Addgene

We narrowed to 75,672 results for: ELL

Showing: 2841 - 2860 of 75672 results

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Perturb-FISH

Plasmid

#220626

Purpose

expression of gRNAs under U6T7 promoter

Depositor

Samouil Farhi

Article

Binan et al Cell. 2025 Mar 10:S0092-8674(25)00197-7.

Insert

sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

BsmBI

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

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pHR-PD-L1-SNAP

Plasmid

#223577

Purpose

Lentiviral expression of human PD-L1 with SNAP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Cell Rep. 2018 Jul 10;24(2):379-390.e6.

Insert

PD-L1 (CD274 Human)

Use

Lentiviral; mammalian expression

Tags

SNAP

Expression

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CLIP-PD-L1

Plasmid

#223571

Purpose

Lentiviral expression of human PD-L1 with CLIP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Cell Rep. 2018 Jul 10;24(2):379-390.e6.

Insert

PD-L1 (CD274 Human)

Use

Lentiviral; mammalian expression

Tags

CLIP

Expression

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pMBA332

Plasmid

#214744

Purpose

Low copy thyA-infA plasmid containing gfp and no antibiotic resistance gene (ARG)

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23119-RiboJ-RBS(21992)-gfpmut3

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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pMBA337

Plasmid

#214750

Purpose

Medium copy thyA-infA plasmid containing bicistronic gfp-specR for use in mouse work validation

Depositor

Tae Seok Moon

Article

Amrofell et al Cell Rep Methods. 2023 Dec 18;3(12):1006

Insert

BBa_J23119-RiboJ-RBS(21992)-gfpmut3-RBS(4533)-specR

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+N-eGFP_SAMD9L_Asn885fs

Plasmid

#206175

Purpose

mammalian expression of SAMD9L Asn885fs

Depositor

Christopher Goodnow

Article

Russell et al Proc Natl Acad Sci U S A. 2021 Aug 24;11

Insert

SAMD9L (SAMD9L Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

N885Tfs*6

Promoter

CMV

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+N-eGFP_SAMD9L_Lys878fs

Plasmid

#206174

Purpose

mammalian expression of SAMD9L Lys878fs

Depositor

Christopher Goodnow

Article

Russell et al Proc Natl Acad Sci U S A. 2021 Aug 24;11

Insert

SAMD9L (SAMD9L Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

K878Sfs*13

Promoter

CMV

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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mPapaya-Rab4a-7

Plasmid

#56645

Purpose

Localization: Ras GTPase, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

Rab4a (RAB4A Human)

Use

Tags

mPapaya

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-SARS2SΔC-H2gp41

Plasmid

#170389

Purpose

Generation of SARS-CoV-2 spike pseudotyped lentivirus

Depositor

Nir Hacohen

Article

Filbin et al Cell Rep Med. 2021 May 18;2(5):100287. d

Insert

SARS-CoV-2 Spike (S SARS-CoV-2)

Use

Tags

HIV gp41

Expression

Mammalian

Mutation

Deletion of C terminal and fusion with H2 from HI…

Promoter

CMV

Available since

June 23, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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MSCV-N MCV ER

Plasmid

#37860

Depositor

Karl Munger

Article

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

MCV ER (MCPyV_gp2, MCPyV_gp1 Human Merkel Cell Virus)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

PKG

Available since

July 24, 2012

Availability

Academic Institutions and Nonprofits only
pLV-CMV-LoxP-DsRed-LoxP-eGFP

Plasmid

#65726

Purpose

Functions as a genetic Cre reporter that switches from DsRed expression to eGFP expression upon presence of Cre recombinase.

Depositor

Jacco van Rheenen

Article

Zomer et al Cell. 2015 May 21;161(5):1046-57. doi: 1

Insert

LoxP-DsRed-LoxP-eGFP

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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LBR EGFP

Plasmid

#128150

Purpose

Expression of lamin B receptor

Depositor

Jennifer Lippincott-Schwartz

Article

Ellenberg et al J Cell Biol. 1997 Sep 22. 138(6):1193-20

Insert

lamin B receptor (LBR Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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H2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen

Plasmid

#129631

Purpose

Visualization of Akt and ERK activity

Depositor

Dorus Gadella

Article

Chavez-Abiega et al J Cell Sci. 2022 Mar 15;135(6):jcs259685

Insert

H2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCF1148

Plasmid

#222330

Purpose

Fluorescent reporter plasmid for Veillonella

Depositor

J Christopher Fenno

Article

Goetting-Minesky et al Lett Appl Microbiol. 2024 Aug 5;77(8):ov

Insert

bs2 gene with Veillonella parvula SKV38 mdh promoter

Use

E. coli-veillonella shuttle plasmid

Tags

Expression

Bacterial

Mutation

Promoter

Veillonella parvula SKV38 mdh promoter

Available since

Oct. 31, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pEND-349_Cacnes_ori

Plasmid

#225604

Purpose

Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector with C. acnes chloramphenicol resistance, harbouring a medium-copy pBR322 E. coli ori (no ROP)

Depositor

Marc Güell

Article

Guillermo et al Cell Systems, 2025

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pEND-400_Cacnes_CAMP2

Plasmid

#225606

Purpose

Suicide C. acnes vector derivative of pEND-350 optimized for recombination into the CAMP2 locus, with BxbI recombination sites flanking EryR resistance cassette

Depositor

Marc Güell

Article

Guillermo et al Cell Systems, 2025

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pPPI4-Strep-SNAP-PD-1

Plasmid

#223569

Purpose

Protein purification of human PD-1 with Twin-Strep and SNAP tags from mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Cell Rep. 2018 Jul 10;24(2):379-390.e6.

Insert

PD-1 (PDCD1 Human)

Use

Tags

Twin-Strep, SNAP

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA-FLAG-METTL3

Plasmid

#160250

Purpose

Express FLAG-METTL3 in human cells

Depositor

Alessandro Fatica

Article

Sorci et al Cell Death Dis. 2018 Jul 23;9(8):796. do

Insert

methyltransferase like 3 (METTL3 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pEND-418_Cacnes_LysA

Plasmid

#225607

Purpose

Suicide C. acnes vector to generate LysA (PPA_RS06345) deletion leaving an Ery resistance cassette flanked by BxbI recombination sites

Depositor

Marc Güell

Article

Guillermo et al Cell Systems, 2025

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pCambia2300 - ProRUBY:RUBY-Citrine

Plasmid

#128796

Purpose

Fluorescent reporter for subcellular localisation and expression analysis of RUBY

Depositor

George Haughn

Article

Sola et al Plant Cell. 2019 Apr;31(4):809-831. doi:

Insert

RUBY PARTICLES IN MUCILAGE (AT1G19900 Mustard Weed)

Use

Tags

Citrine

Expression

Plant

Mutation

Promoter

ProRUBY

Available since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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