We narrowed to 7,122 results for: ef1a
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Plasmid#118197PurposeCRISPR-mediated activation of OPTN. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.DepositorInsertdCas9-VP64-2A-BlastR
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a core and U6Available sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiSAMv2 CAMK1D TSS-guide4
Plasmid#118195PurposeCRISPR-mediated activation of CAMK1D. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.DepositorInsertdCas9-VP64-2A-BlastR
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a core and U6Available sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiSAMv2 rs11257655-guide4
Plasmid#118207PurposeCRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs11257655 (CDC123/CAMK1D GWAS locus)DepositorInsertdCas9-VP64-2A-BlastR
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a core and U6Available sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiSAMv2 rs11257655-guide3
Plasmid#118206PurposeCRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs11257655 (CDC123/CAMK1D GWAS locus)DepositorInsertdCas9-VP64-2A-BlastR
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a core and U6Available sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiSAMv2 CAMK1D TSS-guide3
Plasmid#118194PurposeCRISPR-mediated activation of CAMK1D. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.DepositorInsertdCas9-VP64-2A-BlastR
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a core and U6Available sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiSAMv2 CAMK1D TSS-guide1
Plasmid#118192PurposeCRISPR-mediated activation of CAMK1D. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.DepositorInsertdCas9-VP64-2A-BlastR
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a core and U6Available sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiSAMv2 rs7903146-guide3
Plasmid#118204PurposeCRISPR-mediated activation of human islet enhancer containing T2D-associated variant rs7903146 (TCF7L2 GWAS locus)DepositorInsertdCas9-VP64-2A-BlastR
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a core and U6Available sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiSAMv2 EGFP-guide3
Plasmid#118168PurposeCRISPRa negative control. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR, and sgRNA3 against the EGFP CDSDepositorInsertdCas9-VP64-2A-BlastR
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a core and U6Available sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
lentiSAMv2 CAMK1D TSS-guide2
Plasmid#118193PurposeCRISPR-mediated activation of CAMK1D. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.DepositorInsertdCas9-VP64-2A-BlastR
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEF1a core and U6Available sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX_lenti_osTir1_9Myc_P2A_Bsd
Plasmid#139746Purposelentiviral vector expressing rice E3 ligase Tir1 in mammalian cells with selection markerDepositorInsertrice E3 ligase Tir1 (osTir1)
UseTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
mU6-sgRNA-CLTA-mCherry
Plasmid#240637PurposeVector for expressing an sgRNA targeting CLTA promoter from the mouse U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter.DepositorInsertpuro-T2A-mCherry
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEF5B-FRT-control-APEX
Plasmid#73187PurposeExpresses "control"-APEX (cytosolic) in mammalian cellsDepositorInsertcontrol-APEX (Nphp3 Mouse, Glycine max)
UseTagsinternal GFP tagExpressionMammalianMutationchanged glycine 2 to alaninePromoterEF1alphaAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
LLP774_dCas9-Spy-Snoop-Sunx5-Avi-Tag-BFP (dCas9-SSSavi-BFP)
Plasmid#211767PurposedCas9 docking array with four tag domains (Spy, Snoop, aGCN4, Avi), with BFP selectionDepositorInsertdCas9, Spy, Snoop, aGCN4, AviTags
UseTags3xHAExpressionMammalianMutationdCas9 D10A and H840APromoterpGK and pEF1aAvailable sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEF-ABEmax
Plasmid#164415PurposeEF1alpha promoter + ABEmax (derived from #112095). Mammalian cell adenosine base editor expression vector.DepositorInsertABEmax
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterEF1-alphaAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EF1-FHC-POLQ-DM
Plasmid#64880Purposelentiviral expression of human POLQ double mutant (in POL and HDL domains)DepositorInsertPOLQ (POLQ Human)
UseLentiviralTagsFLAG and HAExpressionMammalianMutationK121M and D2330A,Y2331APromoterEF1alphaAvailable sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
Fireworks GFP[PTC-] (AVA2598)
Plasmid#85445PurposeExpresses TEV protease and tandemly-repeated GFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a beta-globin reporter.DepositorInserttDNA1 EF1alfa-5xEGFP-TEVprotease-PEST-beta-globin(dI1)-BGH polyA tDNA2 FRT-PEST-TEVsite-PuromycinR
UseMinimal backbone fragment for low-copy bacterial …TagsExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
Fireworks RFP[PTC-] (AVA2515)
Plasmid#85443PurposeExpresses TEV protease and tandemly-repeated RFP fluorescent proteins to amplify the fluorescence signal produced by a single transcription unit of a beta-globin reporter.DepositorInserttDNA1 EF1alfa-5xtdTomato-TEVprotease-PEST-beta-globin(dI1)-BGH polyA tDNA2 FRT-HygromycinR
UseMinimal backbone fragment for low-copy bacterial …TagsExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
BII-ChBtW-ASCL2
Plasmid#133375PurposePiggybac vector for constitutive expressionDepositorInsertASCL2 (ASCL2 Human)
UseTagsFlagExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-ChiNW-ASCL2
Plasmid#133384PurposePiggybac vector for constitutive expressionDepositorInsertASCL2 (ASCL2 Human)
UseTagsExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-ChPtW-EGFP
Plasmid#133400PurposePiggybac vector for constitutive expression of EGFPDepositorInsertEGFP
UseTagsExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pPV-H1-ccdB-mEF1α-RiH
Plasmid#100598PurposepiggyBac vector for sgRNA cloningDepositorTypeEmpty backboneUsePiggybac vectorTagsExpressionMammalianMutationPromoterHuman H1 PolIII promoter, human FTH1 promoter, mo…Available sinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
betaTrCP mVenus mutant reporter
Plasmid#192459PurposeFluorescent reporter for beta-TrCP with a non-degradable mutation. Used as a control.DepositorInsertCdC25B
UseLentiviralTagsSV40 NLS and mVenusExpressionMammalianMutationaa198-338 of CDC25B. betaTrCP degron sequence (aa…PromoterEF1alphaAvailable sinceOct. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
p1.1-eGFP
Plasmid#162738PurposeFluorescent reporter for CHO cells expression studiesDepositorInsertenhanced green fluorescent protein
UseTagsExpressionMammalianMutationconsensus Kozak sequence (GCCGCCATGG) added befor…PromoterCHO EEF1A1 (Translation Elongation Factor 1 Alpha…Available sinceJuly 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
LLP789_αGCN4-DNMT3A-CO
Plasmid#211783PurposeSunTag counterpart binding domain, aGCN4, fused to DNA methyltransferase DNMT3A, with GFP selectionDepositorInsertaGCN4-DNMT3A
UseTags3xTy1ExpressionMammalianMutationLast 300 bp codon optimised (CO) to detect DNMT3A…PromoterpEF1a and pSV40Available sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVE-FH-IN
Plasmid#70071Purpose2nd gen lentiviral vector. Cre-removable and tet/dox controllable expression of FLAG-HA-tagged transgene in mammalian cellsDepositorTypeEmpty backboneUseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
p1.2-Zeo-eGFP
Plasmid#162743PurposeFluorescent reporter for CHO cells expression studiesDepositorInsertenhanced green fluorescent protein
UseTagsExpressionMammalianMutationconsensus Kozak sequence (GCCGCCATGG) added befor…PromoterCHO EEF1A1 (Translation Elongation Factor 1 Alpha…Available sinceAug. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB321
Plasmid#68649PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsModified EAR motif plant-specific repression doma…ExpressionPlantMutationPromoterMpEF1alpha promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
BII-gR-PtW-eSpCas9
Plasmid#133357PurposePiggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expressionDepositorInserteSpCas9
UseTagsHA-tagged eSpCas9ExpressionMammalianMutationeSpCas9 mutationsPromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB121
Plasmid#68575PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsModified EAR motif plant-specific repression doma…ExpressionPlantMutationPromoterMpEF1alpha promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB221
Plasmid#68612PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsModified EAR motif plant-specific repression doma…ExpressionPlantMutationPromoterMpEF1alpha promoterAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHDR-CDKN2A-Ex2-CMV-H2B-mOrange2
Plasmid#110735PurposeRecombination template for replacing CDKN2A Exon2 with Ef1alpha-H2B-mOrange2DepositorInsertH2B-mOrange2
UseOtherTagsExpressionMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
AI-CN590
Plasmid#62596PurposeKCTD13 N-terminus disruption by homolgy-directed repairDepositorInsertCerulean-P2A-PuroR (KCTD13 Human)
UseTALEN; Homology-directed repair donorTagsExpressionMammalianMutationPromoterhEF1alphaAvailable sinceMarch 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
iSM_PPL2-IP
Plasmid#235989PurposeExpresses iSM_PPL2 in mammalian cellsDepositorInsertiSM_PPL2
UseCRISPRTagsExpressionMammalianMutationreplacement of 5 amino acids of Smac-Cas9 PL2 wit…PromoterEF-1aAvailable sinceAug. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pC2.4
Plasmid#226557PurposeNATURA gene encoding plasmidDepositorInsertNATURA reporter gene
UseLuciferase; PiggybacTagsEGFP and FlagExpressionMammalianMutationPromoterEF1alphaAvailable sinceJan. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
L2_NOP1gRNA-Cas9-CsA
Plasmid#136139PurposePlasmid with NOP1-gRNA to tranform Mp as CRISPR control. Contains Cas9 and HygRDepositorInsertp5-35Sx2:HygR p5-MpU6:NOP1gRNA1 p5-MpEF1a:Cas9-NLS
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB418
Plasmid#68683PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsModified EAR motif plant-specific repression doma…ExpressionPlantMutationPromoterMpEF1alpha promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB421
Plasmid#68686PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsModified EAR motif plant-specific repression doma…ExpressionPlantMutationPromoterMpEF1alpha promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
FH-TET1-pEF
Plasmid#49792PurposeThis plasmid expresses the full-length human TET1 gene with a Flag and HA tag.DepositorInsertTet methylcytosine dioxygenase 1 (TET1 Human)
UseTagsFlag and HAExpressionMammalianMutationPromoterEF-1alphaAvailable sinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-DIO-CFL-SN
Plasmid#181740PurposeAAV vector for expressing CFL-SNDepositorInsertCFL-SN
UseAAVTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB337
Plasmid#68061PurposeGateway-based vector for Marchantia polymorpha to express a floxed gene, with an expression cassette for Cre-glucocorticoid receptor fusion protein under the control of heat-inducible promoterDepositorTypeEmpty backboneUseCre/LoxTagsExpressionPlantMutationPromoterMpEF1alpha promoterAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
p1.2-Hyg-Fur
Plasmid#162774PurposeSoluble human PACE/furin protease expressionDepositorInsertHuman PACE/furin protease soluble (FURIN Human)
UseTagsExpressionMammalianMutationPromoterCHO EEF1A1 (Translation Elongation Factor 1 Alpha…Available sinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWZ403
Plasmid#208113PurposemSwAP-In marker cassette 1 with mScarlet-PuroTKDepositorInsertmScarlet-PuroTK
UseTagsP2AExpressionBacterial and MammalianMutationPromoterEF1alphaAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWZ854
Plasmid#208116PurposemSwAP-In marker cassette 2 with 5’PB-mNeongreen-HPRT1-NeoDepositorInsertmNeonGreen-HPRT1-Neo
UseTagsT2AExpressionBacterial and MammalianMutationPromoterEF1alphaAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWZ852
Plasmid#208114PurposemSwAP-In marker cassette 1 with mScarlet-HygTKDepositorInsertmScarlet-HygTK
UseTagsT2AExpressionBacterial and MammalianMutationPromoterEF1alphaAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJR107
Plasmid#187245PurposeLentiviral sgRNA vector for CROP-seq with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-GFP marker expressionDepositorInsertLentiviral sgRNA vector for CROP-seq with mU6 sgRNA promoter
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJR106
Plasmid#187244PurposeLentiviral sgRNA vector for CROP-seq with mU6 sgRNA promoter, CR1 constant region, and UCOE EF1alpha driving PURO-BFP marker expressionDepositorInsertLentiviral sgRNA vector for CROP-seq with mU6 sgRNA promoter
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
L2_lacZgRNA-Cas9-CsA
Plasmid#136138PurposePlasmid to accept gRNA target with SapI, lacZ blue-white screening. Contains Cas9 and HygR.DepositorInsertp5-35Sx2:HygR p5-MpU6:lacZgRNA p5-MpEF1a:Cas9-NLS
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
FUT8-Dox
Plasmid#124631PurposeDoxycycline-inducible FUT8 expression in mammalian cellsDepositorInsertsAlpha-(1,6)-fucosyltransferase
Tetracycline repressor A3 mutant
TagFP635
UseTagsExpressionMammalianMutationPromoterTRE-tight and hEF1aAvailable sinceJuly 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
BII-ChBtW-IGF2
Plasmid#133374PurposePiggybac vector for constitutive expressionDepositorInsertIGF2 (IGF2 Human)
UseTagsFlagExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only