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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3,035 results for: ER;

Showing: 2841 - 2860 of 3035 results

  1. pBiex PKC - Peptide 8 mCit mCer

    Plasmid
    #83563
    Purpose
    Expresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.
    Depositor
    Sivaraj Sivaramakrishnan
    Article
    Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.
    Insert
    PRKCA (PRKCA Human)
    Tags
    10 nm ER/K Helix, FLAG purification tag, TEV Prot…
    Expression
    Bacterial and Insect
    Mutation
    Only catalytic domain of PKC (aa 335-672)
    Promoter
    T7 Promoter
    Available Since
    Nov. 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    83563-plasmid-map-sequence-id-159470

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  4. EC71167

    Plasmid
    #154066
    Purpose
    Level 1 Position 3 Golden Gate vector, pL1M-R3-UbqP-Loxp-ER-Targ-mCherry-HDEL-Loxp-eGFP
    Depositor
    Cristobal Uauy
    Article
    Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:
    Insert
    ZmUBI::lox-mCHERRY-lox-T35S-eGFP-TAct
    Use
    Synthetic Biology
    Mutation
    All BsaI, Esp3I and BPiI restriction sites were r…
    Promoter
    Zea mays Ubiquitin Promoter
    Available Since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    154066-plasmid-map-sequence-id-312954

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  6. pRS405 SEC66-VAC8-RFP

    Plasmid
    #166843
    Purpose
    Expresses ER localized Vac8-RFP in yeasts. Made replacing the first 21 base pairs of the VAC8 ORF with the first 162 base pairs from the SEC66 ORF, which encode the Sec66 transmembrane domain.
    Depositor
    Daniel Klionsky
    Article
    Gatica et al Autophagy. 2020 Jun 8. doi: 10.1080/1554
    Insert
    VAC8 (VAC8, SEC66 Budding Yeast)
    Tags
    RFP and SEC66 transmembrane domain
    Expression
    Yeast
    Mutation
    SEC66 transmembrane domain
    Promoter
    VAC8 promoter
    Available Since
    April 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    166843-plasmid-map-sequence-id-329331

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  14. pBiex PKC - Peptide 14 mCit mCer

    Plasmid
    #83565
    Purpose
    Expresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the pseudosubstrate of PKCalpha.
    Depositor
    Sivaraj Sivaramakrishnan
    Article
    Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.
    Insert
    PKC alpha (PRKCA Human)
    Tags
    10 nm ER/K Helix, FLAG Purificaiton Tag, Tev Prot…
    Expression
    Bacterial and Insect
    Mutation
    Only catalytic domain of PKC (aa 335-672)
    Promoter
    T7 Promoter
    Available Since
    Nov. 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    83565-plasmid-map-sequence-id-159510

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  18. XZ363 EF1a-VEGFR1sp-3xFlag-VEGFR1(ecto+TM)-TS-ADAR2dd(QA)-export

    Plasmid
    #233382
    Purpose
    Optimized VEGF-sensing eLIDAR with VEGFR1 ecto+TM domains fused with ADAR2dd, with Golgi traffic sequence and ER export sequence
    Depositor
    Xiaojing Gao
    Article
    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038
    Insert
    VEGFR1(ecto+TM)-ADAR2dd
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    E488Q, T501A on ADAR2dd
    Promoter
    EF1a
    Available Since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    233382-plasmid-map-sequence-id-466380

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  19. XZ375 EF1a-IL2Rbsp-3xFLAG-IL2Rb(ECD+TM)-TS-ADAR2dd-export

    Plasmid
    #233386
    Purpose
    Optimized IL-2-sensing eLIDAR with IL-2 beta chain ecto+TM domains fused with ADAR2dd, with Golgi traffic sequence and ER export sequence
    Depositor
    Xiaojing Gao
    Article
    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038
    Insert
    IL2Rb(ecto+TM)-ADAR2dd
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    E488Q, T501A on ADAR2dd
    Promoter
    EF1a
    Available Since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    233386-plasmid-map-sequence-id-466377

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Showing: 2841 - 2860 of 3035 results