We narrowed to 7,333 results for: ef1a
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Plasmid#43889PurposeActivation Domain: NF-kB; RVD of C-terminal 0.5 TALE repeat: HD (binds C)DepositorInsertTALE-activator p65 backbone
UseTale transcriptional activator expression vectorPromoterEF1alphaAvailable SinceMarch 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
MLM2581
Plasmid#43888PurposeActivation Domain: NF-kB; RVD of C-terminal 0.5 TALE repeat: NI (binds A)DepositorInsertTALE-activator p65 backbone
UseTale transcriptional activator expression vectorPromoterEF1alphaAvailable SinceMarch 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
MLM3583
Plasmid#43887PurposeActivation Domain: VP64; RVD of C-terminal 0.5 TALE repeat: NG (binds T)DepositorInsertTALE-activator VP64 Backbone
UseTale transcriptional activator expression vectorPromoterEF1alphaAvailable SinceMarch 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
MLM2585
Plasmid#43890PurposeActivation Domain: NF-kB; RVD of C-terminal 0.5 TALE repeat: NN (binds G)DepositorInsertTALE-activator p65 backbone
UseTale transcriptional activator expression vectorPromoterEF1alphaAvailable SinceMarch 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
MLM3587
Plasmid#43886PurposeActivation Domain: VP64; RVD of C-terminal 0.5 TALE repeat: NN (binds G)DepositorInsertTALE-activator VP64 Backbone
UseTale transcriptional activator expression vectorPromoterEF1alphaAvailable SinceMarch 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4xh7SKPylT_EF1_BsmBI_Intein_DD
Plasmid#162810PurposeSTELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient expressionDepositorInsertBsmBI_Intein_DD
UsePiggybacExpressionMammalianPromoterEF1alphaAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-*GFP
Plasmid#162806PurposeSTELLA plasmid for amber suppression of N-terminally labelled GFPDepositorInsertEF1_Ub-(N-TAG)GFP
UsePiggybacExpressionMammalianMutationGFP (Met1,Amber)PromoterEF1alphaAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSL1103
Plasmid#129526PurposepDEF-SpCas9, No Target Control Vector for CRISPRi, PbEF1a promoter, 1st GenerationDepositorInsertdSpCas9
UsePlasmodium, rodent-infectious speciesTagsNLS, 1xHAAvailable SinceSept. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSL1315
Plasmid#129527PurposepDEF-SpCas9, Best (-458) Vector for CRISPRi of PyALBA4, PbEF1a promoter, 1st GenerationDepositorInsertdSpCas9
UsePlasmodium, rodent-infectious speciesTagsNLS, 1xHAAvailable SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTBL3328 PB-sfGFP-WPRE-target
Plasmid#226676PurposePiggyBac cargo vector encoding sfGFP followed by WPRE and a transcribed peCHYRON target site.DepositorInsertsfGFP-WPRE-site3target
ExpressionMammalianPromoterEF1alphaAvailable SinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSL1419
Plasmid#219778PurposepDEF-TurboID-GFPmut2, negative control for cytosolic expressionDepositorInsertTurboID/GFPmut2
UsePlasmodium, rodent malaria parasitesPromoterPbEF1alpha promoterAvailable SinceJune 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
aCE106
Plasmid#204496PurposeSmall molecule helper circuitDepositorInsertsrtTA3
PhIF-ABI
VPR-Pyl1
UseSynthetic BiologyExpressionBacterial and MammalianPromoterhEF1aAvailable SinceDec. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
FB407
Plasmid#203628PurposePromoter of the elongation factor 1-alpha gene from P. digitatum (PDIG_59570).DepositorInsertPef1A
UseSynthetic BiologyAvailable SinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
C413-E19: attB4-mPol2p>-attB1r
Plasmid#162971PurposeGateway attB4/attB1r entry clone for mammalian expression using the human EF1alpha promoterDepositorInsertmurine RNA polymerase 2 promoter
UseSynthetic BiologyAvailable SinceApril 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4xh7SKPylT_EF1_SMIM38*_Intein_DD
Plasmid#162814PurposeSTELLA plasmid for amber suppression of C-terminally labelled SMIM38DepositorInsertSMIM38(C-TAG)-Intein-DD
UsePiggybacExpressionMammalianMutationSMIM38(Ochre52,Amber)PromoterEF1alphaAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub(G75,76A)-BsmBI
Plasmid#162803PurposeControl STELLA destination plasmid for N-terminally labelled proteinsDepositorInsertUb(G75,76A)-BsmBI
UsePiggybacExpressionMammalianMutationUbiquitin (G75,76A)PromoterEF1alphaAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-*SMIM38
Plasmid#162808PurposeSTELLA plasmid for amber suppression of N-terminally labelled SMIM38DepositorInsertUb-(N-TAG)SMIM38
UsePiggybacExpressionMammalianMutationSMIM38 (Met1, Amber)PromoterEF1alphaAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS_4x7SKPylT_EF1_Ub-*PIGBOS
Plasmid#162809PurposeSTELLA plasmid for amber suppression of N-terminally labelled PIGBOSDepositorInsertUb-(N-TAG)PIGBOS
UsePiggybacExpressionMammalianMutationPIGBOS (Met1, Amber)PromoterEF1alphaAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHAGE EF1α dCas9-KRAB
Plasmid#50919PurposeConstitutive dCas9-KRAB 2nd generation lentiviral expression vectorDepositorInsertdCas9
UseCRISPR and LentiviralTags3xHA and KRABExpressionMammalianMutationD10A, H840APromoterEF1alphaAvailable SinceMarch 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
PB-CT3G-CERP2-MG
Plasmid#175503PurposeAll-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionMammalianPromoterTRE3G-mCMVAvailable SinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB303
Plasmid#68631PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneExpressionPlantPromoterMpEF1alpha promoterAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB203
Plasmid#68594PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneExpressionPlantPromoterMpEF1alpha promoterAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
alpha7nACHR_Nacho_mCherry
Plasmid#182939Purposealpha7 nACHR expression in mammalian cell linesDepositorAvailable SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-MCS-3AID-HA-2A-mCherryBSD
Plasmid#225700PurposeEmpty backbone for lentiviral expression of AID degron tagged POI and mCherry fused BlasticidinRDepositorTypeEmpty backboneUseLentiviral and Synthetic BiologyTagsT2A-mCherryExpressionMammalianPromoterpEF1AAvailable SinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB-CT3G-ERP2
Plasmid#175497PurposeAll-in-one piggyBac transposon vector for mCMV+dox-inducible expression of Golden Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionMammalianPromoterTRE3G-mCMVAvailable SinceOct. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE EF1α dCas9-VP64
Plasmid#50918PurposeConstitutive dCas9-VP64 lentiviral expression vectorDepositorInsertdCas9
UseCRISPR and LentiviralTags3xHA and VP64ExpressionMammalianMutationD10A, H840APromoterEF1alphaAvailable SinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pHR-OFF VIPER CAR (anti-CD19)-candidate design(d+i)
Plasmid#192123PurposeLentiviral deliviery of OFF VIPER CAR (anti-CD19)-candidate design(d+i)DepositorInsertpEF1a-anti-CD19scFV_CD28__41BB_peptide_P2A_DAP10_41BB_NS3(s139a)_CD3z-mCherry
UseLentiviralAvailable SinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-3XMyc-mTrim25
Plasmid#51394DepositorAvailable SinceMarch 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pMpGWB103
Plasmid#68557PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneExpressionPlantPromoterMpEF1alpha promoterAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB110
Plasmid#68564PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTags3xFLAGExpressionPlantPromoterMpEF1alpha promoterAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB108
Plasmid#68562PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsCitrineExpressionPlantPromoterMpEF1alpha promoterAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pHR-ON VIPER CAR (anti-CD19)-final design
Plasmid#192117PurposeLentiviral deliviery of ON VIPER CAR (anti-CD19)-final designDepositorInsertpHR-pEF1a-VIPER CAR (anti-CD19scFV_CD28_NS3_41BB_CD3z)
UseLentiviralAvailable SinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
P2A-NLS-tdtomato-EPG backbone
Plasmid#215548PurposeAn empty backbone for cloning of homology arms; ITR-flanked, EF1alpha-driven expression of puromycin-EGFPDepositorTypeEmpty backboneUseCRISPRAvailable SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
P2A-mCherry-EhT backbone
Plasmid#215549PurposeAn empty backbone for cloning of homology arms; ITR-flanked, EF1alpha-driven expression of hygromycin-NLS-tdTomatoDepositorTypeEmpty backboneUseCRISPRAvailable SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
BII-ChBtW-iresFUCCI
Plasmid#133371PurposePiggybac vector for constitutive expressionDepositorInsertClover-Geminin-IRES-mKO2-Cdt1
TagsFlagExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available SinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB408
Plasmid#68673PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsCitrineExpressionPlantPromoterMpEF1alpha promoterAvailable SinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB127
Plasmid#68581PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsTagRFPExpressionPlantPromoterMpEF1alpha promoterAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
PB-CT3G-ERP2-MG
Plasmid#175501PurposeAll-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionMammalianPromoterTRE3G-mCMVAvailable SinceOct. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB236
Plasmid#68627PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsTagRFPExpressionPlantPromoterMpEF1alpha promoterAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB313
Plasmid#68641PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsGlucocorticoid receptor (GR)ExpressionPlantPromoterMpEF1alpha promoterAvailable SinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEF HaloTag-SYB2
Plasmid#197282PurposeEncodes full-length Synaptobrevin 2 (SYB2) with mutations Q76V and F77W to confer resistance to tetanus cleavage.DepositorAvailable SinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
PB-CT3G-CERP2
Plasmid#175499PurposeAll-in-one piggyBac transposon vector for mCMV+dox-inducible expression of Golden Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionMammalianPromoterTRE3G-mCMVAvailable SinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB113
Plasmid#68567PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsGlucocorticoid receptor (GR)ExpressionPlantPromoterMpEF1alpha promoterAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB308
Plasmid#68636PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsCitrineExpressionPlantPromoterMpEF1alpha promoterAvailable SinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
BII-Sh-PnGW
Plasmid#133359PurposePiggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLSDepositorInsertGFP-NLS
TagsHA-tagged GFPExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available SinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB208
Plasmid#68599PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsCitrineExpressionPlantPromoterMpEF1alpha promoterAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only