We narrowed to 5,107 results for: PEP

1. pAAV-hSyn-DIO-EGFP-CAAX

   Plasmid

   #208685

   Purpose

   Expresses the membrane-localized enhanced green fluorescent protein EGFP-CAAX in a cre-dependent manner

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   membrane-localized enhanced green fluorescent protein EGFP-CAAX
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn-GRAB_CRF1.0

   Plasmid

   #208658

   Purpose

   Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0 in neurons

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFAST-BAC BAF155/SMARCC1-His

   Plasmid

   #177863

   Purpose

   Transfer vector to generate recombinant baculovirus expressing BAF155/SMARCC1-His

   Depositor

   Yoshihiro Izumiya

   Article

   Shimoda et al Commun Biol. 2021 Dec 2;4(1):1330. doi:

   Insert

   SMARCC1 (SMARCC1 Human)
   

   Use

   Tags

   His and His tag at C-terminus with GGGGS linker

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   Dec. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pETM33_Nsp15

   Plasmid

   #156481

   Purpose

   Bacterial expression of Sars-CoV2 Nsp15 protein with His-tag and GST-tag

   Depositor

   Ylva Ivarsson

   Article

   Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

   Insert

   Nsp15 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Gene insert is codon optimized for Ecoli.

   Promoter

   T7/LacO

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pETM33_Nsp10

   Plasmid

   #156479

   Purpose

   Bacterial expression of Sars-CoV2 Nsp10 protein with His-tag and GST-tag

   Depositor

   Ylva Ivarsson

   Article

   Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

   Insert

   Nsp10 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Gene insert is codon optimized for Ecoli.

   Promoter

   T7/LacO

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. hV1aR-GFP

   Plasmid

   #67846

   Purpose

   mammalian expression of human V1a receptor with GFP tag

   Depositor

   Christian Gruber

   Article

   Koehbach et al Proc Natl Acad Sci U S A. 2013 Dec 24;11

   Insert

   V1a receptor (AVPR1A Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

7. hV1b-GFP-1

   Plasmid

   #67847

   Purpose

   mammalian expression of human V1b receptor with GFP tag

   Depositor

   Christian Gruber

   Article

   Koehbach et al Proc Natl Acad Sci U S A. 2013 Dec 24;11

   Insert

   V1b receptor (AVPR1B Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pETM33_Nsp4

   Plasmid

   #156474

   Purpose

   Bacterial expression of Sars-CoV2 Nsp4 protein with His-tag and GST-tag

   Depositor

   Ylva Ivarsson

   Article

   Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

   Insert

   Nsp4 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Gene insert is codon optimized for Ecoli.

   Promoter

   T7/LacO

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. WT USP7 TRAF

   Plasmid

   #227226

   Purpose

   Expresses human TRAF-like domain of USP7 with a N-terminal cleavable 6His tag in E. coli

   Depositor

   Irina Bezsonova

   Article

   Valles et al Data Brief. 2020 Dec 23;34:106680. doi:

   Insert

   USP7 (Ubiquitin carboxyl-terminal hydrolase 7) (USP7 Human)
   

   Use

   Tags

   6His

   Expression

   Bacterial

   Mutation

   aa 62-205

   Promoter

   T7-promoter

   Available since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pExp-xMBP-ACE2-CHis

    Plasmid

    #194998

    Purpose

    Production of ACE2 extracellular domain

    Depositor

    Marko Hyvönen

    Article

    Harman et al J Med Chem. 2023 Jul 27;66(14):9881-9893

    Insert

    ACE2 (ACE2 Human)
    

    Use

    Tags

    His8 and xMBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7lac

    Available since

    Jan. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pETM33_Nsp5_Mpro

    Plasmid

    #156475

    Purpose

    Bacterial expression of Sars-CoV2 Nsp5_Mpro protein with His-tag and GST-tag

    Depositor

    Ylva Ivarsson

    Article

    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

    Insert

    Nsp5_Mpro (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA6.2-YFP-CWT

    Plasmid

    #176689

    Purpose

    Fluorescent reporter for phagosomal escape of Staphylococcus aureus

    Depositor

    Martin Fraunholz

    Article

    Blattner et al PLoS Pathog. 2016 Sep 15;12(9):e1005857.

    Insert

    cell wall-targeting domain of Lysostaphin
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    deletion of protase domain of Lysostaphin

    Promoter

    CMV

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-sACE2(WT)-8his

    Plasmid

    #149268

    Purpose

    Mammalian expression plasmid for his-tagged sACE2

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Short GSG linker and 8his tag

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. NLS-4LNDD-pcDNA3.1(+)

    Plasmid

    #172313

    Purpose

    Expresses ADAR2 Deaminase Domain (DD) fused with 4 repeats of lambda N peptide (4LN) with a Nuclear Localization Signal (NLS) in mammalian cells

    Depositor

    Joshua Rosenthal

    Article

    Vallecillo-Viejo et al RNA Biol. 2018 Jan 2;15(1):104-114. doi:

    Insert

    Lambda N peptide - human ADAR2 Deaminase Domain
    

    Use

    Tags

    6xHis, Flag, and Nuclear Localization Signal of S…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMT-sox10-NLS-BirA-2A-mCherry_Ras

    Plasmid

    #80063

    Purpose

    Sox10 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Sox10 promoter

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-DYRK1A(K188R)-2A-mCherry

    Plasmid

    #177166

    Purpose

    Expresses mouse DYRK1A tagged with 2A peptide at it's C-terminus and mCherry in mammalian cells. Lysine at position 188 substituted with Arginine.

    Depositor

    Vance Lemmon

    Article

    Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5

    Insert

    dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1a (Dyrk1a Mouse)
    

    Use

    AAV

    Tags

    2A peptide and mCherry

    Expression

    Mammalian

    Mutation

    Changed Lysine 188 to Arginine

    Promoter

    CMV with beta globin intron

    Available since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3-sACE2(WT)

    Plasmid

    #145147

    Purpose

    Mammalian expression plasmid for soluble ACE2 (protease domain)

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Insert

    Angiotensin-converting enzyme 2 (ACE2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Extracellular, soluble protease domain (a.a. M1-D…

    Promoter

    CMV

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pETM33_Nsp16

    Plasmid

    #156482

    Purpose

    Bacterial expression of Sars-CoV2 Nsp16 protein with His-tag and GST-tag

    Depositor

    Ylva Ivarsson

    Article

    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

    Insert

    Nsp16 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-hSyn-DIO-GRAB_CRF1.0

    Plasmid

    #208661

    Purpose

    Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0 in a cre-dependent manner

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDisplay-DNER

    Plasmid

    #51053

    Purpose

    expression of HA-DNER in mammalian cells

    Depositor

    Mineko Kengaku

    Article

    Eiraku et al J Biol Chem. 2002 Jul 12;277(28):25400-7

    Insert

    DNER (Dner Mouse)
    

    Use

    Tags

    HA and signal peptide

    Expression

    Mammalian

    Mutation

    without endogenous signal peptide (1-221bp)

    Promoter

    CMV

    Available since

    Feb. 19, 2014

    Availability

    Academic Institutions and Nonprofits only