We narrowed to 866 results for: transfection

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C204

Plasmid

#174358

Purpose

For expressing siRNA-resistant human PABPC1 in human cell lines via transfection

Depositor

David Bartel

Article

Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

Insert

PABPC1 (PABPC1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Multiple silent mutations introduced to be resist…

Available Since

Feb. 19, 2022

Availability

Academic Institutions and Nonprofits only

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SEC13 in pRK5

Plasmid

#154215

Purpose

Untagged SEC13 cDNA expression vector for transfections

Depositor

David Sabatini

Article

Orozco et al Nat Metab. 2020 Sep;2(9):893-901. doi: 1

Insert

Sec13

Tags

NONE

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pARv-RFP

Plasmid

#60021

Purpose

pARv-RFP is a fluorescent reporter plasmid, that allows fast and easy evaluation of ZFN, TALEN or CRISPR/Cas9 activity in the transfected cells.

Depositor

Radislav Sedláček

Article

Kasparek et al FEBS Lett. 2014 Sep 18. pii: S0014-5793(

Type

Empty backbone

Expression

Mammalian

Available Since

Nov. 25, 2014

Availability

Academic Institutions and Nonprofits only

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sgRNA AAVS1

Plasmid

#128119

Purpose

Co-expresses sgRNA AAVS1, SpyCas9 scaffold (F+E) and GFP (transfection marker)

Depositor

Dominik Niopek

Article

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Insert

sgRNA AAVS1 + GFP

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

RSV/U6

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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sgRNA EMX1

Plasmid

#128121

Purpose

Co-expresses sgRNA EMX1, SpyCas9 scaffold (F+E) and GFP (transfection marker)

Depositor

Dominik Niopek

Article

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Insert

sgRNA EMX1 + GFP

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

RSV/U6

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCX:Gachapin-4A-P2A-mCherry

Plasmid

#251135

Purpose

Gachapin-A with a transfection marker mCherry

Depositor

Takeharu Nagai

Article

Kanadome et al Cell Rep Methods. 2026 Jan 23:101292. do

Insert

Gachapin-4A

Tags

P2A-mCherry

Expression

Mammalian

Promoter

Chicken β-actin promoter

Available Since

Feb. 18, 2026

Availability

Academic Institutions and Nonprofits only

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pCX:Gachapin-C2-P2A-mCherry

Plasmid

#251138

Purpose

Gachapin-C with a transfection marker mCherry

Depositor

Takeharu Nagai

Article

Kanadome et al Cell Rep Methods. 2026 Jan 23:101292. do

Insert

Gachapin-C2

Tags

P2A-mCherry

Expression

Mammalian

Promoter

Chicken β-actin promoter

Available Since

Feb. 18, 2026

Availability

Academic Institutions and Nonprofits only

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pCX:Gachapin-4B-P2A-EBFP2

Plasmid

#251136

Purpose

Gachapin-B with a transfection marker EBFP2

Depositor

Takeharu Nagai

Article

Kanadome et al Cell Rep Methods. 2026 Jan 23:101292. do

Insert

Gachapin-4B

Tags

P2A-EBFP2

Expression

Mammalian

Promoter

Chicken β-actin promoter

Available Since

Feb. 18, 2026

Availability

Academic Institutions and Nonprofits only

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pHS-MRscRNA

Plasmid

#240214

Purpose

Mammalian vector for transient expression of theophylline-responsive MR-scRNA with mCherry transfection marker

Depositor

Wilfred Chen

Article

Stohr et al Nucleic Acids Res. 2025 Nov 13;53(21):gk

Insert

Theophylline MR-scRNA targeting TET3G promoter

Expression

Mammalian

Promoter

U6

Available Since

Nov. 24, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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TVMV Protease-mCherry

Plasmid

#243794

Purpose

Encodes for mammalian expression of TVMV protease input. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

TVMV protease

Expression

Mammalian

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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Tol2-GgVCL/WT

Plasmid

#162787

Purpose

CONTROL_transfection of mammalian cells with WT Vcl under a β-actin promoter, with IRES-GFP allowing identification of transfected cells

Depositor

Laura Lafon-Hughes

Article

et al PeerJ

Insert

Gallus gallus vinculin (VCL Chicken)

Tags

IRES-GFP

Expression

Bacterial and Mammalian

Mutation

WT

Promoter

beta actin

Available Since

May 3, 2021

Availability

Academic Institutions and Nonprofits only

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sgRNA HBB

Plasmid

#128120

Purpose

Co-expresses sgRNA HBB, SpyCas9 scaffold (F+E) and GFP (transfection marker)

Depositor

Dominik Niopek

Article

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Insert

sgRNA HBB + GFP

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

RSV/U6

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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sgRNA RUNX

Plasmid

#128122

Purpose

Co-expresses sgRNA RUNX, SpyCas9 scaffold (F+E) and GFP (transfection marker)

Depositor

Dominik Niopek

Article

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Insert

sgRNA RUNX + GFP

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

RSV/U6

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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sgRNA HEK

Plasmid

#128123

Purpose

Co-expresses sgRNA HEK, SpyCas9 scaffold (F+E) and GFP (transfection marker)

Depositor

Dominik Niopek

Article

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Insert

sgRNA HEK + GFP

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

RSV/U6

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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Thy1.1-muSOX D219A

Plasmid

#131704

Purpose

encodes Thy1.1 followed by a P2A site and muSOX D219A catalytic mutant for enriching pure populations of transfected cells

Depositor

Britt Glaunsinger

Article

Gilbertson et al Elife. 2018 Oct 3;7. pii: 37663. doi: 10

Insert

Thy1.1-muSOX

Expression

Mammalian

Mutation

Aspartic acid 219 mutated to an alanine

Promoter

CMV

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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c-Fos

Plasmid

#187905

Purpose

Expresses c-Fos in transfected cells

Depositor

Jennifer Mitchell

Article

Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

Insert

cFos (FOS Human)

Expression

Mammalian

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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c-Jun

Plasmid

#187902

Purpose

Expresses c-Jun in transfected cells

Depositor

Jennifer Mitchell

Article

Mitchell et al Endocrinology. 2005 Apr;146(4):2048-54.

Insert

c-Jun (JUN Human)

Expression

Mammalian

Available Since

Oct. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pGL3-U6-sgRNA-PGK- mRFP-T2A-PuroR

Plasmid

#194925

Purpose

mRFP and T2A linker are inserted in between the hPGK promoter and the puromycin resistance gene (PuroR) on pGL3-U6-sgRNA-PGK-puromycin to allow simultaneous monitoring and enrichment of transfected ho

Depositor

Hao Xu

Article

Ayo et al J Genome Ed Regul. 2022;2:246146. doi: 1

Type

Empty backbone

Use

CRISPR

Promoter

hPGK

Available Since

May 30, 2023

Availability

Academic Institutions and Nonprofits only

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HRV-3C Protease-mCherry

Plasmid

#243792

Purpose

Encodes for mammalian expression of HRV-3C protease input. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

HRV-3C protease

Expression

Mammalian

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pCX:Gachapin-4A-P2A-mCherry-NLS

Plasmid

#251133

Purpose

Gachapin-A with a transfection marker mCherry-NLS

Depositor

Takeharu Nagai

Article

Kanadome et al Cell Rep Methods. 2026 Jan 23:101292. do

Insert

Gachapin-4A

Tags

P2A-mCherry-NLS

Expression

Mammalian

Promoter

Chicken β-actin promoter

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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