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Showing: 2841 - 2860 of 76964 results

  1. pcDNA-rUSP19CYT-siRNAresistant

    Plasmid
    #155252
    Purpose
    for mammalian overexpression of the siRNA resistant CYT isoform of rat USP19
    Depositor
    Simon Wing
    Insert
    USP19 (CYT isoform) siRNA resistant (Usp19 Rat)
    Use
    Tags
    6XHis-3XFlag
    Expression
    Mammalian
    Mutation
    Lys(23)AAG to AAA, Lys(24)AAG to AAA, Gln(26)CAG …
    Promoter
    CMV
    Available since
    Oct. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    155252-plasmid-map-sequence-id-313955

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  2. pJK139

    Plasmid
    #73315
    Purpose
    Pas2p peroxisomal membrane biogenesis protein
    Depositor
    T. J. Kappock
    Insert
    Pas2p peroxisomal membrane targeting protein
    Use
    Tags
    His6 and c-myc
    Expression
    Yeast
    Mutation
    Encodes a glycine-204 to serine mutation
    Promoter
    AOX1
    Available since
    March 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73315-plasmid-map-sequence-id-140431

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  3. OMM-SPLICSs-nFAST

    Plasmid
    #210430
    Purpose
    Expresses outer mitochondria-targeted split-NFAST for Short-range ER-Mito Contact sites
    Depositor
    Tito Calì
    Insert
    OMM-SPLICSs-nFAST
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    210430-plasmid-map-sequence-id-415625

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  5. pSecUAG_UTu1D

    Plasmid
    #233867
    Purpose
    Expresses machinery necessary for selenocysteine incorporation at UAG codons in BL21-derived cells, spectinomycin resistant
    Depositor
    Dieter Söll, Natalie Krahn
    Inserts
    allo-tRNA UTu1D
    thioredoxin
    Selenophosphate synthase
    Selenocysteine synthase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Cysteine 32 switched to Selenocysteine (UAG) and …
    Promoter
    EM7, araBAD, and native As SelD
    Available since
    May 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    233867-plasmid-map-sequence-id-471093

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  6. ELL3 Streptag CtoA

    Plasmid
    #127283
    Purpose
    Expresses mouse ELL3 with streptactin tag; To interrogate if the mutation decreases putative Myc activity
    Depositor
    Christine Milcarek
    Insert
    ELL3 (NM_145973) (ELL3 Mouse)
    Use
    Tags
    Twin strep-tag
    Expression
    Mammalian
    Mutation
    CEYLHNK to AEYLHQK
    Promoter
    EF1a
    Available since
    Aug. 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    127283-plasmid-map-sequence-id-250049

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  9. SARS-CoV2 Nucleocapsid

    Plasmid
    #153201
    Purpose
    Expresses myc-tagged nucleocapsid (N) from SARS-CoV2 in mammalian cells
    Depositor
    Peter Klein
    Article
    Liu et al Proc Natl Acad Sci U S A. 2021 Oct 19;11
    Insert
    Nucleocapsid from SARS-CoV-2 (N severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2))
    Use
    Tags
    Myc tag (5X)
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 15, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    153201-plasmid-map-sequence-id-305776

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  12. LV-AR-KKKAAA

    Plasmid
    #171219
    Purpose
    Lenti-viral expression of human androgen receptor DNA-binding mutant: K630A-K632A-K633A, mutations at the boundary between AR DNA-binding domain and hinge domain.
    Depositor
    Steven Balk
    Article
    Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:
    Insert
    AR-KKKAAA (AR Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    AR-K630A-K632A-K633A
    Promoter
    EF-1a
    Available since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    171219-plasmid-map-sequence-id-342535

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  13. LV-AR-C563A-C596A

    Plasmid
    #171220
    Purpose
    Lenti-viral expression of human androgen receptor DNA-binding mutant: mutations of one essential cysteine on each of the two zinc fingers.
    Depositor
    Steven Balk
    Article
    Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:
    Insert
    AR-C563A-C596A (AR Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    AR-C563A-C596A
    Promoter
    EF-1a
    Available since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    171220-plasmid-map-sequence-id-342449

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  14. LV-AR-R599A-N600A

    Plasmid
    #171221
    Purpose
    Lenti-viral expression of human androgen receptor DNA-binding mutant: R599A-N600A, mutations in the D-box of the Zinc finger 2 that mediates AR dimerization.
    Depositor
    Steven Balk
    Article
    Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:
    Insert
    AR-R599A-N600A (AR Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    AR-R599A-N600A
    Promoter
    EF-1a
    Available since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    171221-plasmid-map-sequence-id-342450

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  15. LV-AR-AAFF

    Plasmid
    #171218
    Purpose
    Lenti-viral expression of human androgen receptor DNA-binding mutant: K580A-V581A, mutations in the P-box of the zinc finger 1 at the KVFF motif.
    Depositor
    Steven Balk
    Article
    Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:
    Insert
    AR-AAFF (AR Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    AR-K580A-V581A
    Promoter
    EF-1a
    Available since
    Sept. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    171218-plasmid-map-sequence-id-342447

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  16. Ath-HEN1::T2A::mCherry::TY1::H2B-pCFJ350-ttTi5605

    Plasmid
    #108362
    Purpose
    Expression of 2xFlag MYC tagged A.thaliana-HEN1 (Ath-HEN1, codon optimised for C. elegans) and mCherry under the promoter of choice in C. elegans
    Depositor
    Luisa Cochella
    Article
    Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461
    Inserts
    Ath-HEN1 (HEN1 Codon optimised for C. elegans, Mustard Weed)
    mCherry
    his-15 (his-15 Nematode)
    unc-54 3' UTR
    Use
    Tags
    2xFlag, MYC, T2A peptide, and TY1 linker
    Expression
    Worm
    Mutation
    Promoter
    A rab-3:Ath-HEN1:unc-54 3' UTR was cloned as…
    Available since
    May 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    108362-plasmid-map-sequence-id-209815

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  18. pLXSN p110 CUX1

    Plasmid
    #90471
    Purpose
    Retroviral vector expressing human p110 CUX1 (amino acids 747-1505) with a Myc and HA tag at the N- and C-terminue, respectively
    Depositor
    Alain Nepveu
    Article
    Kedinger et al J Biol Chem. 2009 Oct 2;284(40):27701-11
    Insert
    CUX1 (amino acids 747-1505) (CUX1 Human)
    Use
    Retroviral
    Tags
    HA and Myc
    Expression
    Mammalian
    Mutation
    Promoter
    Moloney murine leukemia virus long terminal repeat
    Available since
    March 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    90471-plasmid-map-sequence-id-205963

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  20. pT2iApowriter2(#336)

    Plasmid
    #184104
    Purpose
    cyclofen-inducible apoptosis activation and imaging (tEosFP) in zebrafish transient transgenic
    Depositor
    Sophie Vriz
    Article
    Feng et al Rep Prog Phys. 2013 Jul;76(7):072601. do
    Insert
    myr-DIAP1-5myc-tEosFP-nls'-P2A-Casp9-ERT2
    Use
    Zebrafish transient transgenesis
    Tags
    internal tag: 5myc
    Expression
    Mutation
    DIAP1: deleted 1-2, N19G, N20V, deleted 146-438; …
    Promoter
    Zf-Ubi
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    184104-plasmid-map-sequence-id-364017

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Showing: 2841 - 2860 of 76964 results