We narrowed to 4,933 results for: puc

1. pTS1035

   Plasmid

   #169614

   Purpose

   Tier-2 vector encoding PmPGK1-driven secreted Nluc (PmPGK1-IgkSS-Nluc-pA::A2-pA::A3-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PPGK-driven expression of secreted Nluc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PPGK

   Available since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTS1032

   Plasmid

   #169612

   Purpose

   Tier-2 vector encoding PhCMV-driven secreted Nluc (A1-pA::PhCMV-IgkSS-Nluc-pA::A3-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven expression of secreted Nluc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTS1031

   Plasmid

   #169611

   Purpose

   Tier-2 vector encoding PhCMV-driven secreted Nluc (PhCMV-IgkSS-Nluc-pA::A2-pA::A3-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven expression of secreted Nluc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTS1033

   Plasmid

   #169613

   Purpose

   Tier-2 vector encoding PhCMV-driven secreted Nluc (A1-pA::A2-pA::PhCMV-IgkSS-Nluc-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven expression of secreted Nluc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTS1036

   Plasmid

   #169615

   Purpose

   Tier-2 vector encoding PmPGK1-driven secreted Nluc (A1-pA::PmPGK1-IgkSS-Nluc-pA::A3-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PPGK-driven expression of secreted Nluc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PPGK

   Available since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. FBXO7_Halo_C_allele

   Plasmid

   #178158

   Purpose

   Donor vector for endogenous tagging of human FBXO7 at the C-terminus with halotag

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Insert

   Halotag (FBXO7 Human)
   

   Use

   Donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. SETX_Halo_C_allele

   Plasmid

   #178169

   Purpose

   Donor vector for endogenous tagging of human SETX at the C-terminus with halotag

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Insert

   Halotag (SETX Human)
   

   Use

   Donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. ITM2B_Halo_C_allele

   Plasmid

   #178160

   Purpose

   Donor vector for endogenous tagging of human ITM2B at the C-terminus with halotag

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Insert

   Halotag (ITM2B Human)
   

   Use

   Donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GCH1_Halo_N_allele

   Plasmid

   #178159

   Purpose

   Donor vector for endogenous tagging of human GCH1 at the N-terminus with halotag

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Insert

   Halotag (GCH1 Human)
   

   Use

   Donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBL-2

    Plasmid

    #162769

    Purpose

    Minimal backbone with AmpR

    Depositor

    Ivan Vorobiev

    Article

    Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology; Molecular cloning

    Tags

    Expression

    Mutation

    Promoter

    no

    Available since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TC811

    Plasmid

    #164893

    Purpose

    TRE::YFP, inducible YFP expression when co-express with rtTA

    Depositor

    Tian Chi

    Article

    Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

    Insert

    YFP
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    April 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pISKP1-TIR1

    Plasmid

    #165061

    Purpose

    Integrative expression of SKP1-fusing rice auxin receptor gene OsTIR1

    Depositor

    Claudia Vickers

    Article

    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

    Insert

    P-URA3>KlURA3>T-AgTEF1- T-PGK1-P-ACS2> SKP1>OsTIR1opt>T-URA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    WT

    Promoter

    Available since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDest-313 (attR4-attR3)

    Plasmid

    #161898

    Purpose

    Multisite Gateway (attR4-attR3) vector for mammalian protein expression

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pILGFP105

    Plasmid

    #165060

    Purpose

    Integrative expression of nucleus-targeting yEGFP-AID*(mini auxin inducible degron)-CUP1

    Depositor

    Claudia Vickers

    Article

    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

    Insert

    P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>SV40NSL>T-PGK1- T-URA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    WT

    Promoter

    Available since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pILGFP104

    Plasmid

    #165059

    Purpose

    Integrative expression of yEGFP-AID*(mini auxin inducible degron)-CUP1

    Depositor

    Claudia Vickers

    Article

    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

    Insert

    P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>T-PGK1-T-URA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    WT

    Promoter

    Available since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJAID9L104

    Plasmid

    #165054

    Purpose

    Integrative expression of yEGFP-AID*-CUP1 and rice auxin receptor gene OsTIR1.

    Depositor

    Claudia Vickers

    Article

    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

    Insert

    P-URA3>KlURA3>T-AgTEF1-P-TEF1>yEGFP>AID*>CUP1>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    WT

    Promoter

    Available since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMC_cherry donor_Rosa target (pE002)

    Plasmid

    #149348

    Purpose

    Replace donor vector for cherry insertion using the mouse Rosa26 guide RNA

    Depositor

    Ralf Kuehn

    Article

    Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

    Insert

    cherry
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMC_cherry donor_Polb target (pE038)

    Plasmid

    #149349

    Purpose

    Replace donor vector for cherry insertion using the human Polb guide RNA

    Depositor

    Ralf Kuehn

    Article

    Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

    Insert

    cherry
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEPQDKN0332

    Plasmid

    #162314

    Purpose

    Reporter plasmid for TNT SP6 Coupled Wheat Germ Extract System with detection via HiBiT

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    SP6prom-EO1-sfGFP-HiBiT
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEPQDKN0329

    Plasmid

    #162315

    Purpose

    TEV protease plasmid for T7-driven E. coli cell-free protein synthesis with detection via HiBiT

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    T7prom-RBS-NET-TEVprotease-HiBiT
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge