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We narrowed to 5,501 results for: golden gate

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Showing: 261 - 280 of 5501 results

  1. pICSL01005_CP

    Plasmid
    #245689
    Purpose
    Level 0 Golden Gate Coding Sequence (WITHOUT stop codon) acceptor with AmilCP negative selection cassette AATG - TTCG
    Depositor
    Mark Youles
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available since
    Dec. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    245689-plasmid-map-sequence-id-495758

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  2. pICSL60027

    Plasmid
    #245675
    Purpose
    Level 0 Golden Gate part Extensin-Truncated-EU + NbACT + Rb7MAR (D144-437 variant) terminator, GCTT - CGCT
    Depositor
    Mark Youles
    Insert
    Extensin-Truncated-EU + NbACT + Rb7MAR (D144-437 variant) terminator
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245675-plasmid-map-sequence-id-495497

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  4. pDOC-GG

    Plasmid
    #149377
    Purpose
    Chassis plasmid for building mutagenesis cassettes by Golden Gate assembly to form a donor plasmid for recombineering by Gene Doctoring
    Depositor
    Mark Pallen
    Publication
    Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi
    Inserts
    Fragment containing a kanamycin resistance cassette
    Fragment containing pMB1 origin of replication and an I-SceI recognition sequence
    Fragment containing a lacZ⍺ expression cassette flanked by BsaI sites
    Fragment containing an I-SceI recognition sequence
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    149377-plasmid-map-sequence-id-308794

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  5. pICSL01005-LC

    Plasmid
    #245697
    Purpose
    Level 0 Golden Gate Coding Sequence (WITHOUT stop codon) acceptor, Low - copy with pBR322 origin of replication AATG - GCTT
    Depositor
    Mark Youles
    Type
    Empty backbone
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available since
    Dec. 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    245697-plasmid-map-sequence-id-495759

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  6. pICSL12047

    Plasmid
    #245644
    Purpose
    Level 0 Golden Gate part SGT-34S FMV (Figwort Mosaic Virus). Sub-Genomic Transcript (SGT) promoter, GGAG - AATG
    Depositor
    Mark Youles
    Insert
    SGT-34S FMV (Figwort Mosaic Virus). Sub-Genomic Transcript (SGT) promoter
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245644-plasmid-map-sequence-id-495540

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  7. pCB1532B-RFP

    Plasmid
    #101854
    Purpose
    Golden-Gate compatible fungal transformation vector carrying BASTA (Bialaphos/ Phosphinothricin) Resistance w RFP screening cassette (RFP knocked out by GG reaction, allowing for red-white screening)
    Depositor
    Sophien Kamoun
    Publication
    Pennington et al Figshare (2017)
    Type
    Empty backbone
    Use
    Fungal expression plasmid, tested in magnaporthe
    Available since
    March 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    101854-plasmid-map-sequence-id-204750

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  8. pL1P4 ZmUbiP:GRF-GIF:NosT

    Plasmid
    #198048
    Purpose
    MoClo Golden Gate Level 1 Position 4. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.
    Depositor
    Cristobal Uauy, John Innes Centre - Wheat Transformation and Genome Editing
    Insert
    ZmUbiP::TaGRF4-GIF1::NosT
    Use
    Synthetic Biology
    Promoter
    Zea mays (maize) ubiquitin promoter
    Available since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    198048-plasmid-map-sequence-id-389382

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  9. pL1P5 ZmUbiP:GRF-GIF:NosT

    Plasmid
    #198046
    Purpose
    MoClo Golden Gate Level 1 Position 5. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.
    Depositor
    Cristobal Uauy, John Innes Centre - Wheat Transformation and Genome Editing
    Insert
    ZmUbiP::TaGRF4-GIF1::NosT
    Use
    Synthetic Biology
    Promoter
    Zea mays (maize) ubiquitin promoter
    Available since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    198046-plasmid-map-sequence-id-383687

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  11. pICSL13008

    Plasmid
    #245656
    Purpose
    Level 0 Golden Gate part Long 35s gene cauliflower mosaic virus and 5 prime untranslated leader tobacco mosaic virus promoter, GGAG - CCAT
    Depositor
    Mark Youles
    Insert
    Long 35s gene cauliflower mosaic virus and 5 prime untranslated leader tobacco mosaic virus promoter
    Use
    Synthetic Biology
    Mutation
    BsaI/BpiI sites removed by point-mutation
    Available since
    Dec. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    245656-plasmid-map-sequence-id-495550

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  12. pL1P3 ZmUbiP:GRF-GIF:NosT

    Plasmid
    #198047
    Purpose
    MoClo Golden Gate Level 1 Position 3. Overexpression cassette of Growth-Regulating Factor 4 (GRF4) plus GRF-Interacting Factor 1 (GRF4-GIF1). Improves in vitro wheat regeneration and transformation.
    Depositor
    Cristobal Uauy, John Innes Centre - Wheat Transformation and Genome Editing
    Insert
    ZmUbiP::TaGRF4-GIF1::NosT
    Use
    Synthetic Biology
    Promoter
    Zea mays (maize) ubiquitin promoter
    Available since
    March 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    198047-plasmid-map-sequence-id-389251

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  15. pCORE_13__pBR322_KanR_LacZ

    Plasmid
    #261308
    Purpose
    This plasmid can fuction as Moclo Level 1 (BsaI) or Level 2 (BpiI) acceptors and as starting backbones for developing vectors for other hosts through AarI-mediated Golden Gate assembly.
    Depositor
    Karen Sarkisyan
    Type
    Empty backbone
    Use
    Other
    Available since
    Sept. 18, 2026
    Availability
    Academic Institutions and Nonprofits only
    261308-plasmid-map-sequence-id-521401

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  16. pCORE_33__pSC101_KanR_LacZ

    Plasmid
    #261315
    Purpose
    This plasmid can fuction as Moclo Level 1 (BsaI) or Level 2 (BpiI) acceptors and as starting backbones for developing vectors for other hosts through AarI-mediated Golden Gate assembly.
    Depositor
    Karen Sarkisyan
    Type
    Empty backbone
    Use
    Other
    Available since
    Sept. 18, 2026
    Availability
    Academic Institutions and Nonprofits only
    261315-plasmid-map-sequence-id-521400

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  17. pCORE_18__pBR322_KanR_mScarlet

    Plasmid
    #261321
    Purpose
    This plasmid can fuction as Moclo Level 1 (BsaI) or Level 2 (BpiI) acceptors and as starting backbones for developing vectors for other hosts through AarI-mediated Golden Gate assembly.
    Depositor
    Karen Sarkisyan
    Type
    Empty backbone
    Use
    Other
    Available since
    Sept. 18, 2026
    Availability
    Academic Institutions and Nonprofits only
    261321-plasmid-map-sequence-id-521399

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  18. pCORE_38__pSC101_KanR_mScarlet

    Plasmid
    #261327
    Purpose
    This plasmid can fuction as Moclo Level 1 (BsaI) or Level 2 (BpiI) acceptors and as starting backbones for developing vectors for other hosts through AarI-mediated Golden Gate assembly.
    Depositor
    Karen Sarkisyan
    Type
    Empty backbone
    Use
    Other
    Available since
    Sept. 18, 2026
    Availability
    Academic Institutions and Nonprofits only
    261327-plasmid-map-sequence-id-521402

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  19. pCORE_15__pBR322_ZeoR_LacZ

    Plasmid
    #261310
    Purpose
    This plasmid can fuction as Moclo Level 1 (BsaI) or Level 2 (BpiI) acceptors and as starting backbones for developing vectors for other hosts through AarI-mediated Golden Gate assembly.
    Depositor
    Karen Sarkisyan
    Type
    Empty backbone
    Use
    Other
    Available since
    Sept. 18, 2026
    Availability
    Academic Institutions and Nonprofits only
    261310-plasmid-map-sequence-id-521398

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  20. pCORE_20__pBR322_ZeoR_mScarlet

    Plasmid
    #261323
    Purpose
    This plasmid can fuction as Moclo Level 1 (BsaI) or Level 2 (BpiI) acceptors and as starting backbones for developing vectors for other hosts through AarI-mediated Golden Gate assembly.
    Depositor
    Karen Sarkisyan
    Type
    Empty backbone
    Use
    Other
    Available since
    Sept. 18, 2026
    Availability
    Academic Institutions and Nonprofits only
    261323-plasmid-map-sequence-id-521397

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Showing: 261 - 280 of 5501 results