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Showing: 651 - 700 of 157662 results
  1. pHSN501

    Plasmid
    #50589
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
    Depositor
    Inserts
    zCas9D10A
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG and NLS
    Expression
    Mutation
    Cas9D10A nickase, was derived from the zCas9 and …
    Promoter
    2×35Sp and AtU6-26p
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
  2. pAAV.hSynapsin.SF-iGluSnFR.A184S

    Plasmid
    #106174
    Purpose
    Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Has Service
    AAV1 and AAV9
    Insert
    SF-iGluSnFR.A184S
    Use
    AAV
    Tags
    Expression
    Mutation
    GltI: A184S
    Promoter
    hSynapsin
    Available since
    Feb. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
  3. pAAV.hSynapsin.SF-iGluSnFR.S72A

    Plasmid
    #106176
    Purpose
    Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Has Service
    AAV1
    Insert
    SF-iGluSnFR.S72A
    Use
    AAV
    Tags
    Expression
    Mutation
    GltI: S72A
    Promoter
    hSynapsin
    Available since
    June 19, 2018
    Availability
    Academic Institutions and Nonprofits only
  4. pHSN6A01

    Plasmid
    #50586
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
    Depositor
    Inserts
    dCas9-VP64
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG, HA, and NLS
    Expression
    Mutation
    dCas9 = defective in DNA cleavage; 4×minimal VP16…
    Promoter
    2×35Sp and AtU6-26p
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
  5. pAAV.hSynapsin.SF-Venus-iGluSnFR.A184V

    Plasmid
    #106178
    Purpose
    Medium affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Insert
    SF-Venus-iGluSnFR.A184V
    Use
    AAV
    Tags
    Expression
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: A184V
    Promoter
    hSynapsin
    Available since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
  6. pHSN6I01

    Plasmid
    #50587
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-KRAB, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
    Depositor
    Inserts
    dCas9-KRAB
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG, HA, and NLS
    Expression
    Mutation
    dCas9 that is defective in DNA cleavage; the maiz…
    Promoter
    2×35Sp and AtU6-26p
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
  7. pBUN6A11

    Plasmid
    #50579
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-VP64, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance
    Depositor
    Inserts
    dCas9-VP64
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG, HA, and NLS
    Expression
    Mutation
    dCas9 = defective in DNA cleavage; 4×minimal VP16…
    Promoter
    OsU3p and Ubi1p
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
  8. pBUN6I11

    Plasmid
    #50580
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses dCas9-KRAB, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance
    Depositor
    Inserts
    dCas9-KRAB
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG, HA, and NLS
    Expression
    Mutation
    dCas9 that is defective in DNA cleavage; the maiz…
    Promoter
    OsU3p and Ubi1p
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
  9. pAAV.hSynap-FLEX.SF-iGluSnFR.A184V

    Plasmid
    #106181
    Purpose
    Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Has Service
    AAV1
    Insert
    SF-iGluSnFR.A184V
    Use
    AAV
    Tags
    Expression
    Mutation
    GltI: A184V
    Promoter
    hSynapsin
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
  10. pAAV.CAG.SF-Venus-iGluSnFR.A184S

    Plasmid
    #106201
    Purpose
    Tight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Insert
    SF-Venus-iGluSnFR.A184S
    Use
    AAV
    Tags
    Expression
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: A184S
    Promoter
    CAG
    Available since
    Feb. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
  11. pAAV.CAG-FLEX.SF-iGluSnFR.A184S.mRuby3

    Plasmid
    #106204
    Purpose
    Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Insert
    SF-iGluSnFR.A184S
    Use
    AAV
    Tags
    Expression
    Mutation
    GltI: A184S + mRuby3 fusion
    Promoter
    CAG
    Available since
    Feb. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
  12. pAAV.hSynapsin.SF-Venus-iGluSnFR.A184S

    Plasmid
    #106177
    Purpose
    Tight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Insert
    SF-Venus-iGluSnFR.A184S
    Use
    AAV
    Tags
    Expression
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: A184S
    Promoter
    hSynapsin
    Available since
    Feb. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
  13. pAAV.CAG-FLEX.SF-iGluSnFR.A184V.mRuby3

    Plasmid
    #106205
    Purpose
    Medium affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Insert
    SF-iGluSnFR.A184V
    Use
    AAV
    Tags
    Expression
    Mutation
    GltI: A184V + mRuby3 fusion
    Promoter
    CAG
    Available since
    Feb. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
  14. pBUN501

    Plasmid
    #50582
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses zCas9D10A, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Bar resistance
    Depositor
    Inserts
    zCas9D10A
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG and NLS
    Expression
    Mutation
    Cas9D10A nickase, was derived from the zCas9 and …
    Promoter
    AtU6-26p and Ubi1p
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
  15. pAAV.CAG-FLEX.SF-iGluSnFR.S72A.mRuby3

    Plasmid
    #106206
    Purpose
    Weak affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Insert
    SF-iGluSnFR.S72A
    Use
    AAV
    Tags
    Expression
    Mutation
    GltI: S72A + mRuby3 fusion
    Promoter
    CAG
    Available since
    April 17, 2018
    Availability
    Academic Institutions and Nonprofits only
  16. pAAV.CAG.SF-Venus-iGluSnFR.A184V

    Plasmid
    #106202
    Purpose
    Medium affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Insert
    SF-Venus-iGluSnFR.A184V
    Use
    AAV
    Tags
    Expression
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: A184V
    Promoter
    CAG
    Available since
    Feb. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
  17. pAAV.CAG.SF-Venus-iGluSnFR.S72A

    Plasmid
    #106203
    Purpose
    Weak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Insert
    SF-Venus-iGluSnFR.S72A
    Use
    AAV
    Tags
    Expression
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: S72A
    Promoter
    CAG
    Available since
    April 17, 2018
    Availability
    Academic Institutions and Nonprofits only
  18. pAAV.GFAP.SF-Venus-iGluSnFR.A184S

    Plasmid
    #106195
    Purpose
    Tight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Insert
    SF-Venus-iGluSnFR.A184S
    Use
    AAV
    Tags
    Expression
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: A184S
    Promoter
    GFAP
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
  19. pAAV.hSynapsin.SF-Venus-iGluSnFR.S72A

    Plasmid
    #106179
    Purpose
    Weak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Insert
    SF-Venus-iGluSnFR.S72A
    Use
    AAV
    Tags
    Expression
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: S72A
    Promoter
    hSynapsin
    Available since
    April 17, 2018
    Availability
    Academic Institutions and Nonprofits only
  20. G30, Cdc20-mCherry-AID tagging

    Plasmid
    #130270
    Purpose
    Tagging the Saccharomyces cerevisiae Cdc20 gene with a mCherry and auxin-degron tag, via homologous recombination
    Depositor
    Insert
    Cdc20/mCherry-AID(71-114)-tCYC1-NatMX (CDC20 Budding Yeast, Synthetic)
    Use
    Tags
    AID(71-114) and mCherry
    Expression
    Yeast
    Mutation
    Promoter
    Native promoter
    Available since
    Sept. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
  21. pAAV.GFAP.SF-Venus-iGluSnFR.A184V

    Plasmid
    #106196
    Purpose
    Medium affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Insert
    SF-Venus-iGluSnFR.A184V
    Use
    AAV
    Tags
    Expression
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: A184V
    Promoter
    GFAP
    Available since
    Feb. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
  22. pAAV.GFAP.SF-Venus-iGluSnFR.S72A

    Plasmid
    #106197
    Purpose
    Weak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Insert
    SF-Venus-iGluSnFR.S72A
    Use
    AAV
    Tags
    Expression
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: S72A
    Promoter
    GFAP
    Available since
    April 17, 2018
    Availability
    Academic Institutions and Nonprofits only
  23. pAAV.CAG-FLEX.SF-Venus-iGluSnFR.A184S

    Plasmid
    #106189
    Purpose
    Tight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Insert
    SF-Venus-iGluSnFR.A184S
    Use
    AAV
    Tags
    Expression
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: A184S
    Promoter
    CAG
    Available since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
  24. pAAV.hSynap-FLEX.SF-Venus-iGluSnFR.A184S

    Plasmid
    #106183
    Purpose
    Tight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Has Service
    AAV1
    Insert
    SF-Venus-iGluSnFR.A184S
    Use
    AAV
    Tags
    Expression
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: A184S
    Promoter
    hSynapsin
    Available since
    July 5, 2018
    Availability
    Academic Institutions and Nonprofits only
  25. pAAV.CAG-FLEX.SF-Venus-iGluSnFR.A184V

    Plasmid
    #106190
    Purpose
    Medium affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Insert
    SF-Venus-iGluSnFR.A184V
    Use
    AAV
    Tags
    Expression
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: A184V
    Promoter
    CAG
    Available since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
  26. pAAV.hSynap-FLEX.SF-Venus-iGluSnFR.S72A

    Plasmid
    #106185
    Purpose
    Weak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Has Service
    AAV1
    Insert
    SF-Venus-iGluSnFR.S72A
    Use
    AAV
    Tags
    Expression
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: S72A
    Promoter
    hSynapsin
    Available since
    April 17, 2018
    Availability
    Academic Institutions and Nonprofits only
  27. pAAV.CAG-FLEX.SF-Venus-iGluSnFR.S72A

    Plasmid
    #106191
    Purpose
    Weak affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Insert
    SF-Venus-iGluSnFR.S72A
    Use
    AAV
    Tags
    Expression
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: S72A
    Promoter
    CAG
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
  28. pAAV.hSynap-FLEX.SF-Venus-iGluSnFR.A184V

    Plasmid
    #106184
    Purpose
    Medium affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Insert
    SF-Venus-iGluSnFR.A184V
    Use
    AAV
    Tags
    Expression
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: A184V
    Promoter
    hSynapsin
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
  29. pCMV(MinDis).iGluSnFR

    Plasmid
    #41732
    Purpose
    Single-wavelength extracellular glutamate sensor constructed from E. coli Gltl and cpGFP. Membrane-displayed. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Insert
    GltI based Glutamate Biosensor
    Use
    Glutamate biosensor
    Tags
    Myc, PDGFR transmembrane helix (aa 513-561), and …
    Expression
    Mammalian
    Mutation
    cpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…
    Promoter
    CMV
    Available since
    Jan. 3, 2013
    Availability
    Academic Institutions and Nonprofits only
  30. pRS416-dCas9-Mxi1 + TetR + pRPR1(TetO)-NotI-gRNA

    Plasmid
    #73796
    Purpose
    pRS416 Ura marked Cen/Ars plasmid with dCas9-Mxi1 under Tef1 promoter, and tet-incucibile RPR1 promoter with NotI cloning site adjacent to gRNA
    Depositor
    Inserts
    dCas9-Mxi1
    Tet Repressor
    Structural gRNA for S pyogenes
    Use
    CRISPR
    Tags
    Mxi1 and NLS
    Expression
    Yeast
    Mutation
    D10A, H840A
    Promoter
    pGPM1, pRPR1(TetO), and pTef1
    Available since
    March 10, 2016
    Availability
    Academic Institutions and Nonprofits only
  31. pABD1031

    Plasmid
    #68004
    Purpose
    Protein co-localization of PIF1 (PIL5) via fluorescent fusion after transient expression in planta
    Depositor
    Insert
    PHY-INTERACTING FACTOR 1 (PIF1) (PIL5 )
    Use
    Tags
    eYFP
    Expression
    Plant
    Mutation
    Promoter
    2X Cauliflower Mosaic Virus 35S promoter
    Available since
    Dec. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
  32. pGGG-Sr43

    Plasmid
    #186974
    Purpose
    Sr43 is a wheat stem rust resistance gene transferred from tall wheat grass (Thinopyrum ponticum) into bread wheat (Triticum aestivum).
    Depositor
    Insert
    Sr43
    Use
    Tags
    None
    Expression
    Bacterial and Plant
    Mutation
    None
    Promoter
    Natice
    Available since
    April 4, 2023
    Availability
    Academic Institutions and Nonprofits only
  33. pRK7

    Plasmid
    #10883
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 13, 2006
    Availability
    Academic Institutions and Nonprofits only
  34. 401 pSG5L

    Plasmid
    #10737
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 27, 2005
    Availability
    Academic Institutions and Nonprofits only
Showing: 651 - 700 of 157662 results