We narrowed to 7,125 results for: RAP

1. pMT Draper-GFP

   Plasmid

   #126653

   Purpose

   Inducible expression of Draper-GFP in S2 cells

   Depositor

   Ron Vale

   Article

   Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

   Insert

   Draper
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRK5-HA-YFP-Raptor K840R

   Plasmid

   #204418

   Purpose

   Acetylation mutant for human raptor

   Depositor

   David Rubinsztein

   Article

   Son et al Cell Metab. 2019 Jan 8;29(1):192-201.e7.

   Insert

   Regulatory Associated Protein Of MTOR, Complex 1
   

   Use

   Tags

   HA and YFP

   Expression

   Mammalian

   Mutation

   Lysine 840 to Arginine (K840R)

   Promoter

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRK5-HA-YFP-Raptor K1097R

   Plasmid

   #204417

   Purpose

   Acetylation mutant for human raptor

   Depositor

   David Rubinsztein

   Article

   Son et al Cell Metab. 2019 Jan 8;29(1):192-201.e7.

   Insert

   Regulatory Associated Protein Of MTOR, Complex 1
   

   Use

   Tags

   HA and YFP

   Expression

   Mammalian

   Mutation

   Lysine 1097 to Arginine (K1097R)

   Promoter

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pWZL hygro Flag HA TRAP220 wt

   Plasmid

   #17433

   Purpose

   Retroviral expression of Med1/TRAP220 in mammalian cells

   Depositor

   Kai Ge

   Article

   Ge et al Mol Cell Biol. 2008 Feb . 28(3):1081-91.

   Insert

   TRAP220 (MED1 Human)
   

   Use

   Retroviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-PGK-RAP2A-WT-neo

   Plasmid

   #156177

   Purpose

   Lentiviral vector for expression of RAP2A-WT, with neomycin selection

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   RAP2A (RAP2A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   wild type form

   Promoter

   human PGK

   Available since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLJM1 Flag Raptor Rheb15

   Plasmid

   #26634

   Depositor

   David Sabatini

   Article

   Sancak et al Cell. 2010 Apr 16. 141(2):290-303.

   Insert

   raptor-Rheb15 (RPTOR Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMT2-HA-RAPGEF2(S1244A/S1248A)

   Plasmid

   #110163

   Purpose

   Expression of human RAPGEF2 (S1244A/S1248A) in mammalian cells

   Depositor

   Daniele Guardavaccaro

   Article

   Magliozzi et al Dev Cell. 2013 Dec 9;27(5):574-85. doi:

   Insert

   RAPGEF2 (Rap guanine nucleotide exchange factor 2 (RAPGEF2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   S1244A/S1248A

   Promoter

   CMV

   Available since

   June 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-PGK-RAP1A-WT-puro

   Plasmid

   #156166

   Purpose

   Lentiviral vector for expression of RAP1A-WT, with puromycin selection

   Depositor

   Roland Friedel

   Article

   Junqueira Alves et al Nat Commun. 2021 Oct 14;12(1):6019. doi:

   Insert

   RAP1A (RAP1A Human)
   

   Use

   Lentiviral

   Tags

   none

   Expression

   Mammalian

   Mutation

   wild type form

   Promoter

   human PGK

   Available since

   July 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pProEx Rap1A(G12C, L96F) CL

   Plasmid

   #224264

   Purpose

   Protein purification of Rap1A (G12C, L96F)

   Depositor

   Kevan Shokat

   Article

   Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

   Insert

   Rap1A (RAP1A Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   G12C, L96F

   Promoter

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. AIRAP 9: PSMD2.GST.V2.pcDNA3

    Plasmid

    #21799

    Depositor

    David Ron

    Article

    Stanhill et al Mol Cell. 2006 Sep 15. 23(6):875-85.

    Insert

    PSMD2 (PSMD2 Human)
    

    Use

    Tags

    GST

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO mouse shRNA 1 raptor

    Plasmid

    #21339

    Depositor

    David Sabatini

    Article

    Thoreen et al J Biol Chem. 2009 Mar 20. 284(12):8023-3

    Insert

    raptor shRNA 1 (Rptor Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 7, 2009

    Availability

    Academic Institutions and Nonprofits only

12. pAXEF-Rap1-E63(GTP)

    Plasmid

    #32698

    Depositor

    Vicki Boussiotis

    Article

    Lafuente et al Dev Cell. 2004 Oct;7(4):585-95.

    Insert

    RAP1 (RAP1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E63: Consitutively GTP-bound

    Promoter

    EF promoter

    Available since

    Dec. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUSE-RapR-Src-myc

    Plasmid

    #25933

    Depositor

    Klaus Hahn

    Article

    Karginov et al Nat Biotechnol. 2010 Jun 27. ():.

    Insert

    RapR-Src (Src Mouse)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 9, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDEST-CMV GFP-GABARAPL1 WT

    Plasmid

    #123117

    Purpose

    Expresses EGFP-GABARAPL1 wild-type in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1-MYO10-HMM-Nanotrap

    Plasmid

    #87255

    Purpose

    Expression plasmid to perform NanoSPD 2.0 assays in mammalian cells.

    Depositor

    Thomas Friedman

    Article

    Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

    Insert

    MYO10 (Myo10 Mouse)
    

    Use

    Tags

    GFP-nanotrap

    Expression

    Mammalian

    Mutation

    Myo10 is truncated to contain aa.1-941

    Promoter

    CMV

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pUSE-RapR-Src(KD)-myc

    Plasmid

    #25934

    Depositor

    Klaus Hahn

    Article

    Karginov et al Nat Biotechnol. 2010 Jun 27. ():.

    Insert

    RapR-Src (Fkbp1a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    This pUSE-RapR-Src(KD)-myc plasmid contains the k…

    Promoter

    Available since

    Oct. 26, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLKO mouse shRNA 2 raptor

    Plasmid

    #21340

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2009 May 13. ():.

    Insert

    raptor shRNA 2 (Rptor Mouse)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 6, 2009

    Availability

    Academic Institutions and Nonprofits only

18. pNAS1B split mCherry N-ME(pS)VD/TRAP(RK)-C

    Plasmid

    #61966

    Purpose

    Positive control duet plasmid for SepOTS-dependent split mCherry assembly assay (i.e., N-mCherry is fused to the peptide ME(pS)VD (pS = phosphoserine); C-mCherry is fused to TRAP(RK))

    Depositor

    Lynne Regan

    Article

    Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.

    Inserts

    N-mCherry-ME(pS)VD
    TRAP(RK)-C-mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD and PLtetO

    Available since

    Feb. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMT Draper-Ala11-GFP

    Plasmid

    #126654

    Purpose

    Inducible expression of Draper-Ala11-GFP mutant in S2 cells

    Depositor

    Ron Vale

    Article

    Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

    Insert

    Draper cytoplasmi Tyr residues mutated to Ala
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Cytoplasmic Tyr residues to Ala

    Promoter

    Available since

    Dec. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBactin-td-mCherry-Cdk5rap2-CTD

    Plasmid

    #196854

    Purpose

    Expression of the centrosome targeting carboxy-terminal domain (CTD) of Cdk5rap2 fused to tandem (td)-mCherry. Used to displace endogenous Cdk5rap2 from centrosomes.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    td-mCherry-Cdk5rap2-CTD (Cdk5rap2 Discosoma sp.)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    None (wt)

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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