We narrowed to 5,032 results for: PID

1. pAAV-hSyn-JEDI-1P-WPRE

   Plasmid

   #202613

   Purpose

   Genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector for neuron-specific expression using the promoter hSyn

   Depositor

   Francois St-Pierre

   Article

   Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

   Insert

   JEDI-1P
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available since

   June 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTS110 pHAGE2 CMVtetO2 MCS-22xGS-Alfa-P2A-TagBFP

   Plasmid

   #199363

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally ALFA-P2A-BFP-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   ALFA-P2A-TagBFP

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-EGFP-Rac1-wt

   Plasmid

   #12980

   Depositor

   Gary Bokoch

   Article

   Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

   Insert

   Rac1 (RAC1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTS97 pHAGE2 CMVtetO2 GFP-22xGS-SUMOstar-MCS

   Plasmid

   #199350

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-SUMOstar-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EGFP-SUMOstar

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMSP1E3

   Plasmid

   #20064

   Depositor

   Stephen Sligar

   Article

   Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

   Insert

   MSP1E3 (APOA1 synthetic gene)
   

   Use

   Tags

   6-His

   Expression

   Bacterial

   Mutation

   "extended" MSP1; contains repeats of he…

   Promoter

   Available since

   March 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIRES-Ap2a2-FKBP

   Plasmid

   #46944

   Purpose

   Co-expresses siRNA-resistant, FKBP-tagged AP-2 alpha subunit and G418 resistance gene in mammalian cells

   Depositor

   Margaret Robinson

   Article

   Robinson et al Dev Cell. 2010 Feb 16;18(2):324-31. doi:

   Insert

   Ap2a2-FKBP
   

   Use

   Tags

   Mainly mouse Ap2a2 cDNA, with a fragment of brain…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CMV-CD4-sTC-M13-GFP-P2A-CaM-V5-sTN

   Plasmid

   #219781

   Purpose

   CaST-P2A for HEK cell expression

   Depositor

   Christina Kim

   Article

   Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. d

   Insert

   CD4-sTC-M13-GFP-P2A-CaM-V5-sTN
   

   Use

   AAV

   Tags

   HA and V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pETDuet RagA-RagC

   Plasmid

   #99641

   Purpose

   Bacterial expression of wildtype Rag GTPase dimer

   Depositor

   Kuang Shen, David Sabatini

   Article

   Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

   Inserts

   RagA (RRAGA Human)
   RagC (RRAGC Human)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAGGS-Hoxc6

   Plasmid

   #45553

   Depositor

   Jeremy Dasen

   Article

   Lacombe et al PLoS Genet. 2013 Jan;9(1):e1003184. doi:

   Insert

   Hoxc6 (Hoxc6 Mouse)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   CMV / b-actin

   Available since

   Aug. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pRV.GFP FOXP3

    Plasmid

    #13250

    Depositor

    Anjana Rao

    Article

    Wu et al Cell. 2006 Jul 28. 126(2):375-87.

    Insert

    FOXP3 (FOXP3 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET21-pelB-LaG-16

    Plasmid

    #172746

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-16; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-16
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSCIP-mB2M

    Plasmid

    #154091

    Purpose

    Self-Cutting and Integrating CRISPR backbone targeting murine B2M

    Depositor

    Scott McComb

    Article

    Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

    Insert

    sgRNA and BAIT targeting murine B2M locus
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. Luc-noPS

    Plasmid

    #177940

    Purpose

    Transient mammalian expression of Luc2 (firefly luciferase). Lacks packaging sequence and is used as a negative control transfer plasmid for SARS-CoV-2 virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    Luc2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMSP1E2

    Plasmid

    #20063

    Depositor

    Stephen Sligar

    Article

    Denisov et al J Am Chem Soc. 2004 Mar 24. 126(11):3477

    Insert

    MSP1E2 (APOA1 synthetic gene)
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    "extended" MSP1; contains repeats of he…

    Promoter

    Available since

    March 16, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMuLE ENTR tdTomato L5-L2

    Plasmid

    #62185

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing tdTomato. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Insert

    tdTomato
    

    Use

    Mule gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRK5-PLBD2-6xHis

    Plasmid

    #213606

    Purpose

    Expresses PLBD2-6xHis in mammalian cells

    Depositor

    Monther Abu-Remaileh

    Article

    Nyame et al Mol Cell. 2024 Apr 4;84(7):1354-1364.e9.

    Insert

    phospholipase B domain containing 2 (PLBD2 Human)
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET21-pelB-LaG-16-4GS-LaG-2

    Plasmid

    #172764

    Purpose

    Bacterial expression of dimerized anti-GFP nanobodies LaG-16 and LaG-2 (4GS linker)

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-16-4GS-LaG-2
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMK288 (mAID-Bsr)

    Plasmid

    #72826

    Purpose

    C-terminal tagging with mAID using CRISPR/Cas9

    Depositor

    Masato Kanemaki

    Article

    Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

    Insert

    mAID-Bsr
    

    Use

    CRISPR

    Tags

    mAID

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-PM-ExRai-AktAR2

    Plasmid

    #184050

    Purpose

    Plasma membrane-targeted ExRai-AktAR2

    Depositor

    Jin Zhang

    Article

    Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

    Insert

    PM-ExRai-AktAR2
    

    Use

    Tags

    N-terminal targeting sequence from Lyn kinase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET17b-yebST

    Plasmid

    #175804

    Purpose

    Complementation of yebST knockout in E. coli K-12

    Depositor

    Ian Henderson, Gira Bhabha, Damian Ekiert

    Article

    Isom et al Sci Rep. 2017 Aug 17;7(1):8608. doi: 10.

    Insert

    yebST
    

    Use

    Tags

    None

    Expression

    Bacterial

    Mutation

    silent mutations to introduce restriction sites

    Promoter

    T7

    Available since

    April 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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