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GAK_HUMAN_D0

Plasmid

#79682

Purpose

This plasmid encodes the kinase domain of GAK. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

Depositor

John Chodera, Nicholas Levinson, Markus Seeliger

Article

Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

Insert

GAK (GAK Human)

Tags

His10-TEV

Expression

Bacterial

Promoter

T7

Available Since

Nov. 21, 2016

Availability

Academic Institutions and Nonprofits only
pDONR223_STAG2_p.L997W

Plasmid

#81588

Purpose

Gateway Donor vector containing STAG2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

STAG2 (STAG2 Human)

Use

Gateway Cloning

Mutation

L997W

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_STAG2_p.R252Q

Plasmid

#81589

Purpose

Gateway Donor vector containing STAG2 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

STAG2 (STAG2 Human)

Use

Gateway Cloning

Mutation

R252Q

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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p-HITI-F9-Ckm

Plasmid

#226120

Purpose

HITI insert construct with Ckm gRNA and hF9 transgene

Depositor

Christopher Nelson

Article

Padmaswari et al Mol Ther Nucleic Acids. 2024 Sep 2;35(4)

Inserts

F9 (F9 Human)
Ckm gRNA (Ckm Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 4, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pAcGFP-N1_CBPKTG

Plasmid

#221911

Purpose

Transient expression of GFP-tagged CBPKTG in mammalian cells

Depositor

Daniel Bose

Article

Gelder et al Cell Rep. 2026 Mar 12;45(3):117109. doi:

Insert

CBP (CREBBP Human)

Tags

GFP

Expression

Mammalian

Mutation

mutation of lysine residues to glycine residues (…

Available Since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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pAcGFP-N1_CBPdIDR6KTG

Plasmid

#221913

Purpose

Transient expression of GFP-tagged CBPdeltaIDR6KTG in mammalian cells

Depositor

Daniel Bose

Article

Gelder et al Cell Rep. 2026 Mar 12;45(3):117109. doi:

Insert

CBP (CREBBP Human)

Tags

GFP

Expression

Mammalian

Mutation

deletion of IDR6 (aa 1856 - 2090); mutation of ly…

Available Since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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pAcGFP-N1_CBPdIDR7KTG

Plasmid

#221915

Purpose

Transient expression of GFP-tagged CBPdeltaIDR7KTG in mammalian cells

Depositor

Daniel Bose

Article

Gelder et al Cell Rep. 2026 Mar 12;45(3):117109. doi:

Insert

CBP (CREBBP Human)

Tags

GFP

Expression

Mammalian

Mutation

deletion of IDR7 (aa 2109-2434); mutation of lysi…

Available Since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-ZNF805

Plasmid

#101589

Purpose

Donor Vector containing ZNF805 transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF805 (ZNF805 Human)

Use

Gateway Cloning

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

June 3, 2021

Availability

Academic Institutions and Nonprofits only

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pEf1a-hifiDn29-dCas9-P2A-GFP

Plasmid

#247158

Purpose

Mammalian expression of a human codon optimized engineered hifiDn29 recombinase fused to dCas9 and gRNA expression cassette for targeting attH1 with H1-g3

Depositor

Patrick Hsu

Article

Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

Insert

hifiDn29-dCas9-P2A-GFP

Tags

SV40 NLS

Expression

Mammalian

Mutation

M6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503N/I98V…

Promoter

Ef1a

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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DNA-PKcs T3950D

Plasmid

#83320

Purpose

DNA-PKcs T3950A

Depositor

Katheryn Meek

Article

Douglas et al Mol Cell Biol. 2007 Mar;27(5):1581-91. E

Insert

human DNA-PKcs (PRKDC Human)

Expression

Mammalian

Mutation

Activation loop phosphorylation site 3950 substit…

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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