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Plasmid#128202PurposecrRNA backbone for LbCas12a, combine with TaU3 promoter module (pFH33)DepositorInsertcrRNA backbone for LbCas12a, combine with TaU3 promoter module (pFH33)
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFH70
Plasmid#128190PurposeLevel1 ZmUBIp::nSpCas9-PmCDA (Arabidopsis codon optimized):NOStDepositorInsertZmUBIp::nSpCas9-PmCDA (Arabidopsis codon optimized):NOSt
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCF704
Plasmid#121658PurposeU6-sgRNA EFS-ProCas9-Flavi-P2A-PuroDepositorInsertCas9(C)
UseLentiviralTagsExpressionMutationPromoterU6Available sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCF712
Plasmid#121663PurposeU6-sgRNA EF1a-ProCas9-Flavi-P2A-PuroDepositorInsertCas9(C)
UseLentiviralTagsExpressionMutationPromoterU6Available sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0114
Plasmid#117553PurposeLevel1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting NbPDS gene in N. benthamianaDepositorInsertPromoter_AtU6-26 + sgRNA_NbPDS1 N. benthamiana
UseTagsExpressionPlantMutationPromoterAt U6-26 (pICSL90002)Available sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0029
Plasmid#117541PurposeLevel1 Golden Gate Cassette: sgRNA cassette for LbCas12a, targeting NbPDS gene in N. benthamianaDepositorInsertcrRNA (LbCas12a)
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0028
Plasmid#117540PurposeLevel1 Golden Gate Cassette: sgRNA cassette for AsCas12a, targeting NbPDS gene in N. benthamianaDepositorInsertcrRNA (AsCas12a)
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0014
Plasmid#117524PurposeLevel0 Golden Gate part: CDS, SpCas9-KADepositorInsertSpCas9-KA (with stop codon)
UseCRISPRTagsExpressionMutationK855APromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0009
Plasmid#117522PurposeLevel0 Golden Gate part: CDS, SpCas9-p (no stop codon)DepositorInsertSpCas9-p (no stop codon)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pICSL90005
Plasmid#117520PurposeLevel0 Golden Gate part: CDS, SpCas9-h (no stop codon)DepositorInsertSpCas9-h (no stop codon)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0008
Plasmid#117535PurposeLevel0 Golden Gate part: CDS, AsCas12aDepositorInsertAsCas12a (with stop codon)
UseCRISPRTags3xHA and NLSExpressionMutationPromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNB2629
Plasmid#113394PurposeMET25p-MS2-RFP-CYC1 TTDepositorInsertMS2 bacteriophage coat protein
UseTagsred fluorescent protein (RFP), codon-optimized fo…ExpressionYeastMutationPromoterAvailable sinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
KG#244
Plasmid#110927PurposeVector for expressing proteins in a subset of 9 DA/ DB cholingergic motor neurons in the C. elegans ventral nerve cordDepositorInsertsunc-129 promoter
unc-54 3' control region
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
FGFR2 K659M 3xFlag
Plasmid#110112Purposeexpresses FGFR2 with a single amino acid change in mammalian cellsDepositorInsertfibroblast growth factor receptor 2 (FGFR2 Human)
UseTagsFlagExpressionMammalianMutationmutated Lysine 659 to Methiionine (K659M)PromoterCMVAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
FGFR2 E565A 3xFlag
Plasmid#110109Purposeexpresses FGFR2 with a single amino acid change in mammalian cellsDepositorInsertfibroblast growth factor receptor 2 (FGFR2 Human)
UseTagsFlagExpressionMammalianMutationmutated Glutamic acid 565 to Alanine (E565A)PromoterCMVAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
FGFR2 K641R 3xFlag
Plasmid#110111Purposeexpresses FGFR2 with a single amino acid change in mammalian cellsDepositorInsertfibroblast growth factor receptor 2 (FGFR2 Human)
UseTagsFlagExpressionMammalianMutationmutated Lysine 641 to Arginine (K641R)PromoterCMVAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
FGFR2 N549H 3xFlag
Plasmid#110106Purposeexpresses FGFR2 with a single amino acid change in mammalian cellsDepositorInsertfibroblast growth factor receptor 2 (FGFR2 Human)
UseTagsFlagExpressionMammalianMutationmutated Asparagine 549 to Histidine (N549H)PromoterCMVAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
FGFR2 N549K 3xFlag
Plasmid#110107Purposeexpresses FGFR2 with a single amino acid change in mammalian cellsDepositorInsertfibroblast growth factor receptor 2 (FGFR2 Human)
UseTagsFlagExpressionMammalianMutationmutated Asparagine 549 to Lysine (N549K)PromoterCMVAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
FGFR2 L617V 3xFlag
Plasmid#110110Purposeexpresses FGFR2 with a single amino acid change in mammalian cellsDepositorInsertfibroblast growth factor receptor 2 (FGFR2 Human)
UseTagsFlagExpressionMammalianMutationmutated Leucine 617 to Valine (L617V)PromoterCMVAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXT
Plasmid#107574PurposeContains the VGF promoter (about 700 bp) plus 800 bp of 5' untranslated sequence. The HindIII (5')-HincII(3') fragment acts as a NGF-inducible promoterDepositorInsertVGF promoter (Vgf Rat)
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9 (BB)-2A-GFP gamma-tubulin sg
Plasmid#104437PurposeKnockdown the expression of gamma-tubulin in human cellsDepositorInsertgamma-tubulin sgRNA
UseTagsThe gamma-tubulin sgRNA is coexpressed with 3xFLA…ExpressionMammalianMutationPromoterAvailable sinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTC102
Plasmid#91176Purposea derivative of pDIRECT_37J with translation initiation sequence optimized for dicotsDepositorInsertEngineering Reagent: 35S:TALEN
UseTALENTagsExpressionMutationPromoterAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSC1
Plasmid#91179PurposeT-DNA vector expressing TALENs targeting Medtr4g020620 and Medtr2g013650ADepositorInsertTALENs targeting Medtr4g020620 and Medtr2g013650A
UseTALENTagsExpressionPlantMutationPromoterAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A3013
Plasmid#91046PurposeModule A, Promoter: PvUbi2, Gene: GFP, Terminator: HSPDepositorInsertGFP
UseUnspecifiedTagsExpressionMutationPromoterPvUbi2Available sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMOD_A3014
Plasmid#91047PurposeModule A, Promoter: Nos, Gene: GFP, Terminator: HSPDepositorInsertGFP
UseUnspecifiedTagsExpressionMutationPromoterNosAvailable sinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
ReNL-Actin_pcDNA3
Plasmid#89531PurposeRed color variant of bright luminescent protein, fused to actinDepositorInsertReNL
UseTagsActinExpressionMammalianMutationPromoterCMVAvailable sinceMay 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
tubulin-OeNL_pcDNA3
Plasmid#89523PurposeOrange color variant of bright luminescent protein, fused to b-tubulinDepositorInsertOeNL
UseTagsb-tubulinExpressionMammalianMutationPromoterCMVAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVHed07
Plasmid#82350PurposeBxb1/TP901 temporal logic gate with mKate2 and sfGFP in Phi80 chromosomal integration plasmidDepositorInsertsmKate2 RFP
Superfolder GFP
Temporal logic gate with TP901 and Bxb1 attachment sites
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJan. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDGE63
Plasmid#79445Purposerecipient plasmid [single nuclease]; p35S:Cas9, BASTADepositorInsertccdB (ccdB(letD) )
UseCRISPRTagsExpressionPlantMutationBsaI site eliminatedPromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGE144
Plasmid#79448Purposerecipient plasmid [multiplexing]; p35S:Cas9, FAST, BASTADepositorInsertccdB (ccdB(letD) )
UseCRISPRTagsExpressionPlantMutationBsaI site eliminatedPromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only