We narrowed to 6,501 results for: nls
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Plasmid#89994PurposeDonor template for generation of NGN3-ntdTomato reporter cell linesDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNGN3-T2A-NLS-TdTomato-ires-puro-BGHpA-PGK-puro (NEUROG3 Human)
UseCRISPRPromoternoneAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-BPNLS-ABE8e-iSpyMac-BPNLS-P2A-EGFP (LLH528)
Plasmid#208289PurposeCMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with iSpyMac(D10A) and P2A-EGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertpCMV-T7-BPNLS-ABE8e-iSpyMac-BPNLS-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS and BPNLS-P2A-EGFPExpressionMammalianMutationABE8e mutations in TadA; mutations in iSpyMacPromoterCMV and T7Available sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
CARMEN
Plasmid#240464PurposeCompartment-specific measurements of Ca²⁺ dynamics by intensiometric Ca²⁺ biosensors targeted to mitochondria, cytosol and ERDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCARMEN
ExpressionMammalianAvailable sinceOct. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBDKRB2_TCS(Q’G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1
Plasmid#101107PurposeEncodes dCas9(C)-VP64 with SAM components fused to BDKRB2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBDKRB2-dCas9(N)
UseCRISPR and Synthetic BiologyTagsFlag and HAExpressionMammalianAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMZ-177
Plasmid#195425PurposeTransient, constitutive expression of synZiFTR: ZF2-p65DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertpUb - 3XFLAG - NLS - ZF2-6 (ZF2) - p65 - RbGpA
ExpressionMammalianAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMZ-179
Plasmid#195427PurposeTransient, constitutive expression of synZiFTR: ZF4-p65DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertpUb - 3XFLAG - NLS - ZF4-8 (ZF4) - p65 - RbGpA
ExpressionMammalianAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMZ-182
Plasmid#195430PurposeTransient, constitutive expression of synZiFTR: ZF7-p65DepositorInsertpUb - 3XFLAG - NLS - ZF7-3 (ZF7) - p65 - RbGpA
ExpressionMammalianAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMZ-183
Plasmid#195431PurposeTransient, constitutive expression of synZiFTR: ZF8-p65DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertpUb - 3XFLAG - NLS - ZF8-1 (ZF8) - p65 - RbGpA
ExpressionMammalianAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMZ-184
Plasmid#195432PurposeTransient, constitutive expression of synZiFTR: ZF9-p65DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertpUb - 3XFLAG - NLS - ZF9-2 (ZF9) - p65 - RbGpA
ExpressionMammalianAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMZ-186
Plasmid#195434PurposeTransient, constitutive expression of synZiFTR: ZF11-p65DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertpUb - 3XFLAG - NLS - ZF11-1 (ZF11) - p65 - RbGpA
ExpressionMammalianAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a(+)v2_TwinStrep-SUMO-φC31(PhiC31)-8xHis
Plasmid#246982PurposeExpresses E. coli codon-optimized TwinStrep-SUMO-tagged φC31(PhiC31) integrase with N- and C-terminal NLS, using an improved pET design from Shilling et al. 2020.DepositorInsertφC31(PhiC31)
UseSynthetic BiologyTags8xHis, Nucleoplasmin, SUMO, SV40, and TwinStrepExpressionBacterialPromoterT7Available sinceOct. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2560 - pEXP(mosTI, unc-119)[Peft-3 | ceGFP(PATC, NLS) | tbb-2 3'UTR]
Plasmid#159825PurposeSingle copy or array insertion by MosTI using split unc-119 selectionDepositorInsertpEXP(mosTI, unc-119)[Peft-3 | ceGFP(PATC, NLS) | tbb-2 3'UTR]
ExpressionWormMutationNot applicableAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
CL7_2xNLS_iGeoCas9(C2)_2xNLS_His
Plasmid#222988PurposeBacterial expression of iGeoCas9(C2) constructDepositorInsertCL7_2xNLS_iGeoCas9(C2)_2xNLS_His
UseCRISPRTagsCL7 and HisExpressionBacterialPromotertacAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
CL7_2xNLS_iGeoCas9(C1)_2xNLS_His
Plasmid#222987PurposeBacterial expression of iGeoCas9(C1) constructDepositorInsertCL7_2xNLS_iGeoCas9(C1)_2xNLS_His
UseCRISPRTagsCL7 and HisExpressionBacterialPromotertacAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
U6_sgRNA_CAG_ABE7.10_St1Cas9_LMD9
Plasmid#136660PurposeExpresses ABE St1Cas9 LMD9 in mammalian cells along with its U6-driven sgRNADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsSt1Cas9 LMD-9
sgRNA for St1Cas9
ABE7.10
UseCRISPR and Synthetic BiologyTagsSV40 NLS and hSt1Cas9ExpressionMammalianPromoterCAG and U6Available sinceFeb. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hAID-BE3
Plasmid#113417PurposeExpresses hAID-BE3 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthAID-BE3 (AICDA Human, S. pyogenes and Bacteriophage PBS2)
UseCRISPRExpressionMammalianPromoterCMVAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLJM5-C234S PHGDH
Plasmid#83902Purposestable overexpressionDepositorInsertPhosphoglycerate dehydrogenase (PHGDH Human)
UseLentiviralExpressionMammalianMutationquikchanged cysteine 234 to serinePromoterCMVAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
TV_ANXA1-p2a-mScarlet-nls-p2a-icasp9_pgk-blast
Plasmid#251171PurposeTargeting vector for C-terminal knockin at ANXA1 locus to enable reporting of expression by mScarlet and depletion of positive cells with iCasp9.DepositorInsertp2a-mScarlet-nls-icasp9_pgk-blast flanked by homology arms for C-terminal targeting of human ANXA1 (ANXA1 Human)
UseCRISPRTagsp2a-mScarlet-nls-p2a-icasp9Available sinceFeb. 20, 2026AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2309 - [1-2] ENTR - Fluor - mCardinal(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
Plasmid#159861PurposeThree-fragment Gateway compatible flourophore for expression in C. elegansDepositorInsert[1-2] ENTR - Fluor - mCardinal(1 intron, syntrons(3), NLS(2), no_atg, no_stop)
ExpressionWormMutationNot applicableAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKL2-138
Plasmid#219413PurposeEstradiol-inducible expression of 42-10 Zinc finger activatorDepositorInsert{pTEF1-ZEV}{pGal1(Zif268op)x6-3xFLAG-NLS-VP16-mRuby2-(42-10-4x)-PDZLigWT-tSSA1}
ExpressionYeastMutationFour Arg to Ala mutations in zinc finger backboneAvailable sinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only