We narrowed to 9,921 results for: pET

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pET29b-ClbPpep-CHis

Plasmid

#48243

Purpose

First 375 amino acids of the peptidase, which contains the soluble periplasmic peptidase domain

Depositor

Emily Balskus

Publication

Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

Insert

ClbP-pep

Tags

6-His

Expression

Bacterial

Mutation

First 375 amino acids of the peptidase, which con…

Promoter

T7

Available since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pET21a-mAIM2-GST

Plasmid

#51533

Purpose

Express GST-tagged mAIM2 in bacteria

Depositor

Emad Alnemri

Publication

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

Aim2 (Aim2 Mouse)

Tags

GST

Expression

Bacterial

Promoter

lac operator

Available since

March 11, 2014

Availability

Academic Institutions and Nonprofits only

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pET21a-hAIM2-GST

Plasmid

#51528

Purpose

Express GST-tagged hAIM2 in bacteria

Depositor

Emad Alnemri

Publication

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

AIM2 (AIM2 Human)

Tags

GST

Expression

Bacterial

Promoter

lac operator

Available since

March 11, 2014

Availability

Academic Institutions and Nonprofits only

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pET28a-Ec.coaBC (pCLK1210)

Plasmid

#50448

Purpose

Heterologous expression of E. coli CoA biosynthetic enzyme, PPCS_PPCDC - phosphopantothenoylcysteine synthetase_phosphopantothenoylcysteine decarboxylase

Depositor

Tadhg Begley, Erick Strauss

Publication

Strauss et al J Biol Chem. 2001 Apr 27;276(17):13513-6

Insert

PPCS PPCDC

Tags

6XHIS tag

Expression

Bacterial

Promoter

T7

Available since

March 6, 2014

Availability

Academic Institutions and Nonprofits only

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pT7CFE1-TelR15-YPet

Plasmid

#49646

Purpose

TALEYellow-Telomere for In Vitro Translation

Depositor

Thoru Pederson

Publication

Ma et al Proc Natl Acad Sci U S A. 2013 Dec 9.

Insert

TelR15

Use

In vitro translation

Tags

YPet

Available since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET11a-p52(1-398)

Plasmid

#44733

Depositor

Gourisankar Ghosh

Insert

p52 (NFKB2 Human)

Expression

Bacterial

Mutation

AA 1-398

Promoter

T7

Available since

July 8, 2013

Availability

Academic Institutions and Nonprofits only
pETCON-IgG1/design_50

Plasmid

#30301

Depositor

David Baker

Publication

Fleishman et al J Mol Biol. 2011 Sep 29.

Insert

IgG Fc Designed Binder IgG1

Expression

Yeast

Available since

June 8, 2011

Availability

Academic Institutions and Nonprofits only
pET20b-delta133HtrA2 S142A

Plasmid

#16153

Depositor

L. Miguel Martins

Publication

Plun-Favreau et al Nat Cell Biol. 2007 Nov;9(11):1243-52. d

Insert

HtrA2 (HTRA2 Human)

Tags

His

Expression

Bacterial

Mutation

S142A

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
pET20b-delta133HtrA2 S400D

Plasmid

#16156

Depositor

L. Miguel Martins

Publication

Plun-Favreau et al Nat Cell Biol. 2007 Nov;9(11):1243-52. d

Insert

HtrA2 (HTRA2 Human)

Tags

His

Expression

Bacterial

Mutation

S400D

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
His6-Twin-Strep-SUMO-pET28a-EfaCas2

Plasmid

#112794

Purpose

To express enterococcus faecalis Cas2 protein in E. coli

Depositor

Ailong Ke

Publication

Xiao et al Nature. 2017 Oct 5;550(7674):137-141. do

Insert

EfaCas2

Tags

His6-Twin-Strep-SUMO

Expression

Bacterial

Available since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pOlyA(WT)

Plasmid

#132391

Purpose

Ostreolysin A for sensing sphingomyelin/cholesterol complexes

Depositor

Arun Radhakrishnan

Publication

Endapally et al Cell. 2019 Feb 21;176(5):1040-1053.e17.

Insert

Ostreolysin A wild-type

Tags

His6 tag

Expression

Bacterial

Mutation

Mutations: C62S, C94S, S151C

Available since

Sept. 16, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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FAT FAK biosensor

Plasmid

#78303

Purpose

FRET biosensor. Visualization of FAK activity at focal adhesions in live cells

Depositor

Yingxiao Wang

Publication

Seong et al Proc Natl Acad Sci U S A. 2013 Nov 26;11

Insert

FAT FAK biosensor

Expression

Mammalian

Promoter

CMV

Available since

June 13, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
V48 ELP

Plasmid

#68395

Purpose

Elastin-like polypeptide (VPGxG) with 48 repeats of valine guest residue

Depositor

Andrew MacKay

Publication

Shah et al Protein Sci. 2012 Jun;21(6):743-50. doi:

Insert

V48 ELP

Expression

Bacterial

Promoter

T7

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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bZiPro

Plasmid

#86846

Purpose

express NS2B-NS3 protease in E.coli

Depositor

Dahai Luo

Publication

Zhang et al Science. 2016 Dec 8. pii: aai9309.

Inserts

NS3 1-177
NS2B45-96

Tags

a hexahistidine tag and a thrombin cleavage site

Expression

Bacterial

Mutation

NS2B cofactor region (residues 45–96) and NS3 pro…

Promoter

T7

Available since

March 2, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pSpCas9_T2A_iRFP670 sgBFP

Plasmid

#192478

Purpose

All-in-one plasmid for cutting BFP region in DSB Spectrum V2 or V3

Depositor

Michael Yaffe

Publication

van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

Insert

SpCas9

Tags

T2A iRFP670

Expression

Mammalian

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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HK2

Plasmid

#25529

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

HK2 (HK2 Human)

Tags

His

Expression

Bacterial

Available since

Sept. 23, 2010

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles
V96 ELP

Plasmid

#68392

Purpose

Elastin-like polypeptide (VPGxG) with 96 repeats of Valine guest residue

Depositor

Andrew MacKay

Publication

Shah et al Protein Sci. 2012 Jun;21(6):743-50. doi:

Insert

V96 ELP

Expression

Bacterial

Promoter

T7

Available since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pET51b-His-TEV-CLIP-tag_fast

Plasmid

#167275

Purpose

Overexpression of the fast variant (E30R) of CLIP-tag in E. coli

Depositor

Kai Johnsson

Publication

Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

Insert

CLIPf-tag

Tags

His x10

Expression

Bacterial

Mutation

E30R

Promoter

T7

Available since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-Fgß-ddFLN4-HIS-ybbr

Plasmid

#101239

Purpose

wildtype Fgß peptide with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forces

Depositor

Hermann Gaub

Publication

Milles et al Science. 2018 Mar 30;359(6383):1527-1533

Insert

Fgß peptide, ddFLN4 fingerprint (FGB Human, D. discoideum)

Tags

HIS and ybbr

Expression

Bacterial

Mutation

C18S in ddFLN4

Promoter

T7

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-GGG-ELP(120nm)-Cys

Plasmid

#91572

Purpose

Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine

Depositor

Hermann Gaub

Publication

Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

Insert

GGG-ELP(120nm)-Cys

Tags

Cysteine and Sortase-Tag

Expression

Bacterial

Promoter

T7

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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