We narrowed to 22,296 results for: ESC;

1. pGL3-Ctr2-gRNA-EGFP

   Plasmid

   #226001

   Purpose

   Negative control for CRISPRi-KD

   Depositor

   Miguel Ramalho-Santos

   Article

   Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

   Insert

   negtive control sgRNA of no specific targets in human genome
   

   Expression

   Mammalian

   Available Since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL3-hL1-gRNA1-EGFP

   Plasmid

   #226002

   Purpose

   CRISPRi-KD of human LINE1

   Depositor

   Miguel Ramalho-Santos

   Article

   Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

   Insert

   sgRNA targeting human L1HS
   

   Expression

   Mammalian

   Available Since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL3-hL1-gRNA2-EGFP

   Plasmid

   #226003

   Purpose

   CRISPRi-KD of human LINE1

   Depositor

   Miguel Ramalho-Santos

   Article

   Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

   Insert

   sgRNA targeting human L1HS
   

   Expression

   Mammalian

   Available Since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. miR290-SE-RGM-tdTomato targeting vector

   Plasmid

   #133047

   Purpose

   Targeting vector of RGM-tdTomato with homologous arms around Mir290 super-enhancer DMR

   Depositor

   Rudolf Jaenisch

   Article

   Song et al Mol Cell. 2019 Aug 15. pii: S1097-2765(1

   Insert

   miR290-SE-5arm-RGM-miR290-SE-3arm
   

   Use

   Mouse Targeting

   Available Since

   Oct. 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNR2E3-donor

   Plasmid

   #176369

   Purpose

   CRISPR donor plasmid to create GFP fusion proteins

   Depositor

   Barbara Stranger, Kevin White

   Insert

   NR2E3 (NR2E3 Human)
   

   Use

   CRISPR

   Tags

   GFP

   Available Since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pT2-hUbC-dCas9-KRAB-T2a-Puro

   Plasmid

   #225999

   Purpose

   To establish stable dCas9-KRAB knock-in line

   Depositor

   Miguel Ramalho-Santos

   Article

   Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

   Insert

   humanized dCas9-KRAB T2A Puro
   

   Use

   Sleeping beauty transposon

   Expression

   Mammalian

   Mutation

   D10A and H840A

   Available Since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. OCT4 GFP puro Donor 1

   Plasmid

   #22209

   Depositor

   Rudolf Jaenisch

   Article

   Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

   Insert

   SA-OCT-GFP-2APuro-PA (POU5F1 Human)
   

   Available Since

   Oct. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. TV-hPITX3-forward

   Plasmid

   #22073

   Depositor

   Rudolf Jaenisch

   Article

   Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

   Insert

   PITX3 (ASMD Human)
   

   Use

   Cre/Lox; Zfn donor

   Tags

   eGFP

   Available Since

   Oct. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. OCT4 GFP puro Donor 2

   Plasmid

   #22210

   Depositor

   Rudolf Jaenisch

   Article

   Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

   Insert

   SA-OCT-GFP-2APuro-PA (POU5F1 Human)
   

   Available Since

   Oct. 14, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. OCT4 GFP puro Donor 3

    Plasmid

    #22211

    Depositor

    Rudolf Jaenisch

    Article

    Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

    Insert

    SA-OCT-GFP-2APuro-PA (POU5F1 Human)
    

    Available Since

    Oct. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGL2-hOct4 DE-SV40-Luc

    Plasmid

    #26279

    Purpose

    Addgene next-generation sequencing identified numerous modifications from the indicated plasmid backbone. Please see the sequences link for additional information.

    Depositor

    Rudolf Jaenisch

    Article

    Hanna et al Proc Natl Acad Sci U S A. 2010 May 18. 1

    Insert

    Oct4 Distal Enhancer Element (DE) (POU5F1 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    See Depositor Comments below

    Available Since

    Oct. 18, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. pU6-hL1-5'_3.3kb plasmid

    Plasmid

    #226006

    Purpose

    Plasmid used to generate DNA-FISH probe targeting at human L1HS

    Depositor

    Miguel Ramalho-Santos

    Article

    Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

    Insert

    Human L1 element 3.3kb sequences at the 5' end
    

    Use

    Synthetic Biology

    Available Since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL3-NCL-gRNA1-EGFP

    Plasmid

    #226004

    Purpose

    CRISPRi-KD of human Nucleolin

    Depositor

    Miguel Ramalho-Santos

    Article

    Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

    Insert

    sgRNA targeting human Nucleolin (NCL Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGL3-NCL-gRNA2-EGFP

    Plasmid

    #226005

    Purpose

    CRISPRi-KD of human Nucleolin

    Depositor

    Miguel Ramalho-Santos

    Article

    Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

    Insert

    sgRNA targeting human Nucleolin (NCL Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-SU(Z)12(624-900)-H6

    Plasmid

    #183772

    Purpose

    Express of SU(Z)12(624-900)-H6 in E. coli. Purified SU(Z)12(624-900)-H6 was used for raising polyclonal anti-SU(Z)12 antibody in guinea pigs.

    Depositor

    Peter Harte

    Article

    Tie et al Mol Cell Biol. 2003 May;23(9):3352-62. d

    Insert

    SU(Z)12 residues 624-900 (Su(z)12 Fly)
    

    Tags

    His tag at the C-terminus

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. TV-hPITX3-Reverse

    Plasmid

    #22208

    Depositor

    Rudolf Jaenisch

    Article

    Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

    Insert

    PITX3-eGFP-loxP-PGK-Puro-loxP (PITX3, PGK1 Human)
    

    Use

    Zfn donor

    Available Since

    Oct. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCIP

    Plasmid

    #79009

    Purpose

    For expression in mouse ESCs or other eukaryotic cells. Contains pCAGGS-IRES-Puro-bpA, with a NheI-MfeI cloning site in between pCAGGS and IRES.

    Depositor

    Reuben Shaw

    Article

    Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    pCAGGs

    Available Since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHH0103_NR2E3_Hormone-recep

    Plasmid

    #109852

    Purpose

    Protein expression and purification of NR2E3_Hormone-recep

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    NR2E3_Hormone-recep (NR2E3 Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330_gRNA_IG-DMR-1

    Plasmid

    #159931

    Purpose

    Specific gRNA against mouse IG-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of IG-DMR in mouse ESC.

    Depositor

    Anton Wutz

    Article

    Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

    Insert

    gRNA mouse IG-DMR
    

    Use

    CRISPR and Mouse Targeting

    Expression

    Mammalian

    Promoter

    U6, CBh

    Available Since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX330_gRNA_IG-DMR-2

    Plasmid

    #159932

    Purpose

    Specific gRNA against mouse IG-DMR cloned in the pX330 backbone (Addgene Number 42230). Deletion of IG-DMR in mouse ESC.

    Depositor

    Anton Wutz

    Article

    Aizawa et al PLoS One. 2020 Sep 10;15(9):e0233072. do

    Insert

    gRNA mouse IG-DMR
    

    Use

    CRISPR and Mouse Targeting

    Expression

    Mammalian

    Promoter

    U6, CBh

    Available Since

    Nov. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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