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We narrowed to 77,447 results for: Rest

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Showing: 2841 - 2860 of 77447 results

  3. pTet-On-TAP

    Plasmid
    #59017
    Purpose
    Construct allows tetracycline inducible expression of a protein of interest fused to a tandem affinity purification tag (TAP) in toxoplasma gondii.
    Depositor
    David Sibley
    Article
    Etheridge et al Cell Host Microbe. 2014 May 14;15(5):537
    Type
    Empty backbone
    Use
    Toxoplasma expression
    Tags
    Calmodulin Binding Peptide fused with a TEV Cleav…
    Promoter
    RPS13 with Tet Operators Inserted
    Available Since
    Sept. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    59017-plasmid-map-sequence-id-423220

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  5. pRVL-6

    Plasmid
    #104584
    Purpose
    Contains human IgG1 CH1/mutated CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Abolished Fc-mediated immune effector functions
    Depositor
    Daniel Christ
    Article
    Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi
    Insert
    Human IgG1 CH1/CH2/CH3 (mutated, no effector functions) (IGHG1 Human)
    Expression
    Mammalian
    Mutation
    L234A/L235E/G237A and K322A/P331S
    Promoter
    CMV
    Available Since
    Jan. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    104584-plasmid-map-sequence-id-198608

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  15. pCRISPReporter-mCherry

    Plasmid
    #65008
    Purpose
    E. coli reporter vector with MCS for inserting bacterial promoter region through 5' end of gene of interest, upstream and in-frame with mCherry reporter.
    Depositor
    Mattheos Koffas
    Article
    Cress et al ACS Synth Biol. 2015 Mar 30.
    Insert
    mCherry
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    User-defined
    Available Since
    May 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    65008-plasmid-map-sequence-id-109895

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  18. 3xFLAG-dCas9/MSCV-EGFP

    Plasmid
    #82613
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Fujita et al Genes Cells. 2018 Feb 26. doi: 10.1111/g
    Insert
    3xFLAG-dCas9
    Use
    Retroviral
    Tags
    3xFLAG tag and NLS (nuclear localization signal)
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available Since
    Sept. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    82613-plasmid-map-sequence-id-298740

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  19. 3xFLAG-dCas9/pMXs-IG

    Plasmid
    #51258
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Hodaka Fujii
    Article
    Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:
    Insert
    3xFLAG-dCas9
    Use
    CRISPR and Retroviral
    Tags
    3xFLAG tag and NLS (nuclear localization signal)
    Expression
    Mammalian
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available Since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only
    51258-plasmid-map-sequence-id-77869

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Showing: 2841 - 2860 of 77447 results