We narrowed to 12,753 results for: CAR

1. pAW8ENdeI-c3HA-8XDSR

   Plasmid

   #49871

   Purpose

   for Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences together with 8 copies of the core DSR motif into the S. pombe urg1 locus

   Depositor

   Tony Carr

   Article

   Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   3XHA

   Expression

   Mutation

   Promoter

   Available since

   Dec. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAW8ENdeI-c3HA-4XDSR

   Plasmid

   #49869

   Purpose

   for Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences together with 4 copies of the core DSR motif into the S. pombe urg1 locus

   Depositor

   Tony Carr

   Article

   Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   3XHA

   Expression

   Mutation

   Promoter

   Available since

   Dec. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. BS.YxiN.Twin1.a207-368.wt

   Plasmid

   #13531

   Depositor

   David McKay

   Article

   Caruthers et al Acta Crystallograph Sect F Struct Biol C

   Insert

   B. subtilis DEAD-box helicase YxiN second domain
   

   Use

   Tags

   intein-chitin binding domain

   Expression

   Bacterial

   Mutation

   deleted amino acid residues 1-206 and 369-479

   Promoter

   Available since

   Jan. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJMP8835

   Plasmid

   #220902

   Purpose

   MobileCRiSPRi test system encoded on a kanR Tn7 transposon; mScarlet-I, PD-sgRNA (mscar targeting), and PG-dCas9-novo

   Depositor

   Jason Peters

   Article

   Hall et al Appl Environ Microbiol. 2024 Sep 26:e003

   Insert

   dCas9-novo
   

   Use

   CRISPR

   Tags

   3xMyc

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   Available since

   Sept. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pY2F

   Plasmid

   #53285

   Purpose

   Contains an Arabidopsis thaliana lcyE cDNA and produces an active lycopene epsilon-cyclase enzyme in E. coli. Use with pAC-BETA-At or pAC-BETAipi-At to produce alpha-carotene in E. coli.

   Depositor

   Francis X Cunningham Jr

   Article

   Cunningham FX et al Photosynth Res. 2007 May;92(2):245-59. E

   Insert

   lcyE (LUT2 Mustard Weed)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   April 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1 Neo(+) hSUMF1

   Plasmid

   #24386

   Depositor

   Carolyn Bertozzi

   Article

   Wu et al Proc Natl Acad Sci U S A. 2009 Mar 3. 10

   Insert

   hSUMF1 (SUMF1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 2, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. Hsp90 HA

   Plasmid

   #22487

   Depositor

   William Sessa

   Article

   Garcia-Cardena et al Nature. 1998 Apr 23. 392(6678):821-4.

   Insert

   Hsp90 HA (HSP90AB1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 20, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-ATR WT

   Plasmid

   #31611

   Depositor

   Aziz Sancar

   Article

   Jiang et al Mol Cell Biol. 2006 Jan . 26(1):39-49.

   Insert

   ATR (ATR Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. p415-GalL-Cas9-CYC1t

   Plasmid

   #43804

   Purpose

   A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the GalL promoter

   Depositor

   George Church

   Article

   Dicarlo et al Nucleic Acids Res

   Insert

   Human Optimized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GalL

   Available since

   March 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. p414-TEF1p-Cas9-CYC1t

    Plasmid

    #43802

    Purpose

    A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the TEF1 promoter

    Depositor

    George Church

    Article

    Dicarlo et al Nucleic Acids Res

    Insert

    Human Optimized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1 promoter

    Available since

    March 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. NfPolyA_pET21b

    Plasmid

    #215413

    Purpose

    Expression construct for NfPolyA gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    NfPolyA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMaCTag-Z12

    Plasmid

    #120055

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with mScarlet

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mScarlet
    

    Use

    Pcr template

    Tags

    mScarlet

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHCA/GAL4(1-93).ER.VP16

    Plasmid

    #108216

    Purpose

    Yeast expression vector for fusion of Gal4 DNA binding domain to estrogen receptor alpha hormone binding domain to VP16 transcriptional activation domain

    Depositor

    Didier Picard

    Article

    Louvion et al Gene 1993 Sep 6;131(1):129-34.

    Insert

    Gal4 DBD-ERalpha HBD-VP16
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    ERalpha HBD has G400V mutation

    Promoter

    ADH1

    Available since

    April 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET15bpATnp

    Plasmid

    #121137

    Purpose

    This vector encode a His6-tagged proteinA-Tnp(hyperactive form) fusion protein.

    Depositor

    Keji Zhao

    Article

    Carter et al Nat Commun. 2019 Aug 20;10(1):3747. doi:

    Insert

    Protein A-Tnp
    

    Use

    Tags

    his

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBMPC3

    Plasmid

    #31367

    Depositor

    Aziz Sancar

    Article

    Ye et al J Biol Chem. 2011 Jul 22;286(29):25891-9

    Insert

    Bmal1 (Bmal1 Mouse)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. myc-GABAAR Gamma2

    Plasmid

    #127619

    Purpose

    N-terminal myc-tagged Gamma2 subunit of GABAAR

    Depositor

    Josef Kittler

    Article

    Arancibia-Carcamo et al Proc Natl Acad Sci U S A. 2009 Oct 13;10

    Insert

    GABA-A receptor subunit gamma-2
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMC1SG5

    Plasmid

    #31282

    Depositor

    Aziz Sancar

    Article

    Ye et al J Biol Chem. 2011 Jul 22;286(29):25891-9

    Insert

    Cry1 (Cry1 Mouse)
    

    Use

    Tags

    Flag, His, and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pK-myc-Par3b

    Plasmid

    #19388

    Depositor

    Ian Macara

    Article

    Joberty et al Nat Cell Biol 2000 Aug;2(8):531-9.

    Insert

    Partitioning-defective protein 3b (PARD3 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Full length, isoform b.

    Promoter

    Available since

    March 31, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNMT

    Plasmid

    #42578

    Depositor

    James Hurley

    Article

    Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

    Insert

    N-myristoyl tranferase (NMT1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    two silent mutations T690C and T942C

    Promoter

    T7

    Available since

    Aug. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pSLHDR03_mCherry_tCD19_unique guide

    Plasmid

    #124062

    Purpose

    Construction of 1st donor for scarless genome editing with unique guide

    Depositor

    Matthew Porteus

    Article

    Ikeda et al Nat Methods. 2018 Dec;15(12):1045-1047.

    Inserts

    mCherry
    tCD19
    

    Use

    Mouse Targeting; Human targeting

    Tags

    Expression

    Mutation

    Promoter

    Human UbC modified

    Available since

    April 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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