We narrowed to 6,087 results for: nls

1. SpCas9-miniCGBE1

   Plasmid

   #198548

   Purpose

   Expresses human codon-optimized SpCas9-miniCGBE1 and blasticidin resistance: EFS promoter-SpCas9-miniCGBE1-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-miniCGBE1
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. SpCas9-NG-miniCGBE1

   Plasmid

   #198538

   Purpose

   Expresses human codon-optimized SpCas9-NG-miniCGBE1 and blasticidin resistance: EFS promoter-SpCas9-NG-miniCGBE1-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NG-miniCGBE1
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. SpCas9-NRRH

   Plasmid

   #198540

   Purpose

   Expresses human codon-optimized SpCas9-NRRH and blasticidin resistance: EFS promoter-SpCas9-NRRH-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NRRH
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. SpCas9-NRCH

   Plasmid

   #198542

   Purpose

   Expresses human codon-optimized SpCas9-NRCH and blasticidin resistance: EFS promoter-SpCas9-NRCH-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NRCH
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Tol2_-87prox1a:basEGFP_Acry:GFP

   Plasmid

   #218213

   Purpose

   Zebrafish -87prox1a enhancer reporter in the lymphatics at 5 dpf. Acry_GFP as a control in the lens

   Depositor

   Katarzyna Koltowska

   Article

   Panara et al Development. 2024 May 1;151(9):dev202525

   Insert

   -87prox1a
   

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. eA3Ai-max

   Plasmid

   #207166

   Purpose

   Express A3A with an N57G point mutation and an intron in a BE4max-based base editor

   Depositor

   Reuben Harris

   Article

   Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

   Insert

   Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3A (APOBEC3A Human)
   

   Use

   Tags

   Cas9-UGI-UGI-NLS and NLS

   Expression

   Mammalian

   Mutation

   N57G

   Promoter

   CMV

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. AP70_pU.CAG.Cas9-D10A-K848A-R1060A.rBGpA

   Plasmid

   #199254

   Purpose

   Expression construct encoding the high specificity SpCas9-KARA-D10A nickase

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

   Insert

   High specificity S. pyogenes Cas9-D10A-KARA nickase
   

   Use

   CRISPR

   Tags

   3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A K848A R1060A

   Promoter

   CAG promoter

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. AP76_pU.CAG.Cas9-D10A-K848A.rBGpA

   Plasmid

   #199253

   Purpose

   Expression construct encoding the high specificity SpCas9-KA-D10A nickase

   Depositor

   Manuel Goncalves

   Article

   Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

   Insert

   High specificity S. pyogenes Cas9-D10A-KA nickase
   

   Use

   CRISPR

   Tags

   3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A K848A

   Promoter

   CAG promoter

   Available since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAT15543_dABE-SuperFi-Cas9

   Plasmid

   #184373

   Purpose

   Mammalian expression of nuclease inactive SuperFi-Cas9 ABE7 base editor

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

   Insert

   dABE-SuperFi-Cas9
   

   Use

   CRISPR

   Tags

   FLAG, SV40 NLS, and nucleoplasmin NLS

   Expression

   Mammalian

   Mutation

   D10A, H840A, Y1010D, Y1013D, Y1016D, V1018D, R101…

   Promoter

   EF-1α core promoter

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAT15545_dABE8e-SuperFi-Cas9

    Plasmid

    #184375

    Purpose

    Mammalian expression of nuclease inactive SuperFi-Cas9 ABE8e base editor

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

    Insert

    dABE8e-SuperFi-Cas9
    

    Use

    CRISPR

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A, Y1010D, Y1013D, Y1016D, V1018D, R101…

    Promoter

    EF-1α core promoter

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDIV495

    Plasmid

    #177702

    Purpose

    Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hansenii

    Depositor

    Uffe Mortensen

    Article

    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

    Inserts

    Cas9
    Nat
    sgRNA Targeting ADE2 locus in D.hansenii: AGCTAaGCAGATTAAtGCAT
    

    Use

    CRISPR

    Tags

    SV40

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    RNR2p (Debaryomyces hansenii ), SRC1p (Debaryomyc…

    Available since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. deltaVP1 only

    Plasmid

    #166675

    Purpose

    Yeast integrative plasmid for expressing deltaVP1, an NLS deletion mutant of Murine polyomavirus VP1 (GAL1 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

    Insert

    Murine polyomavirus deltaVP1 (NLS deletion mutant)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHA841 - hrp-1p::hrp-1HsLCWT

    Plasmid

    #139198

    Purpose

    Expresses hrp-1p::hrp-1HsLCWT::hrp-1 3'UTR

    Depositor

    Anne Hart

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    hrp-1 (hrp-1 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    last exon replaced with correspoding human sequen…

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHA842 - hrp-1p::hrp-1HsLCD290V

    Plasmid

    #139199

    Purpose

    Expresses hrp-1p::hrp-1HsLCD290V::hrp-1 3'UTR

    Depositor

    Anne Hart

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    hrp-1 (hrp-1 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    D290V, last exon replaced with correspoding human…

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHA847 - mec-4p::hrp-1mScarlet

    Plasmid

    #139201

    Purpose

    Expresses mScarlet tagged hrp-1 in mec-4 neurons

    Depositor

    Anne Hart

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    hrp-1 (hrp-1 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    mScarlet added between RRMs and LC

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHA848 - mec-4p::hrp-1HsLCWTmScarlet

    Plasmid

    #139202

    Purpose

    Expresses mScarlet tagged hrp-1HsLCWT in mec-4 neurons

    Depositor

    Anne Hart

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    hrp-1 (hrp-1 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    mScarlet added between RRMs and LC, last exon rep…

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pHA849 - mec-4p::hrp-1HsLCD290VmScarlet

    Plasmid

    #139203

    Purpose

    Expresses mScarlet tagged hrp-1HsLCD290V in mec-4 neurons

    Depositor

    Anne Hart

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    hrp-1 (hrp-1 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    mScarlet added between RRMs and LC, D290V, last e…

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHA845 - hrp-1p::hrp-1HsLCWTmScarlet

    Plasmid

    #139205

    Purpose

    Expresses mScarlet tagged hrp-1HsLCWT

    Depositor

    Anne Hart

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    hrp-1 (hrp-1 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    mScarlet added between RRMs and LC, last exon rep…

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHA846 - hrp-1p::hrp-1HsLCD290VmScarlet

    Plasmid

    #139206

    Purpose

    Expresses mScarlet tagged hrp-1HsLCD290V

    Depositor

    Anne Hart

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    hrp-1 (hrp-1 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    mScarlet added between RRMs and LC, D290V, last e…

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. hnRNPF_PLD

    Plasmid

    #139113

    Purpose

    express prion like domain of hnRNPF

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al EMBO J. 2021 Feb 1;40(3):e105001. doi: 1

    Insert

    hnRNPF (HNRNPF Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    365-415

    Promoter

    Available since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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