We narrowed to 3,760 results for: biorxiv

1. Bxb1-GT_CAG-ITPKA-mScarlet-I

   Plasmid

   #229778

   Purpose

   Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of a mScarlet-I F-actin reporter

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   mScarlet-I
   

   Use

   Synthetic Biology

   Tags

   ITPKA

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. JDW 1391 (pME-V5-FOXF1-P2A-H2A-mCherry)

   Plasmid

   #232132

   Purpose

   A middle entry gateway clone containing V5 tagged human FOXF1 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

   Depositor

   Joshua Wythe

   Article

   Cantu Gutierrez et al bioRxiv 2021.11.15.468651

   Insert

   FOXF1 (FOXF1 Human)
   

   Use

   Gateway cloning

   Tags

   V5

   Expression

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Bxb1-GA_AIO-TetOn_mScarlet_Up-Tandem

   Plasmid

   #229793

   Purpose

   Bxb1-GA donor plasmid with upstream tandem syntax for all-in-one doxycycline-inducible mScarlet expression

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   TRE-mScarlet; rtTA-T2A-mTagBFP2
   

   Use

   Synthetic Biology

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   TRE and CAG

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p35S-FHA-SpAGO4a_gDNA

   Plasmid

   #216841

   Purpose

   N-term Flag-HA tagged Spirodela polyrhiza (Sp9509) AGO4a gDNA under regulation of CaMV 35S promoter

   Depositor

   Arturo Marí-Ordóñez

   Article

   Dombey et al bioRxiv 2024.04.03.587901; doi: https://

   Insert

   ARGONAUTE4
   

   Use

   Tags

   Flag-HA

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S

   Available since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. p35S-FHA-SpAGO4a_cDNA

   Plasmid

   #216842

   Purpose

   N-term Flag-HA tagged Spirodela polyrhiza (Sp9509) AGO4a cDNA under regulation of CaMV 35S promoter

   Depositor

   Arturo Marí-Ordóñez

   Article

   Dombey et al bioRxiv 2024.04.03.587901; doi: https://

   Insert

   ARGONAUTE4
   

   Use

   Tags

   Flag-HA

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S

   Available since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. CLIPf-KIF5A(Δ954-1027)-SNAPf-HisTag-FLAG

   Plasmid

   #200792

   Purpose

   Mammalian expression of CLIPf-KIF5A(Δ954-1027)-SNAPf-HisTag-FLAG

   Depositor

   Alison Twelvetrees

   Article

   Smith et al bioRxiv 2024.12.20.629623

   Insert

   KIF5A (Kif5a Mouse)
   

   Use

   Tags

   CLIPf and SNAPf, HisTag, FLAG

   Expression

   Mammalian

   Mutation

   Δ954-1027

   Promoter

   CMV & T7

   Available since

   Dec. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. CLIPf-KIF5A(411-680)-SNAPf-HisTag-FLAG

   Plasmid

   #200798

   Purpose

   Mammalian expression of CLIPf-KIF5A(411-680)-SNAPf-HisTag-FLAG

   Depositor

   Alison Twelvetrees

   Article

   Smith et al bioRxiv 2024.12.20.629623

   Insert

   KIF5A (Kif5a Mouse)
   

   Use

   Tags

   CLIPf and SNAPf, HisTag, FLAG

   Expression

   Mammalian

   Mutation

   411-680

   Promoter

   CMV & T7

   Available since

   Dec. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEJS2714-CMV-iNme2Smu-ABE8e-i1-L10

   Plasmid

   #228735

   Purpose

   Expresses domain inlaid iNme2Smu nCas9 (D16A)-i1-ABE8e base editor with linker-10 in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   Domain inlaid iNme2Smu nCas9 (D16A)-i1-L10
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Nme2-Cas9, Smu PID, D16A D844G E868K K870R D873A …

   Promoter

   CMV

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEJS2713-CMV-iNme2-ABE8e-i1-L10

   Plasmid

   #228734

   Purpose

   Expresses domain inlaid iNme2 nCas9 (D16A)-i1-ABE8e base editor with linker-10 in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   Domain inlaid iNme2 nCas9 (D16A)-i1-L10
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Nme2-Cas9 D16A D844G E868K K870R D873A D911G K929…

   Promoter

   CMV

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDest-pol2-Smarcd1-Myc

    Plasmid

    #227716

    Purpose

    Expresses mouse Smarcd1 protein with a Myc tag from a Pol2 promoter. Has a Blasticidin resistance gene for stable cell line generation and Ampicilin for plasmid propagation.

    Depositor

    Kent Hunter

    Article

    Ross et al bioRxiv 2024.01.24.577061

    Insert

    Smarcd1 (Smarcd1 Mouse)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    pol2

    Available since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTBL3346 PB-nicking guide-blast

    Plasmid

    #226665

    Purpose

    PiggyBac cargo vector encoding protective nicking sgRNA marked with blasticidin

    Depositor

    Chang Liu

    Article

    Loveless et al bioRxiv 2021.11.05.467507

    Insert

    protective nicking sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTBL435

    Plasmid

    #226628

    Purpose

    pcDNA-dTdT

    Depositor

    Chang Liu

    Article

    Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

    Insert

    catalytically deactivated TdT (DNTT Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    D343E, D345E

    Promoter

    CMV

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLenti-rTetR(SE)-KRAB-2A-mTurquoise

    Plasmid

    #225690

    Purpose

    Lentiviral expression of rTetR(SE) fused to ZNF10 KRAB and mTurquoise-NLS

    Depositor

    Lacra Bintu

    Article

    Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

    Insert

    rTetR-ZNF10 KRAB
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    3xFLAG and T2A-mTurquoise

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBK1302-AAV-EFSNC-dCjCas9-KRAB

    Plasmid

    #223147

    Purpose

    Expression of KRAB with dCjCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    KRAB
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBK1312-AAV-EFSNC-dSaCas9-KRAB

    Plasmid

    #223157

    Purpose

    Expression of KRAB with dSaCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    KRAB
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. HA-MBP-TEVpCS-ADGRG7 CTF-FLAG

    Plasmid

    #217705

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGR7 (ADGRG7 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-415

    Promoter

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPB-cT3G-cERP2-3xFLAG-SMAD1-active

    Plasmid

    #222579

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of 3xFLAG-SMAD1

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    SMAD1 (SMAD1 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    S487E, S489E

    Promoter

    Available since

    Aug. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. His-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)

    Plasmid

    #222990

    Purpose

    Bacterial expression of iGeoCas9(C1) construct

    Depositor

    Jennifer Doudna

    Article

    Chen et al bioRxiv [Preprint]. 2023 Nov 15:2023.11.

    Insert

    His-CL7_MBP_1xNLS_iGeoCas9(C1)_2xNLS(star)
    

    Use

    CRISPR

    Tags

    CL7, His, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGP-CAG-iGABASnFR2(no bind)-WPRE-bGH-polyA

    Plasmid

    #218870

    Purpose

    Mammalian expression of improved GABA sensor control (no change in fluorescence)

    Depositor

    GENIE Project

    Article

    Kolb et al bioRxiv 2025.03.25.644953

    Insert

    iGABASnFR2(no bind)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S99A F102G R168P

    Promoter

    CAG

    Available since

    June 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGP-RSET-iGABASnFR2(no bind)-6xHis

    Plasmid

    #218887

    Purpose

    Bacterial expression of improved GABA sensor control (no change in fluorescence)

    Depositor

    GENIE Project

    Article

    Kolb et al bioRxiv 2025.03.25.644953

    Insert

    iGABASnFR2(no bind)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    S99A F102G R168P

    Promoter

    T7

    Available since

    May 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge