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pLEX-FKBP-Rab7a-Blasticidin

Plasmid

#120716

Purpose

Expresses late endosome-targeted recruiter protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

Rab7a

Use

Lentiviral

Tags

FKBP and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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P628_SpCas9-RecA

Plasmid

#87263

Purpose

Human codon optimized RecA protein was fused to SpCas9 for enhanced genome editing efficiency

Depositor

Yonglun Luo

Article

Lin et al J Biotechnol. 2017 Mar 1;247:42-49. doi:

Insert

RecA

Use

CRISPR

Tags

FLAG

Expression

Mammalian

Mutation

no

Promoter

CBh

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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P823_eSpCas9(1.1)-RecA

Plasmid

#87264

Purpose

Human codon optimized RecA protein was fused to eSpCas9(1.1) for enhanced genome editing efficiency

Depositor

Yonglun Luo

Article

Lin et al J Biotechnol. 2017 Mar 1;247:42-49. doi:

Insert

RecA

Tags

FLAG

Expression

Mammalian

Promoter

CBh

Available Since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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HA-UBE2E1 C131A in pcDNA3.1

Plasmid

#118214

Purpose

Expresses HA-UBE2E1 C131A catalytic mutant in mammalian cells

Depositor

Cynthia Wolberger

Article

Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

Insert

UBE2E1 (UBE2E1 Human)

Tags

HA

Expression

Mammalian

Mutation

changed cysteine 131 to alanine

Available Since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pTP924 pHAGE2 CMV GFP

Plasmid

#199392

Purpose

Lentiviral transfer plasmid for constitutive expression of a GFP in mammalian cells

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Insert

GFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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pTS103 pHAGE2 CMVtetO2 MCS-22xGS-GFP

Plasmid

#199356

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally GFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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GWF058

Plasmid

#133609

Purpose

UAS-mCherry/CMV-Gal4DBD-NS3a-P2a-DNCR2-VPR

Depositor

Dustin Maly

Article

Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038

Insert

Gal4DBD-NS3a, DNCR2-VPR

Expression

Mammalian

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pPD1028 ZF43x6-Compact(SV40Min)_EYFP

Plasmid

#138912

Purpose

EYFP reporter vector for the ZF43x6-Compact(overlapping ZF37) promoter with a SV40 minimal promoter

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Insert

ZF43x6-Compact(SV40min)

Use

Synthetic Biology

Expression

Mammalian

Promoter

ZF43x6-Compact(SV40min)

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-BRAT1

Plasmid

#62802

Purpose

Expresses human BRAT1 with an N-terminal HA tag in mammalian cells

Depositor

Toru Ouchi

Article

So et al BMC Cancer. 2014 Jul 29;14:548. doi: 10.

Insert

BRAT1 (BRAT1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only
pAAV-E1.1-RFP

Plasmid

#22928

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 Nov 9;3:19.

Insert

E1.1 binding site from Scardigli et al., 2003 with minimal CMV

Use

AAV

Expression

Mammalian

Available Since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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pRK-flag-USP19 C506S

Plasmid

#78584

Purpose

Expression flag-USP19 C506S in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-USP19 C506S (USP19 Human)

Tags

flag

Expression

Mammalian

Mutation

mutate 506 cysteine residue to serine

Promoter

CMV

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
pMiniP

Plasmid

#118049

Purpose

GBPart - Minimal Promoter for expression in mammalian cells

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

minimal TATA-box promoter with low basal activity

Use

Synthetic Biology

Expression

Mammalian

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti6.3-SauriCas9-BlastR

Plasmid

#220967

Purpose

Expresses human codon-optimized SauriCas9

Depositor

Hyongbum Kim

Article

Seo et al Nat Methods. 2023 Jul;20(7):999-1009. do

Insert

SauriCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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px458 EQR

Plasmid

#101731

Purpose

Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

SpCas9 EQR

Tags

3xFLAG-NLS and NLS

Expression

Mammalian

Mutation

EQR (D1135E, R1335Q, and T1337R mutations in Cas9)

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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Ef1a_NANOG_Ires_Puro

Plasmid

#18920

Depositor

Linzhao Cheng

Article

Mali et al Stem Cells. 2008 Aug . 26(8):1998-2005.

Insert

NANOG (NANOG Human)

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

Sept. 4, 2008

Availability

Academic Institutions and Nonprofits only
pAAV-SMVP-Cas9N-U6-gRNA P2

Plasmid

#80944

Purpose

Expresses Cas9N in mammalian cells; expresses gRNA P2 for Pd-l1 binding

Depositor

George Church

Article

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9N

Use

AAV, CRISPR, and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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px459 VRER

Plasmid

#101716

Purpose

sgRNA/SpCas9 expression plasmid with Cas9 VRER mutations (NGCG PAM)

Depositor

Andrew Holland

Article

Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

Insert

SpCas9 VRER

Tags

3xFLAG-NLS and NLS

Expression

Mammalian

Mutation

VRER (D1135V, G1218R, R1335E and T1337R)

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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GWF297

Plasmid

#133612

Purpose

TET3G-2xMS2(wt+f6), NLS-MCP-ANR-ANR-P2a-BFP

Depositor

Dustin Maly

Article

Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038

Insert

TET3G-2xMS2(wt+f6), MCP-ANR-ANR, BFP

Expression

Mammalian

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pXPR_212

Plasmid

#133457

Purpose

U6 promoter expresses customizable Spyo-guide; EFS promoter expresses SaurCas9 and 2A site provides puromycin resistance

Depositor

John Doench

Article

DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

Insert

control guide

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Oct. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pMS2CP(V29I)-myc-T2A-tagRFP

Plasmid

#140260

Purpose

Expresses MS2CP(V29I) in mammalian cells

Depositor

Hirohide Saito

Article

Ono et al ACS Synth Biol. 2020 Jan 17;9(1):169-174

Insert

Coat protein of bacteriphage MS2

Use

Synthetic Biology

Tags

myc-T2A-tagRFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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