We narrowed to 5,995 results for: ATS

1. pAAV_GR-RE_MLPmin_BC1094-luc2

   Plasmid

   #227114

   Purpose

   Glucocorticoid receptor response element for luciferase and barcode assays; barcode BC1094

   Depositor

   Michael Wehr

   Article

   Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

   Insert

   12x clustered GR response element linked to MLP minimal promoter driving barcode 1094 and a luciferase reporter gene
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. FRB-GFP-Gab1(Y628F/Y660F)

   Plasmid

   #188658

   Purpose

   FRB coupled to GFP tagged Gab1 with Shp2 binding sites mutated to Phe

   Depositor

   Andrei Karginov

   Article

   Fauser et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

   Insert

   GRB associated binding protein 1 (Gab1 Mouse)
   

   Use

   Tags

   FRB and GFP

   Expression

   Mammalian

   Mutation

   Y628F and Y660F

   Promoter

   CMV

   Available since

   Aug. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. MOM603

   Plasmid

   #133242

   Purpose

   expresses full-length human KIF1A fused with mScarlet and StrepII tag in insect cells

   Depositor

   Shinsuke Niwa

   Article

   Chiba et al Proc Natl Acad Sci U S A. 2019 Aug 27. p

   Insert

   kif1a (KIF1A Human)
   

   Use

   Tags

   StrepII tag and mScarlet

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin promoter

   Available since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1 SARS-CoV-2 S D614

   Plasmid

   #158074

   Purpose

   Expresses SARS-CoV-2 spike protein

   Depositor

   Jeremy Luban

   Article

   Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

   Insert

   SARS-CoV-2 spike (S )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST-DHFR F[1,2]-N (TRP1)

   Plasmid

   #177797

   Purpose

   Gateway destination vector to insert genes of interest having a N-terminal DHFR F[1,2] fusion for DHFR-PCA/BFG-PCA assays.

   Depositor

   Christian Landry

   Article

   Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

   Type

   Empty backbone

   Use

   Tags

   DHFR F[1,2]

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pICE-HA-Ku70-siR-WT

   Plasmid

   #82329

   Purpose

   Plasmid for constitutive or doxycycline-inducible expression of wild-type human Ku70 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

   Depositor

   Sébastien Britton, Patrick Calsou

   Article

   Lamaa et al EMBO Rep. 2016 Apr;17(4):508-18. doi: 10

   Insert

   Ku70 (XRCC6 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-tet

   Available since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCEP4-HLA-c-myc-optiT1R3 ECD

   Plasmid

   #113960

   Purpose

   mammalian expression plasmid for c-myc-tagged human codon-optimized human T1R3 extracellular domain with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

   Depositor

   Erik Procko

   Article

   Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

   Insert

   T1R3 (TAS1R3 Human)
   

   Use

   Tags

   Signal/leader sequence from HLA class I histocomp…

   Expression

   Mammalian

   Mutation

   residues 21-563

   Promoter

   cmv

   Available since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. FRB-GFP-Gab2(Y604F/Y634F)

   Plasmid

   #188659

   Purpose

   FRB coupled to GFP tagged Gab2 with Shp2 binding sites mutated to Phe

   Depositor

   Andrei Karginov

   Article

   Fauser et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

   Insert

   GRB associated binding protein 2 (Gab2 Mouse)
   

   Use

   Tags

   FRB and GFP

   Expression

   Mammalian

   Mutation

   Y604F and Y634F

   Promoter

   CMV

   Available since

   Aug. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFUGW ORANGE Tubb3-GFP KI _ mCherry-KASH

   Plasmid

   #131508

   Purpose

   Endogenous tagging of β3 Tubulin: C-terminal (amino acid position: STOP codon)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Tubb3, Tubb3 Rat, Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hUBC

   Available since

   Sept. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFUW-TetO-Fos

    Plasmid

    #131593

    Purpose

    doxycycline-inducible expression of mouse c-Fos in mammalian cells

    Depositor

    Kateri Moore, Filipe Pereira

    Article

    Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

    Insert

    FBJ osteosarcoma oncogene (Fos Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-mCMV

    Available since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFUW-tetO-Tcf4

    Plasmid

    #139806

    Purpose

    doxycycline-inducible expression of mouse Tcf4 in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

    Insert

    Tcf4 (Tcf4 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-mCMV

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDF-Az

    Plasmid

    #197101

    Purpose

    To express the azidophenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into azidophenylalanine

    Depositor

    Kensaku Sakamoto

    Article

    Yamaguchi et al Bioconjug Chem. 2016 Jan 20;27(1):198-20

    Insert

    TyrRS variant, amber suppressor tRNA, kanamycin resistance gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Thr32, Asn107, Pro158, Leu159, Gln162, Arg286 (Ty…

    Promoter

    Available since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pIgK-2.1-FLAG

    Plasmid

    #225829

    Purpose

    Mouse IgK chain specific for chicken gamma globulin (clone 2.1). Used to produce FLAG-tagged monoclonal antibodies for use as tag-specific ELISA standards.

    Depositor

    Gabriel Victora

    Article

    Schiepers et al Nature. 2023 Mar;615(7952):482-489. doi:

    Insert

    Mouse anti-CGG IgK chain, Flag-tagged
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIgK-2.1-StreptagII

    Plasmid

    #225830

    Purpose

    Mouse IgK chain specific for chicken gamma globulin (clone 2.1). Used to produce StreptagII-tagged monoclonal antibodies for use as tag-specific ELISA standards.

    Depositor

    Gabriel Victora

    Article

    Schiepers et al Nature. 2023 Mar;615(7952):482-489. doi:

    Insert

    Mouse anti-CGG IgK chain, Streptag-II-tagged
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-syn-F14F15S-splitTVA-EGFP-tTAv2

    Plasmid

    #183352

    Purpose

    Flp-dependent helper virus for monosynaptic tracing; to be coinjected with pAAV-TREtight-mTagBFP2-B19G (Addgene #100799)

    Depositor

    Ian Wickersham

    Article

    Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

    Insert

    TVA, EGFP and tTA
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pORANGE GFP-Clta KI

    Plasmid

    #131483

    Purpose

    Endogenous tagging of Clathrin light chain α: N-terminal (amino acid position: M1)

    Depositor

    Harold MacGillavry

    Article

    Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

    Insert

    gRNA and GFP donor (Clta Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and Cbh

    Available since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. RFP-(SUMO)6-(SIM)10

    Plasmid

    #122027

    Purpose

    Mammalian expression plasmid for multivalent scaffold of (SUMO)6-(SIM)10 biomolecular condensates to recruit SUMO-containing client proteins.

    Depositor

    Michael Rosen

    Article

    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

    Insert

    6 repeats of human SUMO3 and 10 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (PIAS2, SUMO3 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGGS-hPhyBY276H-mCherry-HRasCT

    Plasmid

    #100540

    Purpose

    Expresses Phytochrome B-Y276H (1-908 aa) fused with mCherry and Hras C-terminus in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

    Insert

    Phytochrome B (1-908 aa, Y276H mutation) (PHYB Mustard Weed)
    

    Use

    Tags

    mCherry fused with HRas C-terminus for plasma mem…

    Expression

    Mammalian

    Mutation

    Human codon optimized PhyB (1-908 aa), Y276H muta…

    Promoter

    CAG promoter

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFUW-tetO-Bcl11b

    Plasmid

    #139831

    Purpose

    doxycycline-inducible expression of mouse Bcl11b in mammalian cells

    Depositor

    Filipe Pereira

    Article

    Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

    Insert

    Bcl11b (Bcl11b Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-mCMV

    Available since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pNK3203

    Plasmid

    #219749

    Purpose

    DVK_AF CIDAR vector encoding hispidin-synthase from Mycena citricolor under control of CMV promoter, for mammalian expression

    Depositor

    Alexander Mishin

    Article

    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi

    Insert

    pCMV - mcitHispS - tSV40
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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