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pSico shSMYD3-C human
Plasmid#85654Purposeexpression of shRNA targeting human SMYD3DepositorInsertshRNA targeting 3'UTR of SMYD3 (SMYD3 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterU6Available sinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
myc-Med4 pBacPAK8
Plasmid#15364DepositorInsertMed4 (MED4 N/A, Human)
UseBaculovirus constructionTagsFLAGExpressionMutationPromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
PHB2 S39A-mCherry
Plasmid#232786PurposeEncodes an mCherry-tagged Prohibitin2/PHB2 non-phosphorylatable on Ser39DepositorInsertPHB2 (PHB2 Human)
UseTagsmCherryExpressionMammalianMutationChanged Ser 39 to AlaPromoterCMVAvailable sinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
PHB2 S39D-mCherry
Plasmid#232787PurposeEncodes an mCherry-tagged Prohibitin2/PHB2 constitutively phosphorylated on Ser39DepositorInsertPHB2 (PHB2 Human)
UseTagsmCherryExpressionMammalianMutationChanged Serine 39 to AspartatePromoterCMVAvailable sinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
tetO-NFYB
Plasmid#170697Purposedoxycycline-inducible overexpression of NFYBDepositorInsertNYFB (NFYB Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationPromoterTRE promoter, Tet-ONAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1126
Plasmid#142245PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTHAP3 (THAP3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF1128
Plasmid#141798PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTHAP3 (THAP3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
FKBP-beta3(702-1094)-GFP
Plasmid#100734PurposeTo express a clathrin "hook" in mammalian cells to hot-wire endocytosisDepositorInsertAP3B1 (AP3B1 Human)
UseTagsFKBP and GFPExpressionMammalianMutationappendage and ear onlyPromoterCMVAvailable sinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
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FLAG-MED27
Plasmid#15424DepositorInsertMED27 (MED27 Human)
UseTagsFLAGExpressionMammalianMutationN/APromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCAG-SRGAP2A-FBAR-mRFP
Plasmid#168502PurposeExpression of the F-BAR domain (aa1-501) of SRGAP2 with a C-terminal mRFP fusion.DepositorInsertSRGAP2A-FBAR (SRGAP2 Human)
UseTagsmRFPExpressionMammalianMutationPromoterCAGAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-mCGN-mCGNL1-Rod1-Myc
Plasmid#236526PurposeFor expression of chimeric CGN containing the Rod1 domain from CGNL1 (mouse) in mammalian cellsDepositorInsertCGN-CGNL1 (Cgn Mouse)
UseTagsGFP and MycExpressionMammalianMutationWTPromoterCMVAvailable sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAG_SRGAP2A_SH3(W765A)_GFP
Plasmid#222616PurposeExpresses a form of full length SRGAP2 with one point mutation (W765A) in the SH3 domain abrogating its ability to bind to Gephyrin.DepositorInsertSRGAP2 (SRGAP2 Human)
UseTagsEGFPExpressionMammalianMutationPoint mutation W765A (SH3 domain)PromoterAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG_SRGAP2A_SH3(W765A)_HA
Plasmid#222614PurposeExpresses a form of full length SRGAP2 with one point mutation (W765A) in the SH3 domain abrogating its ability to bind to Gephyrin.DepositorInsertSRGAP2 (SRGAP2 Human)
UseTagsHAExpressionMammalianMutationPoint mutation W765A (SH3 domain)PromoterAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG SRGAP2A_EVH1(P339L F342C)
Plasmid#222613PurposeExpresses a form of full length SRGAP2 with two point mutations (P339L and F342C) that abrogate EVH1 binding site for Homer1DepositorInsertSRGAP2 (SRGAP2 Human)
UseTagsEGFPExpressionMammalianMutationPoint mutations P339L and F342CPromoterAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SRGAP2-1/1
Plasmid#91263PurposeProtein expression and purification of human SH3 domain construct SRGAP2-1/1DepositorInsertSRGAP2-1/1 (SRGAP2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCJ136
Plasmid#162665PurposepET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag.DepositorInsertMHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
UseTagsHisExpressionBacterialMutationcodon optimized for expression in E. coli K12PromoterT7/lacAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG_SRGAP2A_GAP(R527L_K566A)
Plasmid#222617PurposeExpresses full length SRGAP2 with R527L, K566A mt in the Rac1-specific GAP domain that abrogates the GTPase activating function of SRGAP2 without locking the GAP-domain in a form bound to Rac1-GDP.DepositorInsertSRGAP2 (SRGAP2 Human)
UseTagsEGFPExpressionMammalianMutationPoint mutations R527L and K566A (Rac1-specific GA…PromoterAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
M3K5_HUMAN_D0
Plasmid#79710PurposeThis plasmid encodes the kinase domain of M3K5. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorInsertM3K5 (MAP3K5 Human)
UseTagsHis10-TEVExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only
