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pDK616

Plasmid

#134202

Purpose

Bacterial expression of human Ska1 microtubule binding domain (MTBD) mutant

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

His-Precission-human Ska1 MTBD (SKA1 Human)

Use

Tags

Expression

Bacterial

Mutation

K223A K226A

Promoter

Available since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pIW040

Plasmid

#134127

Purpose

Bacterial expression of human Ska1 microtubule binding domain (MTBD)

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

His-Precission-human Ska1 MTBD (SKA1 Human)

Use

Tags

Expression

Bacterial

Mutation

aa 132-255 R236A

Promoter

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pIW039

Plasmid

#134126

Purpose

Bacterial expression of human Ska1 microtubule binding domain (MTBD)

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

His-Precission-human Ska1 MTBD (SKA1 Human)

Use

Tags

Expression

Bacterial

Mutation

aa 132-255 and K203A K206A

Promoter

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pIW028

Plasmid

#134125

Purpose

Bacterial expression of human Ska1 microtubule binding domain (MTBD)

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

His-Precission-human Ska1 MTBD (SKA1 Human)

Use

Tags

Expression

Bacterial

Mutation

aa 132-255 and K183A K184A

Promoter

Available since

Feb. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pIW070

Plasmid

#134138

Purpose

Retroviral expression of GFP-Ska1

Depositor

Iain Cheeseman

Article

Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

Insert

GFP-human Ska1 delta MTBD CRISPR/Cas9 hardened (SKA1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

residues 1-131, CRISPR resistant

Promoter

Available since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

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DsRed2-M11-92

Plasmid

#134467

Purpose

M11-92 expression

Depositor

Jennifer Lippincott-Schwartz

Article

Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

Insert

M1 Spastin Amino Acids 1-92 (SPAST Human)

Use

Tags

Expression

Mammalian

Mutation

M1 Spastin Amino Acids 1-92

Promoter

CMV

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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mEmerald-M11-92

Plasmid

#134465

Purpose

M11-92 expression

Depositor

Jennifer Lippincott-Schwartz

Article

Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

Insert

M1 Spastin Amino Acids 1-92 (SPAST Human)

Use

Tags

Expression

Mammalian

Mutation

M1 Spastin Amino Acids 1-92

Promoter

CMV

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-HA-Flag-natT1R2 d835

Plasmid

#113959

Purpose

mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant

Depositor

Erik Procko

Article

Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

Insert

T1R2 (TAS1R2 Human)

Use

Tags

FLAG and Signal/leader sequence from influenza he…

Expression

Mammalian

Mutation

residues 22-834

Promoter

cmv

Available since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pYES260-sc.eEF2 K509A

Plasmid

#85120

Purpose

yeast expression of mutant EEF2

Depositor

Pal Falnes

Article

Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

Insert

EFT1/EFT2 (EFT2, EFT1 Budding Yeast)

Use

Tags

6xHis

Expression

Yeast

Mutation

K509A

Promoter

GAL1

Available since

Nov. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 Dynamin1 P294A

Plasmid

#112102

Purpose

Mammalian expression of GFP-tagged Dynamin protein.

Depositor

Justin Taraska

Article

Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

Insert

Dynamin1 (DNM1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

P294A

Promoter

CMV

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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AY23_pgRNA.R5.2

Plasmid

#100295

Purpose

CCR5-targeting gRNA expression plasmid

Depositor

Manuel Goncalves

Article

Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

Insert

gRNA spacer

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

human U6 Polymerase III snRNA

Available since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_MIER3_2

Plasmid

#86324

Purpose

Encodes gRNA for 3' target of human MIER3

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against MIER3 (MIER3 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_KDM3A_1

Plasmid

#86321

Purpose

Encodes gRNA for 3' target of human KDM3A

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against KDM3A (KDM3A Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only
PX458_HMG20A_2

Plasmid

#86319

Purpose

Encodes gRNA for 3' target of human HMG20A

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against HMG20A (HMG20A Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PX458_KLF11_1

Plasmid

#86314

Purpose

Encodes gRNA for 3' target of human KLF11

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against KLF11 (KLF11 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PX458_MIER3_1

Plasmid

#86323

Purpose

Encodes gRNA for 3' target of human MIER3

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against MIER3 (MIER3 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PX458_KDM3A_2

Plasmid

#86322

Purpose

Encodes gRNA for 3' target of human KDM3A

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against KDM3A (KDM3A Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PX458_HMG20A_1

Plasmid

#86318

Purpose

Encodes gRNA for 3' target of human HMG20A

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against HMG20A (HMG20A Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PX458_KLF11_2

Plasmid

#86315

Purpose

Encodes gRNA for 3' target of human KLF11

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against KLF11 (KLF11 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PX458_KAT7_1

Plasmid

#86309

Purpose

Encodes gRNA for 3' target of human KAT7

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against KAT7 (KAT7 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge