We narrowed to 9,154 results for: pho;

1. CPH3567 (pET151_Spt6 1247-1451 R1282H)

   Plasmid

   #91816

   Purpose

   Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with an R1282H mutation

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   SPT6 (SPT6 Budding Yeast)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   changed arginine 1282 to histidine; deleted resid…

   Promoter

   T7

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. CPH3495 (pET151_Spt6 1247-1451 K1435A)

   Plasmid

   #91817

   Purpose

   Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with a K1435A mutation

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   SPT6 (SPT6 Budding Yeast)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   changed lysine 1435 to alanine; deleted residues …

   Promoter

   T7

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. KIF13B

   Plasmid

   #163907

   Purpose

   Full length mouse KIF13B for purification from insect cells

   Depositor

   Martin Loose

   Article

   Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

   Insert

   kif13b (Kif13b Mouse)
   

   Use

   Tags

   Cleavable 6 x HIS tag

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. KIF13B 1-584

   Plasmid

   #163908

   Purpose

   Motile fragment of mouse KIF13B (aa 1-584) for purification from insect cells.

   Depositor

   Martin Loose

   Article

   Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

   Insert

   kif13b (Kif13b Mouse)
   

   Use

   Tags

   Cleavable 6 x HIS tag

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHA853 - mec-4p::Fynempty

   Plasmid

   #139210

   Purpose

   Control vector for Fyn kinase expression in mec-4 neurons

   Depositor

   Anne Hart

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   Fyn (FYN Human)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   deletion and frame shift to prevent Fyn expression

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHA850 - osm-10p::FynY531F

   Plasmid

   #139207

   Purpose

   Express activated Fyn kinase in osm-10 neurons

   Depositor

   Anne Hart

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   Fyn (FYN Human)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Y531F

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHA851 - osm-10p::Fynempty

   Plasmid

   #139208

   Purpose

   Control vector for Fyn kinase expression in osm-10 neurons

   Depositor

   Anne Hart

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   Fyn (FYN Human)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   deletion and frame shift to prevent Fyn expression

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. FUS_LC_withR

   Plasmid

   #139128

   Purpose

   express FUS LC with the same number and spacing of arginines as hnRNPA2 LC

   Depositor

   Nicolas Fawzi

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   FUS (FUS Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   add arginines

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. hnRNPA2_LC_nocharge_wR

   Plasmid

   #139119

   Purpose

   express hnRNPA2 LC with no charged residues with R added back

   Depositor

   Nicolas Fawzi

   Article

   Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

   Insert

   hnRNPA2 (HNRNPA2B1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   remove all K, most D

   Promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. FUS_LC_charge

    Plasmid

    #139126

    Purpose

    express FUS LC with an hnRNPA2 LC-like charge patterning

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Add R, K, D to make FUS LC have similar charge pa…

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLenti-CMV-TIRAP-cGASΔN-HA

    Plasmid

    #130921

    Purpose

    Expresses human cGASΔN (aa160-522)-HA with an N terminal fusion of the TIRAP N terminus (aa1-85); Puromycin selection marker

    Depositor

    Jonathan Kagan

    Article

    Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

    Insert

    TIRAP-ΔNcGAS
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    cGAS N terminus (aa1-159) replaced with the N ter…

    Promoter

    CMV

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET151-GST-PP-RPN13 (19-132) R104A

    Plasmid

    #125826

    Purpose

    Expresses GST-RPN13 (19-132) R104A in E.coli

    Depositor

    Chris Hill

    Article

    Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

    Insert

    RPN13 (ADRM1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    changed arginine 104 to alanine

    Promoter

    T7

    Available since

    May 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET151-GST-PP-RPN13 (19-132) R104D

    Plasmid

    #125827

    Purpose

    Expresses GST-RPN13 (19-132) R104D in E.coli

    Depositor

    Chris Hill

    Article

    Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

    Insert

    RPN13 (ADRM1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    changed arginine 104 to aspartate

    Promoter

    T7

    Available since

    May 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET151-GST-PP-RPN13 (19-132) R104E

    Plasmid

    #125828

    Purpose

    Expresses GST-RPN13 (19-132) R104E in E.coli

    Depositor

    Chris Hill

    Article

    Hemmis et al J Biol Chem. 2019 May 7. pii: AC119.0088

    Insert

    RPN13 (ADRM1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    changed arginine 104 to glutamate

    Promoter

    T7

    Available since

    May 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Gal4-HSF4b K294R

    Plasmid

    #118359

    Purpose

    This plasmid expresses amino-acids 200-493 of hHSF4b K294R mutant fused to the GAL-4 DNA binding domain. This construct cannot undergo sumoylation on K294.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

    Insert

    hHSF4b (HSF4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K294R

    Promoter

    Available since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Flag-KSR R615H

    Plasmid

    #118332

    Purpose

    Expression of mouse KSR

    Depositor

    Philip Stork

    Article

    Takahashi et al J Biol Chem. 2017 Jan 27;292(4):1449-146

    Insert

    KSR R615H (Ksr1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    R615H

    Promoter

    Available since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. hHSF1 S307A

    Plasmid

    #118364

    Purpose

    This plasmid expresses human HSF1 S307A mutant.

    Depositor

    Lea Sistonen

    Article

    Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

    Insert

    hHSF1 (HSF1 Human)
    

    Use

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    S307A

    Promoter

    Available since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCDNA3_PKA-Cα(S11D)-mPAGFP

    Plasmid

    #114135

    Purpose

    Mammalian expression of the S11D mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.

    Depositor

    Haining Zhong

    Article

    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

    Insert

    the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP with S11D mutation (Prkaca Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCDNA3_PKA-Cα(S11A)-mPAGFP

    Plasmid

    #114134

    Purpose

    Mammalian expression of the S11A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.

    Depositor

    Haining Zhong

    Article

    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

    Insert

    the mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP with S11A mutation (Prkaca Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDNA3_PKA-CαN-mPAGFP

    Plasmid

    #114114

    Purpose

    Mammalian expression of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP.

    Depositor

    Haining Zhong

    Article

    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

    Insert

    the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric paGFP (Prkaca Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge