We narrowed to 27,263 results for: ANT

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SS44 pComb3xss anti-SAPAP2 nanobody

Plasmid

#145824

Purpose

Bacterial expression of anti-SAPAP2 (Rat) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-SAPAP2 (Rat) recombinant llama nanobody SS44 (Dlgap2 L. glama (llama))

Tags

6xHis-HA

Expression

Bacterial

Promoter

lac

Available Since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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SS88 pComb3xss anti-SAPAP2 nanobody

Plasmid

#145825

Purpose

Bacterial expression of anti-SAPAP2 (Rat) recombinant llama nanobody for use as an immunolabel

Depositor

James Trimmer

Article

Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

Insert

anti-SAPAP2 (Rat) recombinant llama nanobody SS88 (Dlgap2 L. glama (llama))

Tags

6xHis-HA

Expression

Bacterial

Promoter

lac

Available Since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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pCDF11-SFPQ(214-598)_quadruple_mutant

Plasmid

#135441

Purpose

Expresses SFPQ 214-598 quadruple mutant (L535A, L539A, L546A, M549A) with TEV-cleavable hexahistidine tag

Depositor

Charles Bond

Article

Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

Insert

splicing factor proline and glutamine rich (SFPQ Human)

Tags

Hexahistidine

Expression

Bacterial

Mutation

L535A, L539A, L546A, M549A

Promoter

T7

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-Ataxin-1, 11NQ (Mouse) [N76/8R]

Plasmid

#128641

Purpose

Mammalian Expression Plasmid of anti-Ataxin-1, 11NQ (Mouse). Derived from hybridoma N76/8.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Ataxin-1, 11NQ (Mouse) recombinant mouse monoclonal antibody (Atxn1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-RNF125 Ring Mutant

Plasmid

#122046

Purpose

express RNF125 RM

Depositor

Ze'ev Ronai

Insert

RNF125 RM (RNF125 Human)

Tags

Flag

Expression

Mammalian

Mutation

Ring mutant C37A, C40A

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-PARIS/ZNF746 [N196/16R]

Plasmid

#114475

Purpose

Mammalian Expression Plasmid of anti-PARIS/ZNF746 (Human). Derived from hybridoma N196/16.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-PARIS/ZNF746 (Homo sapiens) recombinant mouse monoclonal antibody (ZNF746 Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-TRIP8b (exon 1a/5) [N291C/22R]

Plasmid

#114563

Purpose

Mammalian Expression Plasmid of anti-TRIP8b (exon 1a/5) (Mouse). Derived from hybridoma N291C/22.

Depositor

James Trimmer

Article

Andrews et al eLife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-TRIP8b (exon 1a/5) (Mus musculus) recombinant mouse monoclonal antibody (Pex5l Mouse)

Expression

Mammalian

Promoter

dual CMV

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-FLAG-WRAP53beta-siRNA-resistant

Plasmid

#64680

Purpose

expresses siRNA resistant WRAP53beta

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

WD40-encoding RNA antisense to P53 (WRAP53 Human)

Tags

FLAG

Expression

Mammalian

Mutation

siRNA resistant

Promoter

CMV

Available Since

May 22, 2015

Availability

Academic Institutions and Nonprofits only
pRSETC-PITPbeta (splice variant 1) K61A

Plasmid

#58271

Purpose

bacterial expression of PITP beta K61A mutant (phosphatidylinositol transfer protein beta)

Depositor

Shamshad Cockcroft

Article

Shadan et al Traffic. 2008 Sep;9(10):1743-56. doi: 10

Insert

PITP beta (PITPNB Human)

Tags

HIS

Expression

Bacterial

Mutation

K61A (abolishes PI binding)

Promoter

T7

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only
Nano-lantern(Ca2+)_63/pRSETB

Plasmid

#51980

Purpose

bacterial expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=63nM

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern_CaM_E104Q-4GS

Tags

6xHis

Expression

Bacterial

Mutation

S257G in Rluc8

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only
TRS6E1b-luc(-732/-721) mutant 4

Plasmid

#45387

Depositor

Thomas Gelehrter

Article

Song et al J Biol Chem. 1998 Nov 6;273(45):29287-90

Insert

6 copies of hPAI-1 promoter -732/-721 four point mutation (SERPINE1 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

mutated from agacaaggttgt to acactaggatga

Available Since

June 20, 2013

Availability

Academic Institutions and Nonprofits only
TRS6E1b-luc(-732/-721) mutant 3

Plasmid

#45386

Depositor

Thomas Gelehrter

Article

Song et al J Biol Chem. 1998 Nov 6;273(45):29287-90

Insert

6 copies of hPAI-1 promoter -732/-721 mutated at -723/-721

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

-723/-721 mutated from tgt to aca

Available Since

June 20, 2013

Availability

Academic Institutions and Nonprofits only
TRS6E1b-luc(-732/-721) mutant 2

Plasmid

#45385

Depositor

Thomas Gelehrter

Article

Song et al J Biol Chem. 1998 Nov 6;273(45):29287-90

Insert

6 copies of hPAI-1 promoter -732/-721 mutated at -730/-728

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Mutation

mutated at -730/-728 from aca to tgt

Available Since

June 20, 2013

Availability

Academic Institutions and Nonprofits only
TRS6E1b-luc(-732/-721) mutant1

Plasmid

#45384

Depositor

Thomas Gelehrter

Article

Song et al J Biol Chem. 1998 Nov 6;273(45):29287-90

Insert

6 copies of hPAI1 promoter -732/-721 mutated at -732/-731

Use

Luciferase

Expression

Mammalian

Mutation

mutated -732/-731 from ag to tc

Available Since

June 20, 2013

Availability

Academic Institutions and Nonprofits only
pM-ErbB4-delta-kinase-PY1 mutant

Plasmid

#17801

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

ErbB4 (ERBB4 Human)

Tags

GAL4-BD

Expression

Mammalian

Mutation

Carboxy-terminal fragment, amino acids 988-1292, …

Available Since

May 9, 2008

Availability

Academic Institutions and Nonprofits only
Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-T4DNApol (JO638)

Plasmid

#208956

Purpose

A variant CE1 construct with T4 DNA polymerase (-exo, activity enhanced), expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

PCV2-XTEN-nSpCas9-BPNLS-T4pol-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

nSpCas9(H840A), T4Pol(-exo;D189A/E191A/L412M)

Promoter

CMV and T7

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-PolBeta (JO574)

Plasmid

#208953

Purpose

A variant CE1 construct with human DNA polymerase beta, expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

PCV2-XTEN-nSpCas9-BPNLS-PolBeta-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

nSpCas9(H840A)

Promoter

CMV and T7

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-TaqStoffel (JO595)

Plasmid

#208952

Purpose

A variant CE1 construct with Taq Stoffel DNA polymerase, expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

PCV2-XTEN-nSpCas9-BPNLS-TaqStoffel-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

nSpCas9(H840A)

Promoter

CMV and T7

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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HUHe variant click editor (CE1) - pCMV-T7-DCV-nCas9-EcKlenow (JO608)

Plasmid

#208946

Purpose

A variant CE1 construct with DCV HUH endonuclease, expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

DCV-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

Promoter

CMV and T7

Available Since

Nov. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pJSC281 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant

Plasmid

#101230

Purpose

Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant C80S/C574S/E60C/D273C/N692A/M694A/Q695A/H698A

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

C80S, C574S, E60C, D273C, N692A, M694A, Q695A and…

Promoter

T7

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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