We narrowed to 88,001 results for: MAL

1. [Bla][IRES][GFP]

   Plasmid

   #97193

   Purpose

   Vector for our translation quantification assay (Fig. 5). Double reporter vector with internal ribosome entry site.

   Depositor

   Edward Boyden

   Article

   Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11

   Inserts

   beta-Lactamase
   GFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLEX-uORF-HA-birA*-STOP-IRES-Puro

   Plasmid

   #120558

   Purpose

   Expresses control HA-birA* in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   birA
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   R118G

   Promoter

   CMV

   Available Since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCI-neo-μ1A-(HA)3

   Plasmid

   #198177

   Purpose

   Expression of HA-tagged AP-1 μ1A in mammalian cells

   Depositor

   Juan Bonifacino

   Article

   Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

   Insert

   AP-1 μ1A
   

   Use

   Tags

   triple HA tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. Flag-mPer2ER-pBABEpuro

   Plasmid

   #61430

   Purpose

   Express mPER2-ER* fusion protein

   Depositor

   Aziz Sancar

   Article

   Ye et al Genes Dev. 2014 Sep 15;28(18):1989-98. d

   Insert

   mPER2 (Per2 Mouse)
   

   Use

   Retroviral

   Tags

   Flag and mouse estrogen receptor ligand binding d…

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pMET7-GAG-GW

   Plasmid

   #194634

   Purpose

   Gateway destination vector for mammalian expression of Virotrap bait constructs

   Depositor

   Sven Eyckerman

   Article

   Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

   Insert

   p55 GAG
   

   Use

   Tags

   PQS1

   Expression

   Mammalian

   Mutation

   Promoter

   SRalpha

   Available Since

   Jan. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-S9

   Plasmid

   #132948

   Purpose

   Expresses N-terminal 6xHis-tagged S9 ribosomal protein in E.coli

   Depositor

   James Williamson

   Article

   Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

   Insert

   30S ribosomal subunit protein S9 (rpsI E.coli)
   

   Use

   Tags

   N-terminal 6xHis-tag

   Expression

   Bacterial

   Mutation

   E52C

   Promoter

   T7

   Available Since

   May 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTS94 pHAGE2 CMVtetO2 GFP-22xGS-3C-MCS

   Plasmid

   #199347

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-3C-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EGFP-3C

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. p458 VRER

   Plasmid

   #101728

   Purpose

   Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 VRER
   

   Use

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   VRER (D1135V, G1218R, R1335E, and T1337R mutation…

   Promoter

   Available Since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA5/FRT/TO/Flag-tGFP-HuR.wt

   Plasmid

   #110376

   Purpose

   Mammalian expression of turboGFP-HuR (wild type) with FLAG tag, Flp-In T-Rex system

   Depositor

   Sandra Martha Gomes Dias

   Insert

   ELAV like RNA binding protein 1 (ELAVL1 Human)
   

   Use

   Tags

   FLAG and tGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. p330 VQR

    Plasmid

    #101730

    Purpose

    Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VQR
    

    Use

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VQR (D1135V, R1335Q, and T1337R mutations in Cas9)

    Promoter

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMRXIP-Myc-YKT6

    Plasmid

    #116945

    Purpose

    Expressing Myc-YKT6 for mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Matsui et al J Cell Biol. 2018 Aug 6;217(8):2633-2645

    Insert

    Synaptobrevin homolog YKT6 (YKT6 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKF-P14MM2AG5h

    Plasmid

    #128254

    Purpose

    Mammalian positive feedback synthetic gene circuit within a Flp-In expression vector.

    Depositor

    Gabor Balazsi

    Article

    Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:

    Insert

    rtTA-Advanced::P2A::EGFP
    

    Use

    Synthetic Biology; Flp-in expression vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tight TRE promoter

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. NKP64 GFP (1007)

    Plasmid

    #62020

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Nucleus. 2012 Nov-Dec;3(6):552-64. doi:

    Insert

    SLC38A10 (SLC38A10 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

14. 4xHRE_minSV40-sfGFP-CMV_dsRed

    Plasmid

    #117400

    Purpose

    sfGFP expressed from hypoxia-inducible minimal SV40 promoter and dsRed expressed from consitutive CMV promoter

    Depositor

    Yvonne Chen

    Article

    Ede et al ACS Synth Biol. 2016 May 20;5(5):395-404

    Insert

    SuperFolder GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA 3.1 (+) Tacδ

    Plasmid

    #81177

    Purpose

    Mammalian expression of human Tacδ (GFFKR cytoplasmic tail)

    Depositor

    Chinten James Lim

    Article

    Liu et al Mol Cell Biol. 2013 Nov;33(21):4334-45.

    Insert

    Tacδ
    

    Use

    Tags

    KLGFFKR

    Expression

    Mammalian

    Mutation

    CD25 amino acids 1 to 263 fused to KLGFFKR cytopl…

    Promoter

    CMV

    Available Since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNICE HA-mSYD1A

    Plasmid

    #59361

    Purpose

    Expression plasmid for HA-tagged mSYD1A / Syde1 (HA-tag at N-terminus of mSYD1A)

    Depositor

    Peter Scheiffele

    Article

    Wentzel et al Neuron. 2013 Jun 19;78(6):1012-23. doi:

    Insert

    mSYD1A (Syde1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEF-1α_ZF9LOV[C2]-VP64 (pLZA126)

    Plasmid

    #203911

    Purpose

    Encodes engineered opto-ZF9 with AsLOV2(408-543) inserted in C2 position in mammalian cells

    Depositor

    Jared Toettcher

    Article

    Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

    Insert

    ZF9LOV[C2]-VP64
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available Since

    July 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEF-1α_ZF9LOV[C1]-VP64 (pLZA125)

    Plasmid

    #203910

    Purpose

    Encodes engineered opto-ZF9 with AsLOV2(408-543) inserted in C1 position in mammalian cells

    Depositor

    Jared Toettcher

    Article

    Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

    Insert

    ZF9LOV[C1]-VP64
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available Since

    July 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAGEN-SBP-DICER1

    Plasmid

    #50558

    Purpose

    purification of Dicer1 from mammalian cells

    Depositor

    Yukihide Tomari

    Article

    Betancur et al RNA. 2012 Jan;18(1):24-30. doi: 10.1261/

    Insert

    Dicer1 (Dicer1 Mouse)
    

    Use

    Tags

    SBP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Jan. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

20. SlugBE4max

    Plasmid

    #163799

    Purpose

    Expresses AncBE4max and nSlugCas9

    Depositor

    Yongming Wang

    Article

    Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    SlugCas9 (D10A)

    Promoter

    Available Since

    April 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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