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pDEST-APIC-Flp1

Plasmid

#112806

Purpose

gateway cloning destination vector to express enhancer driven Flip1 in Drosophila

Depositor

Chun Han

Article

Poe et al Proc Natl Acad Sci U S A. 2017 Sep 19;11

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pAH35 (Pmyo-2::cGAL-N::let-858 3'UTR)

Plasmid

#107134

Purpose

cGAL-N driver under the control of C. elegans myo-2 promoter

Depositor

Paul Sternberg

Article

Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

Insert

NLS::cGAL(DBD)::gp41-1-N-intein

Expression

Worm

Promoter

C. elegans myo-2 promoter

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pGP4G-TPLRD1-ccdB

Plasmid

#83877

Purpose

Destination vector for integration at yeast Trp locus with N-terminal TPL repression domain (RD) for auxin response assays

Depositor

Jennifer Nemhauser

Article

Pierre-Jerome et al Proc Natl Acad Sci U S A. 2014 Jul 1;111

Type

Empty backbone

Use

Gateway Cloning

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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GST-CHMP4C (216-233) (pGEX2T(TEV))

Plasmid

#80635

Purpose

Residues 216-233 of CHMP4C; Bacterial Expression Vector; TEV-cleavable GST tag; Sundquist Lab Internal ID: WISP06-202

Depositor

Wesley Sundquist

Article

McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

Insert

CHMP4C (CHMP4C Human)

Tags

GST

Expression

Bacterial

Mutation

Residues 216-233

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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GST-CHMP4A (1-204) (pGEX2T(TEV))

Plasmid

#80632

Purpose

Residues 1-204 of CHMP4A; Bacterial Expression vector; TEV-cleavable GST tag; Sundquist Lab internal ID: WISP06-198

Depositor

Wesley Sundquist

Article

McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

Insert

CHMP4A (CHMP4A Human)

Tags

GST

Expression

Bacterial

Mutation

Residues 1-204

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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GST-CHMP4B (205-224) (pGEX2T(TEV))

Plasmid

#80634

Purpose

Residues 205-224 of CHMP4B, Bacterial Expression Vector; TEV-cleavabel GST tag; Sundquist Lab Internal ID: WISP06-201

Depositor

Wesley Sundquist

Article

McCullough et al Proc Natl Acad Sci U S A. 2008 Jun 3;105

Insert

CHMP4B (CHMP4B Human)

Tags

GST

Expression

Bacterial

Mutation

Residues 205-224

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pLL3.7 shRNA against CRTC3

Plasmid

#72712

Purpose

Lentiviral expression of shRNA against CRTC3 (3rd generation)

Depositor

Freddy Jeanneteau

Article

Jeanneteau et al Proc Natl Acad Sci U S A. 2012 Jan 24;10

Insert

CRTC3

Use

Lentiviral

Tags

CMV-EGFP

Expression

Mammalian

Promoter

U6

Available Since

Feb. 9, 2016

Availability

Academic Institutions and Nonprofits only
pCNL-C1_Rab11a

Plasmid

#65706

Purpose

Expresses CNL-Rab11a in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Rab11a (Rab11a Mouse)

Use

Luciferase

Tags

CNL

Expression

Mammalian

Promoter

CMV

Available Since

June 23, 2015

Availability

Academic Institutions and Nonprofits only

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pYNL-N1_TUBB5

Plasmid

#65678

Purpose

Expresses beta-tubulin-YNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

beta-tubulin class V

Use

Luciferase

Tags

YNL

Expression

Mammalian

Promoter

CMV

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only
pYNL-N1_zyxin

Plasmid

#65681

Purpose

Expresses Zyxin-YNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

zyxin (Zyx Mouse)

Use

Luciferase

Tags

YNL

Expression

Mammalian

Promoter

CMV

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only
pCNL-N1_zyxin

Plasmid

#65682

Purpose

Expresses Zyxin-CNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

zyxin (Zyx Mouse)

Use

Luciferase

Tags

CNL

Expression

Mammalian

Promoter

CMV

Available Since

June 17, 2015

Availability

Academic Institutions and Nonprofits only
pCNL-C1_H2B

Plasmid

#65703

Purpose

Expresses CNL-H2B in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

histone H2B

Use

Luciferase

Tags

CNL

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2015

Availability

Academic Institutions and Nonprofits only

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pCNL-N1_Mito

Plasmid

#65670

Purpose

Expresses Mito-CNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

cytochrome c oxidase subunit VIII

Use

Luciferase

Tags

CNL

Expression

Mammalian

Promoter

CMV

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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pYNL-N1_3xNLS

Plasmid

#65672

Purpose

Expresses NLS-YNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

three copies of SV40 nuclear localization signal

Use

Luciferase

Expression

Mammalian

Promoter

CMV

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pCNL-N1_3xNLS

Plasmid

#65673

Purpose

Expresses NLS-CNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

three copies of SV40 nuclear localization signal

Use

Luciferase

Tags

CNL

Expression

Mammalian

Promoter

CMV

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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pDY4

Plasmid

#53589

Purpose

Produces an active Lactuca sativa (lettuce) lycopene epsilon-cyclase in E. coli. Use with pAC-LYC or pAC-LYCipi to produce epsilon-carotene in E. coli.

Depositor

Francis X Cunningham Jr

Article

Cunningham FX et al Proc Natl Acad Sci U S A. 2001 Feb 27;98

Insert

lcyE

Expression

Bacterial

Promoter

lac

Available Since

Jan. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pBT314_pR26-U-attP-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5

Plasmid

#52547

Purpose

Targeting vector for homologous recombination in mouse ES cells; Rosa26 locus

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attP (full length)
Neo
PhiC31o integrase

Expression

Mammalian

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pBT366_pattB-Hb9-GFP-pA-FRT5

Plasmid

#52552

Purpose

for PhiC31 integrase-mediated integration of pHb9-GFP

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attB (full length)
GFP

Expression

Mammalian

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pBT307_pBS-U-attPx3-FRT5-pSV40-Neo-pA-PhiC31o-pA-FRT5

Plasmid

#52540

Purpose

Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attPx3 (70 bp each)
Neo
PhiC31o integrase

Expression

Mammalian

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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pT7ddx19gRNA

Plasmid

#47932

Purpose

in vitro transcription of ddx19 gRNA

Depositor

Wenbiao Chen, Susan Wente

Article

Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

Insert

ddx19 gRNA (ddx19 Zebrafish)

Use

CRISPR

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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