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pTE_16 - pLenti_EFS-Cas9-BLM(1-193)-2A-BlastR

Plasmid

#252012

Purpose

Lentiviral mammalian expression of Cas9-BLM(2-194) TruEditor

Depositor

Neville Sanjana

Article

Burrell et al bioRxiv 2026.02.19.706875

Insert

Cas9-BLM(2-194)

Use

CRISPR and Lentiviral

Mutation

NA

Available Since

March 3, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-N-ALFA-bRho-EGFP

Plasmid

#248792

Purpose

Expresses ALFA-bRho-EGFP in mammalian cells. Modified from Addgene plasmid #45399

Depositor

Michael Robichaux

Article

Hekmatara et al bioRxiv [Preprint]. 2025 Sep 25:2025.09.

Insert

RHO (RHO Bovine)

Tags

ALFA and EGFP

Expression

Mammalian

Available Since

Jan. 22, 2026

Availability

Academic Institutions and Nonprofits only

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pEHE51

Plasmid

#250522

Purpose

Vector for sgRNA expression in Acinetobacter baumannii (CRISPRi). Modified version of pYDE007 (Plasmid #194152) made Golden Gate compatible (BsaI) for sgRNA oligo insertion.

Depositor

Ralph Isberg

Article

Hamami et al bioRxiv [Preprint]. 2025 Aug 4:2024.01.0

Insert

Two BsaI sites (T-BsaI-BfuI-BsaI-A) inserted between the J23119 promoter and dCas9 handle/gRNA scaffold

Expression

Bacterial

Promoter

J23119

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pLenti-Cas9-T2A-mNeonGreen-2-bc6

Plasmid

#250524

Purpose

Expresses Cas9 and mNeonGreen. Barcode is added to the vector.

Depositor

James Eshleman

Article

Teh et al bioRxiv [Preprint]. 2023 Apr 5:2023.04.0

Insert

M13F-AATTGGCC

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 20, 2026

Availability

Academic Institutions and Nonprofits only

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M3-dCAS9-M.SssI (dCas9-M.SssI)

Plasmid

#218782

Purpose

Expresses dCAS9-M.SssI fusion protein and double marker GFP-T2A-Puro under independent promoters

Depositor

Maja Jagodic

Article

Kakhki et al bioRxiv 2025.03.15.641804; doi: 10.1101/

Inserts

M3-dCAS9-M.SssI (dCas9-M.SssI)
GFP-T2A-Puro

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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M3-dCAS9-dDNMT3A (dCas9-d3A)

Plasmid

#218787

Purpose

Expresses dCAS9-dead DNMT3A fusion protein and double marker GFP-T2A-Puro under independent promoters

Depositor

Maja Jagodic

Article

Kakhki et al bioRxiv 2025.03.15.641804; doi: 10.1101/

Inserts

M3-dCAS9-dDNMT3A (dCas9-d3A)
GFP-T2A-Puro

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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M3-dCAS9-DNMT3A

Plasmid

#220237

Purpose

Expresses dCAS9-DNMT3A fusion protein and double marker GFP-T2A-Puro under independent promoters

Depositor

Maja Jagodic

Article

Kakhki et al bioRxiv 2025.03.15.641804; doi: 10.1101/

Inserts

M3-dCAS9-DNMT3A (dCas9-3A)
GFP-T2A-Puro

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 13, 2026

Availability

Academic Institutions and Nonprofits only

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VN1287 pB2Hws_v28_pLWeakRBS_TIR371_cI-Asf1B+rpoA-IP2_Ddcm_WeaKRBS_TIR51_KanR

Plasmid

#235116

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP2.

Depositor

Oscar Ramos

Article

Guyot et al bioRxiv 2025.10.29.680610

Inserts

Asf1B (ASF1B Human)
IP2

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

cI_E34P-rpoA

Expression

Bacterial

Promoter

LtetO and lpp+lacUV5

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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VN1262 pB2Hws_v28_pLWeakRBS_TIR371_cI-Asf1B+rpoA-IP3_Ddcm_WeaKRBS_TIR51_KanR

Plasmid

#235117

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP3.

Depositor

Oscar Ramos

Article

Guyot et al bioRxiv 2025.10.29.680610

Inserts

Asf1B (ASF1B Human)
IP3

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

cI_E34P-rpoA

Expression

Bacterial

Promoter

LtetO and lpp+lacUV5

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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VN550 pAC-cI_E34P-Asf1A_Term_f1-pOL2-62_L-eGFP-KanR

Plasmid

#235102

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses Asf1A.

Depositor

Oscar Ramos

Article

Guyot et al bioRxiv 2025.10.29.680610

Insert

Asf1A (ASF1A Human)

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

cI_E34P

Expression

Bacterial

Promoter

lacUV5

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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VN627 pAC-cI_E34P-Asf1B_Term_f1-pOL2-62_L_eGFP-KanR

Plasmid

#235103

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B.

Depositor

Oscar Ramos

Article

Guyot et al bioRxiv 2025.10.29.680610

Insert

Asf1B (ASF1B Human)

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

cI_E34P

Expression

Bacterial

Promoter

lacUV5

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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VN999 pB2Hws_v9_cI-Asf1B+rpoA-IP3mut3A

Plasmid

#235104

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP3mut3A.

Depositor

Oscar Ramos

Article

Guyot et al bioRxiv 2025.10.29.680610

Inserts

Asf1B (ASF1B Human)
IP3mut3A

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

cI_E34P-rpoA

Expression

Bacterial

Promoter

lacUV5 and lpp+lacUV5

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
VN991 pB2Hws_v9_cI-Asf1B+rpoA-IP1

Plasmid

#235105

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP1.

Depositor

Oscar Ramos

Article

Guyot et al bioRxiv 2025.10.29.680610

Inserts

Asf1B (ASF1B Human)
IP1

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

cI_E34P-rpoA

Expression

Bacterial

Promoter

lacUV5 and lpp+lacUV5

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
VN1043 pB2Hws_v9_cI-Asf1B+rpoA-IP2

Plasmid

#235106

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP2.

Depositor

Oscar Ramos

Article

Guyot et al bioRxiv 2025.10.29.680610

Inserts

Asf1B (ASF1B Human)
IP2

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

cI_E34P-rpoA

Expression

Bacterial

Promoter

lacUV5 and lpp+lacUV5

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
VN990 pB2Hws_v9_cI-Asf1B+rpoA-IP3

Plasmid

#235107

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP3.

Depositor

Oscar Ramos

Article

Guyot et al bioRxiv 2025.10.29.680610

Inserts

Asf1B (ASF1B Human)
IP3

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

cI_E34P-rpoA

Expression

Bacterial

Promoter

lacUV5 and lpp+lacUV5

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
VN1335 pB2Hws_v9_cI-Asf1B+rpoA-IP4

Plasmid

#235108

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP4.

Depositor

Oscar Ramos

Article

Guyot et al bioRxiv 2025.10.29.680610

Inserts

Asf1B (ASF1B Human)
IP4

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

cI_E34P-rpoA

Expression

Bacterial

Promoter

lacUV5 and lpp+lacUV5

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
VN1312 pB2Hws_v30_pLWeakRBS_TIR371_cI-Asf1B+rpoA-IP3mut3A_Ddcm_eGFP-wcKanR

Plasmid

#235109

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP3mut3A.

Depositor

Oscar Ramos

Article

Guyot et al bioRxiv 2025.10.29.680610

Inserts

Asf1B (ASF1B Human)
IP3mut3A

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

cI_E34P-rpoA

Expression

Bacterial

Promoter

LtetO and lpp+lacUV5

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
VN1313 pB2Hws_v30_pLWeakRBS_TIR371_cI-Asf1B+rpoA-IP1_Ddcm_eGFP-wcKanR

Plasmid

#235110

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP1.

Depositor

Oscar Ramos

Article

Guyot et al bioRxiv 2025.10.29.680610

Inserts

Asf1B (ASF1B Human)
IP1

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

cI_E34P-rpoA

Expression

Bacterial

Promoter

LtetO and lpp+lacUV5

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
VN1314 pB2Hws_v30_pLWeakRBS_TIR371_cI-Asf1B+rpoA-IP2_Ddcm_eGFP-wcKanR

Plasmid

#235111

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP2.

Depositor

Oscar Ramos

Article

Guyot et al bioRxiv 2025.10.29.680610

Inserts

Asf1B (ASF1B Human)
IP2

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

cI_E34P-rpoA

Expression

Bacterial

Promoter

LtetO and lpp+lacUV5

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
VN1315 pB2Hws_v30_pLWeakRBS_TIR371_cI-Asf1B+rpoA-IP3_Ddcm_eGFP-wcKanR

Plasmid

#235112

Purpose

Quantitative bacterial two-hybrid (qB2H). Expresses Asf1B and IP3.

Depositor

Oscar Ramos

Article

Guyot et al bioRxiv 2025.10.29.680610

Inserts

Asf1B (ASF1B Human)
IP3

Use

Synthetic Biology; Quantitative bacterial two-hyb…

Tags

cI_E34P-rpoA

Expression

Bacterial

Promoter

LtetO and lpp+lacUV5

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge