We narrowed to 17,686 results for: SHE

1. p3E_he1a:mCherry

   Plasmid

   #113881

   Purpose

   p3E entry vector for Three Multisite Gateway

   Depositor

   Marnie Halpern

   Insert

   he1a (he1.1 Zebrafish)
   

   Use

   Zebrafish danio expression

   Tags

   mCherry

   Available Since

   Sept. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pChillyBoys_gen1

   Plasmid

   #228495

   Purpose

   pACYCDuet-1 encoding Oleispira antarctica GroEL and GorES low temp chaperonins for enhanced low-temperature induction (E. coli based protein expression)

   Depositor

   Richard Baker

   Inserts

   groEL
   groES
   

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   Jan. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLIX403-ccdB-Blast

   Plasmid

   #158560

   Purpose

   Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

   Depositor

   Alejandro Chavez

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. XLone-PE2

   Plasmid

   #136463

   Purpose

   Expresses prime editing 2 (PE2) enzyme upon doxycycline treatment

   Depositor

   Xiaojun Lian

   Insert

   PE2
   

   Expression

   Mammalian

   Promoter

   TRE3GS

   Available Since

   May 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. CROP-seq-opti-dsRed

   Plasmid

   #201999

   Purpose

   Based on CROP-seq-opti (addgene #106280), we replaced the PURO resistance gene with a dsRed.

   Depositor

   Daniel Geschwind

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   dsRed

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGMC00010 (aka pRC0495)

   Plasmid

   #195295

   Purpose

   Lenti-CRISPR-V2-RFP-2A-Puro - vector with Cas9/sgRNA backbone/Puro/RFP

   Depositor

   Raj Chari

   Type

   Empty backbone

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Expression

   Mammalian

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDual_dsCas9_Blast

   Plasmid

   #214681

   Purpose

   Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two empty sgRNA cloning sites

   Depositor

   Kevin Janes

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Available Since

   Dec. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEM4Z-OMA1

   Plasmid

   #61718

   Purpose

   human Oma1 protease in the E.coli/in vitro translation vector

   Depositor

   Oleh Khalimonchuk

   Insert

   OMA1 (OMA1 Human)
   

   Expression

   Bacterial

   Available Since

   Dec. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pLenti-hSyn1-mRuby2-T2A-GCaMP6f

   Plasmid

   #197595

   Purpose

   Lentiviral transfer vector for the neuronal expression of mRuby2-T2A-GCaMP6f (fluorescent reporter for Calcium imaging)

   Depositor

   Patrik Verstreken

   Insert

   mRuby2-T2A-GCaMP6f
   

   Use

   Lentiviral

   Promoter

   hSynI

   Available Since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBlu-CGT-1

    Plasmid

    #170158

    Purpose

    Expresses bacterial Cyclomaltodextrin Glucanotransferase from Paenibacillus sp. c36 in E.coli

    Depositor

    Sarah MacDonald

    Insert

    Cyclomaltodextrin Glucanotransferase
    

    Expression

    Bacterial

    Promoter

    Tac

    Available Since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBHt2G-RFP

    Plasmid

    #107162

    Purpose

    Golden-Gate compatible fungal transformation vector carrying Hygromycin Resistance w RFP screening cassette (RFP knocked out by GG reaction, allowing for red-white screening)

    Depositor

    Sophien Kamoun

    Type

    Empty backbone

    Use

    Fungal grobacterium transformation plasmid, teste…

    Available Since

    March 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBIR-GFP

    Plasmid

    #49807

    Purpose

    In vivo GFP based system to measure break induced replication (BIR) repair efficiency. expression of I-SceI generates a double strand break that when repaired by BIR mechanism will restore GFP.

    Depositor

    Thanos Halazonetis

    Insert

    GFP
    

    Expression

    Mammalian

    Promoter

    beta-actin

    Available Since

    Jan. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGR481

    Plasmid

    #213783

    Purpose

    Integrates onto p1 a catalytically dead TRP5 and URA3 that can be used for selection of transversion mutations

    Depositor

    Chang Liu

    Insert

    ura3*
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Available Since

    March 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRSET.HaloTag.v7.AviTag

    Plasmid

    #195486

    Purpose

    Bacterial expression plasmid with Halotag v7 insert plus a C-terminus appended AviTag for enzymatic biotinylation.

    Depositor

    HHMI-JRC Tool Translation Team, Tim Brown

    Insert

    HaloTag.v7.AviTag
    

    Tags

    6xHIS and AviTag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGR475

    Plasmid

    #213784

    Purpose

    Integrates at the CAN1 locus the WT TP-DNAP1 and the Hygromycin resistance marker

    Depositor

    Chang Liu

    Insert

    WT TP-DNAP1 (recoded)
    

    Use

    Synthetic Biology

    Expression

    Yeast

    Available Since

    March 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRRlsinPGK_CREGFP_WPRE

    Plasmid

    #91789

    Purpose

    Constitutive expression of creGFP fusion using PGK promoter in a lentiviral backbone.

    Depositor

    Beverly Torok-Storb

    Insert

    Cre-GFP fusion protein
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. LentiCRISPRv2 RFP670

    Plasmid

    #187646

    Purpose

    lentiviral vector expressing RFP670 alongside Cas9 and an sgRNA cloning site

    Depositor

    Martin Carroll

    Insert

    RFP 670
    

    Use

    Lentiviral

    Tags

    RFP670 and sgRNA cloning site

    Promoter

    EFS (P2A)

    Available Since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. PB CAG-iCre-ERT2

    Plasmid

    #137858

    Purpose

    CAG-iCre-ERT2 piggyBac transposon

    Depositor

    Mario Capecchi

    Insert

    iCre-ERT2
    

    Use

    Cre/Lox; Piggybac

    Expression

    Mammalian

    Available Since

    Nov. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. CMV-mt4xi-GCaMP6f

    Plasmid

    #192075

    Purpose

    Fluorescent reporter for mitochondrial calcium

    Depositor

    Timothy Ryan

    Insert

    4 repeats of improved COX8 signal peptide plus GCaMP6f
    

    Expression

    Mammalian

    Available Since

    Dec. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSpot-Tag-GFP-GPI

    Plasmid

    #117061

    Purpose

    For mammalian expression of GPI-anchored GFP with N-terminal Spot-Tag

    Depositor

    Felix Hartlepp

    Insert

    GPI-anchored GFP
    

    Tags

    GPI-anchor and Spot-Tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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21. pBabe-EGFP-Hu_Plk1(WT)

    Plasmid

    #136323

    Purpose

    Retroviral expression of human Plk1-WT with N-terminal EGFP

    Depositor

    Guillermo de Cárcer, Marcos Malumbres

    Insert

    PLK1 (PLK1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. pTYB1-T4uvsX

    Plasmid

    #228213

    Purpose

    Expression in BL21(DE3) of T4 recombinase

    Depositor

    Stephen Benkovic

    Insert

    uvsX (uvsX Escherichia phage T4)
    

    Tags

    Sce VMA intein-chitin binding domain

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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23. SPLICSFAST-ER-PM-P2A

    Plasmid

    #214893

    Purpose

    Expresses equimolar Plasma Membrane- and Endoplasmic Reticulum- targeted split-FAST fragments for Short-range ER-PM Contact sites

    Depositor

    Tito Calì

    Insert

    SPLICSFAST-ER-PM-P2A
    

    Tags

    none

    Expression

    Bacterial and Mammalian

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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24. CYP2C19

    Plasmid

    #69606

    Purpose

    For protein expression of CYP2C19

    Depositor

    Joyce Goldstein

    Insert

    CYP2C19 (CYP2C19 Human)
    

    Expression

    Bacterial

    Mutation

    deletion of AA1-8 and mutation I331V

    Available Since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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25. pJK645

    Plasmid

    #71700

    Purpose

    Produces E. coli thioredoxin (EcTrxA)

    Depositor

    T. J. Kappock

    Insert

    thioredoxin
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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26. pMCMV3

    Plasmid

    #85711

    Purpose

    Expression vector employing the mouse CMV major immediate-early promoter enhancer (MIEP); there is a small polylinker (PstI, SmaI, XbaI, SalI ,HpaI) followed by the SV40 polyA signal sequences

    Depositor

    Ulrich Koszinowski, Martin Messerle

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    MIEPmouse CMV MIEP

    Available Since

    April 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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27. pAAV-syn-DIO-axon-jGCaMP8f

    Plasmid

    #220531

    Purpose

    For Cre-dependent enriched expression of GCaMP8f in axons

    Depositor

    Lin Tian

    Insert

    Axon-DIO-GCaMP8f
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSynapsin

    Available Since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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28. pCambia-dLB-kanR-GFP

    Plasmid

    #223177

    Purpose

    Plant Transformation Vector that allows marker removal through Mendelian inheritance. Start of DNA transfer at RB, terminates at left border but in ~1/3 of cases goes ahead until second left border

    Depositor

    Andreas Stuermer

    Type

    Empty backbone

    Use

    Ti plasmid for agrobacterium

    Expression

    Plant

    Available Since

    Aug. 26, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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29. pAG GST EGFP

    Plasmid

    #236736

    Purpose

    Bacterial expression plasmid containing GST-tagged EGFP

    Depositor

    Addgene Research Program, Melina Fan

    Insert

    EGFP
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    Tac

    Available Since

    April 14, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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30. p3E_he1a:eYFP

    Plasmid

    #113879

    Purpose

    p3E entry vector for Three Multisite Gateway

    Depositor

    Marnie Halpern

    Insert

    he1a (he1.1 Zebrafish)
    

    Use

    Zebrafish danio expression

    Tags

    eYFP

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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