We narrowed to 18,345 results for: MUT

1. pAAV-FLEX-SaCas9-U6-sgGabrg2

   Plasmid

   #124857

   Purpose

   Mutagenesis of Gabrg2

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   Gabrg2 (Gabrg2 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Available Since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMax_mPlum_C207G/T208C

   Plasmid

   #177831

   Purpose

   Site-specific mutagenesis of mPlum for the purposes of creating a site-specific O6-MeG lesion for the purposes of measuring MGMT activity via Host Cell Reactivation (HCR).

   Depositor

   Leona Samson, Zachary Nagel

   Article

   Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

   Insert

   mPlum_C207G/T208C
   

   Expression

   Mammalian

   Mutation

   C207G + T208C

   Promoter

   CMV

   Available Since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. EGFR D837A

   Plasmid

   #11014

   Purpose

   Retroviral construct for expressing human EGFR with D837A mutation in human cells

   Depositor

   Matthew Meyerson

   Article

   Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

   Insert

   EGFR D837A (EGFR Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   D837A

   Available Since

   Dec. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

4. pKLV-EF1aGFP2AKAT7(E508Q)-W

   Plasmid

   #159293

   Purpose

   Lentiviral vector expressing GFP and KAT7 (gRNA-resistant, E508Q mutant) driven by human EF1a promoter

   Depositor

   Kosuke Yusa

   Article

   Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

   Insert

   KAT7 E508Q
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   CRISPR sites mutated (gRNA ID. 5 and A10) and E50…

   Promoter

   human EF1a promoter

   Available Since

   Dec. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. MuHKI-pGFPN3

   Plasmid

   #21919

   Depositor

   Hossein Ardehali

   Article

   Sun et al Mol Cell Biol. 2008 Feb . 28(3):1007-17.

   Insert

   Mutant huamn HKI (with a mutation in the catalytic site in the C-terminal half of the enzyme) in pGFP-N3 (HK1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   This is the full length human HKI cDNA sequence w…

   Available Since

   Nov. 2, 2009

   Availability

   Academic Institutions and Nonprofits only

6. p-spTorA-GFP-H6C

   Plasmid

   #168517

   Purpose

   GFP with the signal peptide of TorA (spTorA) in pBAD24. Includes a C-terminal HHHHHHC (6xHisC) extension.

   Depositor

   Siegfried Musser

   Article

   Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

   Insert

   spTorA-GFP-H6C
   

   Tags

   6xHisC tag and spTorA (signal peptide of E. coli …

   Expression

   Bacterial

   Mutation

   The mut3 GFP variant with 5 additional mutations …

   Promoter

   araBAD

   Available Since

   Oct. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCEP4-BG505.SOSIP-TM

   Plasmid

   #111844

   Purpose

   Mammalian expression plasmid for BG505 SOSIP.664 fused to a transmembrane tether

   Depositor

   Erik Procko

   Article

   Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

   Insert

   HIV-1 Env (BG505 SOSIP.664)
   

   Tags

   CD5 leader sequence and TM helix from MHC class I

   Expression

   Mammalian

   Mutation

   Codon-optimized synthetic gene; has SOSIP mutatio…

   Promoter

   CMV

   Available Since

   March 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5 FRT TO Myc ArA K162R

   Plasmid

   #59805

   Purpose

   Allows the integration of Myc ArA K162R in the genome and Tet-inducible expression

   Depositor

   Stephen Taylor

   Article

   Girdler et al J Cell Sci. 2006 Sep 1;119(Pt 17):3664-7

   Insert

   Aurora A (AURKA Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   K162R, kinase mutant

   Promoter

   hybrid human cytomegalovirus (CMV)/TetO2 promoter

   Available Since

   Feb. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGM190

   Plasmid

   #69614

   Purpose

   inducible gene expression in Streptomyces

   Depositor

   Günther Muth

   Article

   Reuther et al Mol Microbiol. 2006 Jul;61(2):436-46. do

   Inserts

   aphII
   tsr
   rep
   sso
   TipA promoter
   dso
   

   Use

   Streptomyces- e. coli shuttle vector

   Expression

   Bacterial

   Available Since

   Jan. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. ATG-1929

    Plasmid

    #108712

    Purpose

    CBR2opt in pF4Ag expression vector with T7 and CMV promoter

    Depositor

    Keith Wood

    Article

    Hall et al Nat Commun. 2018 Jan 9;9(1):132. doi: 10

    Insert

    CBR2opt
    

    Expression

    Bacterial and Mammalian

    Mutation

    Promega used directed evolution to create CBR (Cl…

    Promoter

    T7, CMV

    Available Since

    Oct. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-FLEX-SaCas9-U6-sgGria2

    Plasmid

    #124854

    Purpose

    Mutagenesis of Gria2

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gria2 (Gria2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-FLAG-MTOR-T1977R

    Plasmid

    #69011

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR-T1977R (MTOR Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    change Threonine 1977 to Arginine

    Available Since

    Dec. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3.1(+)- e18 LOF

    Plasmid

    #131729

    Purpose

    Expresses a loss of function mutant of an engineered variant of RAD18 ("e18")

    Depositor

    Alberto Ciccia

    Article

    Nambiar et al Nat Commun. 2019 Jul 30;10(1):3395. doi:

    Insert

    e18/D221A (Loss of function mutant of RAD18 with SAP domain deleted and UBZ point mutation) (RAD18 Human)
    

    Tags

    FLAG and HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAG27

    Plasmid

    #226742

    Purpose

    Expresses a reporter for a mitochondrial matrix protein

    Depositor

    Jonathan Weissman

    Article

    Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

    Insert

    COX4 (COX4I1 Human)
    

    Use

    Lentiviral

    Tags

    GFP1-10

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGV3341

    Plasmid

    #170279

    Purpose

    Expression of mutant HIV-2 RT p66 reverse transcriptase

    Depositor

    Gregory Van Duyne

    Article

    Sherrill-Mix et al Genome Biol. 2021 Jun 3;22(1):169. doi:

    Insert

    HIV-2 RT p66 (mutant)
    

    Expression

    Bacterial

    Promoter

    T7-lac

    Available Since

    June 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-FLEX-SaCas9-U6-sgGrin2a

    Plasmid

    #124850

    Purpose

    Mutagenesis of Grin2a

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Grin2a (Grin2a Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-FLAG-MTOR-F1888L

    Plasmid

    #69010

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR-F1888L (MTOR Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    change Phenylalanine 1888 to Leucine

    Available Since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pET21b-Caspase-3(D3A: D175A D28A D9A)

    Plasmid

    #90090

    Purpose

    Expresses caspase 3 D3A mutant (D175A D28A D9A) in bacterial cells

    Depositor

    Clay Clark

    Article

    Bose et al Biochemistry. 2003 Oct 28;42(42):12298-3

    Insert

    Caspase 3 (CASP3 Human)
    

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    D175A D28A D9A

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGV3340

    Plasmid

    #170278

    Purpose

    Expression of mutant HIV-2 RT p51 reverse transcriptase

    Depositor

    Gregory Van Duyne

    Article

    Sherrill-Mix et al Genome Biol. 2021 Jun 3;22(1):169. doi:

    Insert

    HIV-2 RT p51 (mutant)
    

    Tags

    Hexahistidine

    Expression

    Bacterial

    Promoter

    T7-lac

    Available Since

    June 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-FLEXfrt-SaCas9-U6-sgSlc17a6

    Plasmid

    #124860

    Purpose

    Mutagenesis of Slc17a6

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc17a6 (Slc17a6 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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