We narrowed to 3,073 results for: N-EGFP

1. pEMS1387

   Plasmid

   #29186

   Purpose

   Expression of the eGFP reporter gene was not detected in the brain and eye.

   Depositor

   Elizabeth Simpson

   Article

   Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

   Insert

   Ple118 (HTR1A Human)
   

   Use

   Pleiades promoter project [sic, pleaides plieades]

   Tags

   EGFP-NLS

   Expression

   Mutation

   Promoter

   Available since

   Nov. 15, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEMS1216

   Plasmid

   #29110

   Purpose

   Expression of the eGFP reporter gene was not detected in the brain and eye.

   Depositor

   Elizabeth Simpson

   Article

   Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1

   Insert

   Ple98 (GPX3 Human)
   

   Use

   Pleiades promoter project [sic, pleaides plieades]

   Tags

   EGFP-NLS

   Expression

   Mutation

   Promoter

   Available since

   Oct. 3, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. V2R untagged

   Plasmid

   #208911

   Purpose

   encodes the human vasopressin receptor 2, without any tags

   Depositor

   Christian Gruber

   Article

   Dekan et al Chem Sci. 2021 Feb 4;12(11):4057-4062. d

   Insert

   AVPR2 (AVPR2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. V1bR untagged

   Plasmid

   #208925

   Purpose

   encodes the human vasopressin receptor 1b, without any tags

   Depositor

   Christian Gruber

   Article

   Dekan et al Chem Sci. 2021 Feb 4;12(11):4057-4062. d

   Insert

   AVPR1B (AVPR1B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. anxA2-GFP

   Plasmid

   #107196

   Purpose

   Expresses human annexin A2-GFP fusion protein for live cell imaging

   Depositor

   Volker Gerke, Ursula Rescher

   Article

   Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8

   Insert

   annexin A2 (ANXA2 Human)
   

   Use

   Tags

   Green Fluorescent Protein

   Expression

   Mammalian

   Mutation

   Ala residue 65 in human sequence replaced by Glu …

   Promoter

   CMV

   Available since

   June 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. alpha actinin delta ABD-GFP

   Plasmid

   #66935

   Purpose

   Mammalian expression of alpha actinin with actin binding domain (amino acids 30–253) deleted

   Depositor

   Michael Sheetz, Pere Roca-Cusachs

   Article

   Roca-Cusachs et al Proc Natl Acad Sci U S A. 2013 Apr 9;110

   Insert

   Alpha-actinin 1 (ACTN1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   actin binding domain (amino acids 30–253) is dele…

   Promoter

   CMV

   Available since

   June 30, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pBactin-Akna-mOrange2

   Plasmid

   #196865

   Purpose

   Expression of the centrosomal protein Akna fused to mOrange2

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   Akna-mOrange2 (Akna Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken bactin (plus Chicken bactin intron)

   Available since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmGFP-Sec16S

   Plasmid

   #15775

   Depositor

   Benjamin Glick

   Article

   Bhattacharyya et al Mol Biol Cell. 2007 Mar;18(3):839-49. do

   Insert

   Sec16S (SEC16B Human)
   

   Use

   Tags

   mEGFP

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Available since

   Nov. 21, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-Syn-FLEX-rc [Archon1-KGC-GFP-ER2]

   Plasmid

   #115893

   Purpose

   AAV-mediated expression of Archon1-KGC-GFP-ER2 under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.

   Depositor

   Edward Boyden

   Article

   Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

   Has Service

   AAV8

   Insert

   Archon1-KGC-EGFP-ER2
   

   Use

   AAV

   Tags

   ER2, GFP, and KGC

   Expression

   Mammalian

   Mutation

   Promoter

   Syn

   Available since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYO395

    Plasmid

    #235734

    Purpose

    Expression of human VPS34 complex I in mammalian cells

    Depositor

    Roger Williams

    Article

    Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

    Inserts

    ATG14 (ATG14 Human)
    EGFP
    BECN1 (BECN1 Human)
    PIK3C3 (PIK3C3 Human)
    PIK3R4 (PIK3R4 Human)
    

    Use

    Tags

    ZZ-3xTEV

    Expression

    Mammalian

    Mutation

    N256S

    Promoter

    CMV

    Available since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCBh-T7-SpCas9(KRTMQR)-P2A-mTagBFP2(RAS3591)

    Plasmid

    #223106

    Purpose

    pCBh Human expression plasmid for SpCas9 enzyme with KRTMQR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(KRTMQR)-P2A-mTagBFP2
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-mTagBFP2

    Expression

    Mammalian

    Mutation

    SpCas9(KRTMQR)=D1135K, S1136R, G1218T, E1219M, R1…

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCBh-T7-SpCas9(KRHWMR)-P2A-mTagBFP2(RAS3594)

    Plasmid

    #223107

    Purpose

    pCBh Human expression plasmid for SpCas9 enzyme with KRHWMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(KRHWMR)-P2A-mTagBFP2
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-mTagBFP2

    Expression

    Mammalian

    Mutation

    SpCas9(KRHWMR)=D1135K, S1136R, G1218H, E1219W, R1…

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCBh-T7-SpCas9(MRRWYR)-P2A-mTagBFP2(RAS3598)

    Plasmid

    #223108

    Purpose

    pCBh Human expression plasmid for SpCas9 enzyme with MRRWYR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(MRRWYR)-P2A-mTagBFP2
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-mTagBFP2

    Expression

    Mammalian

    Mutation

    SpCas9(MRRWYR)=D1135M, S1136R, G1218R, E1219W, R1…

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCBh-T7-SpCas9(MRRWMR)-P2A-mTagBFP2(RAS3579)

    Plasmid

    #223109

    Purpose

    pCBh Human expression plasmid for SpCas9 enzyme with MRRWMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-mTagBFP2

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9(MRRWMR)-P2A-mTagBFP2
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-mTagBFP2

    Expression

    Mammalian

    Mutation

    SpCas9(MRRWMR)=D1135M, S1136R, G1218R, E1219W, R1…

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Thy1.1-G5

    Plasmid

    #234839

    Purpose

    pMP71 backbone. Expresses Thy1.1 with N-terminal 5xGly (G5)

    Depositor

    Gabriel Victora

    Article

    Nakandakari-Higa et al Nature. 2024 Mar;627(8003):399-406. doi:

    Insert

    Thy1.1 (Thy1 Mouse)
    

    Use

    Retroviral

    Tags

    EGFP-P2A-5xGly

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR-UCOE-EF1a-Zim3-dCas9-P2A-GFP

    Plasmid

    #188778

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Jonathan Weissman, Marco Jost

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS and Zim3 KRAB-NLS fusion

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CARPID BASU-dCasRx

    Plasmid

    #153209

    Purpose

    express CARPID BASU-dCasRx fusion protein in mammalian cells

    Depositor

    Liang Zhang, Jian Yan

    Article

    Yi et al Nat Methods. 2020 Jun 22. pii: 10.1038/s

    Insert

    BASU-dCasRx
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    R239A/H244A/R858A/H863A in RfxCas13d

    Promoter

    EF-1a

    Available since

    June 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPC1000

    Plasmid

    #174829

    Purpose

    Lentiviral Repair-seq vector containing CRISPRi sgRNA and SaPE2 prime edit site

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Inserts

    CRISPRi SpCas9 sgRNA cassette
    PuroR-P2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Detailed in manuscript

    Promoter

    EF1a and mouse U6

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. TV-hPITX3-forward

    Plasmid

    #22073

    Depositor

    Rudolf Jaenisch

    Article

    Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

    Insert

    PITX3 (ASMD Human)
    

    Use

    Cre/Lox; Zfn donor

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    Available since

    Oct. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. macroH2A2-GFP (pc2191)

    Plasmid

    #223709

    Purpose

    Expression of macroH2A2 tagged N-terminal to GFP

    Depositor

    Cristina Cardoso

    Article

    Arroyo et al Nucleic Acids Res. 2024 Aug 27:gkae734.

    Insert

    macroH2A2-GFP (MACROH2A2, H2AFY2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge