    Type
    Empty backbone
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    TEF1
    Available since
    Oct. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    128358-plasmid-map-sequence-id-254573

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  21. pPEE7

    Plasmid
    #128357
    Purpose
    Recycable marker for use in Cryptococcus neoformans. Contains the amdS1 counterselectable marker flanked by direct repeats.
    Depositor
    James Fraser
    Article
    Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324
    Type
    Empty backbone
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    ACT1
    Available since
    Oct. 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    128357-plasmid-map-sequence-id-254608

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  24. pIK86

    Plasmid
    #47933
    Purpose
    C. elegans codon-optimized Cas9 with 2xSV40 NLS sequences. It has a T7 promoter for in vitro transcription, germline-compatible 5'UTR, tbb-2 3'UTR and a polyA sequence.
    Depositor
    Iskra Katic
    Article
    Katic et al Genetics. 2013 Aug 26.
    Insert
    Cas9
    Use
    CRISPR and Unspecified
    Tags
    Expression
    Mutation
    Promoter
    T7
    Available since
    Oct. 3, 2013
    Availability
    Academic Institutions and Nonprofits only
    47933-plasmid-map-sequence-id-68745

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  25. pLVX-EGFP-C3-Cep123

    Plasmid
    #73330
    Purpose
    Lentiviral vector encoding Cep123 isoform 1 with an N-terminal EGFP tag
    Depositor
    Manuel Thery
    Insert
    Cep123 (CEP89 Human)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    73330-plasmid-map-sequence-id-139047

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  28. BDH2_FL_BioN

    Plasmid
    #195870
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    BDH2 (BDH2 Human)
    Use
    Pfbd-bira, baculovirus expression
    Tags
    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG
    Expression
    Mutation
    Promoter
    Polyhedrin
    Available since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    195870-plasmid-map-sequence-id-387243

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  35. pGEM-GluCl_cryst

    Plasmid
    #31487
    Depositor
    Eric Gouaux
    Article
    Hibbs et al Nature. 2011 Jun 2. 474(7349):54-60.
    Insert
    C. elegans glutamate-gated chloride channel alpha, crystallized construct
    Use
    For rna synthesis for expression in x. laevis ooc…
    Tags
    8x-His
    Expression
    Mutation
    Promoter
    Available since
    Sept. 29, 2011
    Availability
    Academic Institutions and Nonprofits only
Showing: 5441 - 5480 of 8237 results