We narrowed to 88,163 results for: Mal

1. pRSF-1b-S14

   Plasmid

   #128596

   Purpose

   Expresses tagless 30S ribosomal subunit protein S14 in E.coli

   Depositor

   James Williamson

   Article

   Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

   Insert

   30S ribosomal subunit protein S14 (rpsN E.coli)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti6.3-efSaCas9-BlastR

   Plasmid

   #220971

   Purpose

   Expresses human codon-optimized efSaCas9

   Depositor

   Hyongbum Kim

   Article

   Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

   Insert

   efSaCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. M-SPn-VP64

   Plasmid

   #48674

   Purpose

   Mammalian SP-VP64 nuclease-null Cas9 activator expression, human optimized

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Insert

   Cas9-VP64, nuclease-null
   

   Use

   CRISPR

   Tags

   NLS and VP64

   Expression

   Mutation

   nuclease-null

   Promoter

   CMV

   Available Since

   Dec. 9, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pGH156_MS2-AID-Hygro

   Plasmid

   #85415

   Purpose

   lentiviral expression vector for MS2-AID fusion protein with hygromycin B selectable marker

   Depositor

   Michael Bassik

   Article

   Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   MS2-AID and Hygromycin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. px330 VRER

   Plasmid

   #101729

   Purpose

   Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 VRER
   

   Use

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   VRER (D1135V, G1218R, R1335E, and T1337R mutation…

   Promoter

   Available Since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. [Bla][IRES][GFP]

   Plasmid

   #97193

   Purpose

   Vector for our translation quantification assay (Fig. 5). Double reporter vector with internal ribosome entry site.

   Depositor

   Edward Boyden

   Article

   Adamala et al Proc Natl Acad Sci U S A. 2016 May 10;11

   Inserts

   beta-Lactamase
   GFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMET7-GAG-HRAS

   Plasmid

   #80604

   Purpose

   Mammalian expression vector that expresses the HIV-1 GAG protein with HRAS fused to it

   Depositor

   Sven Eyckerman

   Article

   Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

   Insert

   HIV-1 p55 GAG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SRalpha

   Available Since

   Aug. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLEX-uORF-HA-birA*-STOP-IRES-Puro

   Plasmid

   #120558

   Purpose

   Expresses control HA-birA* in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   birA
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   R118G

   Promoter

   CMV

   Available Since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCI-neo-μ1A-(HA)3

   Plasmid

   #198177

   Purpose

   Expression of HA-tagged AP-1 μ1A in mammalian cells

   Depositor

   Juan Bonifacino

   Article

   Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

   Insert

   AP-1 μ1A
   

   Use

   Tags

   triple HA tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. Flag-mPer2ER-pBABEpuro

    Plasmid

    #61430

    Purpose

    Express mPER2-ER* fusion protein

    Depositor

    Aziz Sancar

    Article

    Ye et al Genes Dev. 2014 Sep 15;28(18):1989-98. d

    Insert

    mPER2 (Per2 Mouse)
    

    Use

    Retroviral

    Tags

    Flag and mouse estrogen receptor ligand binding d…

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pMET7-GAG-GW

    Plasmid

    #194634

    Purpose

    Gateway destination vector for mammalian expression of Virotrap bait constructs

    Depositor

    Sven Eyckerman

    Article

    Eyckerman et al Nat Commun. 2016 Apr 28;7:11416. doi: 10

    Insert

    p55 GAG
    

    Use

    Tags

    PQS1

    Expression

    Mammalian

    Mutation

    Promoter

    SRalpha

    Available Since

    Jan. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-S9

    Plasmid

    #132948

    Purpose

    Expresses N-terminal 6xHis-tagged S9 ribosomal protein in E.coli

    Depositor

    James Williamson

    Article

    Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

    Insert

    30S ribosomal subunit protein S9 (rpsI E.coli)
    

    Use

    Tags

    N-terminal 6xHis-tag

    Expression

    Bacterial

    Mutation

    E52C

    Promoter

    T7

    Available Since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTS94 pHAGE2 CMVtetO2 GFP-22xGS-3C-MCS

    Plasmid

    #199347

    Purpose

    Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-3C-tagged protein in mammalian cells.

    Depositor

    Rebecca Voorhees

    Article

    Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP-3C

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available Since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. p458 VRER

    Plasmid

    #101728

    Purpose

    Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VRER
    

    Use

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VRER (D1135V, G1218R, R1335E, and T1337R mutation…

    Promoter

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA5/FRT/TO/Flag-tGFP-HuR.wt

    Plasmid

    #110376

    Purpose

    Mammalian expression of turboGFP-HuR (wild type) with FLAG tag, Flp-In T-Rex system

    Depositor

    Sandra Martha Gomes Dias

    Insert

    ELAV like RNA binding protein 1 (ELAVL1 Human)
    

    Use

    Tags

    FLAG and tGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. p330 VQR

    Plasmid

    #101730

    Purpose

    Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VQR
    

    Use

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VQR (D1135V, R1335Q, and T1337R mutations in Cas9)

    Promoter

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMRXIP-Myc-YKT6

    Plasmid

    #116945

    Purpose

    Expressing Myc-YKT6 for mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Matsui et al J Cell Biol. 2018 Aug 6;217(8):2633-2645

    Insert

    Synaptobrevin homolog YKT6 (YKT6 Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. NKP64 GFP (1007)

    Plasmid

    #62020

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Korfali et al Nucleus. 2012 Nov-Dec;3(6):552-64. doi:

    Insert

    SLC38A10 (SLC38A10 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pKF-P14MM2AG5h

    Plasmid

    #128254

    Purpose

    Mammalian positive feedback synthetic gene circuit within a Flp-In expression vector.

    Depositor

    Gabor Balazsi

    Article

    Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:

    Insert

    rtTA-Advanced::P2A::EGFP
    

    Use

    Synthetic Biology; Flp-in expression vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tight TRE promoter

    Available Since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. 4xHRE_minSV40-sfGFP-CMV_dsRed

    Plasmid

    #117400

    Purpose

    sfGFP expressed from hypoxia-inducible minimal SV40 promoter and dsRed expressed from consitutive CMV promoter

    Depositor

    Yvonne Chen

    Article

    Ede et al ACS Synth Biol. 2016 May 20;5(5):395-404

    Insert

    SuperFolder GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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