We narrowed to 18,759 results for: nls

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pJT131_GalL_cCDA1-NGBE3

Plasmid

#145095

Purpose

Expressing base editor cCDA1-NGBE3 in yeast cells

Depositor

Ralph Bock

Publication

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

cCDA1-NGBE3

Use

CRISPR

Expression

Yeast

Mutation

spCas9(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1…

Promoter

GalL

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pJT130_GalL_nCDA1-NGBE3

Plasmid

#145094

Purpose

Expressing base editor nCDA1-NGBE3 in yeast cells

Depositor

Ralph Bock

Publication

Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

Insert

nCDA1-NGBE3

Use

CRISPR

Expression

Yeast

Mutation

spCas9(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1…

Promoter

GalL

Available since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pJT79_GalL_nCDA1Δ192-BE3

Plasmid

#145045

Purpose

Expresses nCDA1Δ192-BE3 in yeast cells

Depositor

Ralph Bock

Publication

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

nCDA1Δ192-BE3

Use

CRISPR

Expression

Yeast

Mutation

pmCDA1(1-192AA); spCas9(D10A)

Promoter

GalL

Available since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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P31: FenixS-FUSN-VP16-NLS-IRES-E-Ash1

Plasmid

#250554

Purpose

Mammalian expression of FenixS-FUSN-VP16-NLS and E-Ash1, optogenetic system for far red-light control transcriptional activation

Depositor

Andrew Woolley

Publication

Le et al bioRxiv 2025.11.29.691301

Inserts

FenixS
Ash1

Tags

E protein and FUSN-VP16-NLS

Expression

Mammalian

Promoter

SV40

Available since

Aug. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pSLIK mCh-V5-NES-EGFP-ERBB2 NLS zeo

Plasmid

#192338

Purpose

Lentiviral expression vector for an inducible mCh-V5-NES-EGFP-ERBB2 NLS

Depositor

Kevin Janes

Publication

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

mCh-V5-NES-EGFP-ERBB2 NLS (ERBB2 Human)

Use

Lentiviral

Tags

3xFLAG

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pPB-PGKneo-EF1a-NLS-MARIO-SV40 pA

Plasmid

#247646

Purpose

mammalian expression of NLS-MARIO, a genetically-encoded fluorescent Mg2+ sensor

Depositor

Takeharu Nagai

Publication

Maeshima et al Curr Biol. 2018 Feb 5;28(3):444-451.e6.

Insert

MARIO

Tags

NLS

Expression

Mammalian

Available since

Nov. 24, 2025

Availability

Academic Institutions and Nonprofits only

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RNAPII_CTD27-52

Plasmid

#98678

Purpose

Expresses 6xHis-tagged C-terminal domain of RNA polymerase II

Depositor

Nicolas Fawzi

Publication

Janke et al Biochemistry. 2017 Oct 11. doi: 10.1021/

Insert

RNA polymerase II C-terminal domain (POLR2A Human)

Tags

6xHis

Expression

Bacterial

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEGFP-C1_N-NLS_D652A POLH

Plasmid

#221871

Purpose

Expresses D652A POLH with an N-terminal NLS-GFP tag in mammalian cells

Depositor

Roger Woodgate

Publication

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

NLS-EGFP

Expression

Mammalian

Mutation

D652A

Promoter

CMV

Available since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1_N-NLS_K682A_K686A_K694A_K709A POLH

Plasmid

#221872

Purpose

Expresses K682A_K686A_K694A_K709A POLH with an N-terminal NLS-GFP tag in mammalian cells

Depositor

Roger Woodgate

Publication

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

NLS-EGFP

Expression

Mammalian

Mutation

K682A, K686A, K694A, K709A

Promoter

CMV

Available since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1_N-NLS_L704A_F707A_F708A POLH

Plasmid

#221873

Purpose

Expresses PIP box-mutated POLH with an N-terminal NLS-GFP tag in mammalian cells

Depositor

Roger Woodgate

Publication

Moreno et al Nucleic Acids Res. 2026 Feb 5;54(4):gkag

Insert

POLH (POLH Human)

Tags

NLS-EGFP

Expression

Mammalian

Mutation

L704A_F707A_F708A

Promoter

CMV

Available since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pSmart-EF1alpha-AcrIC8-NLS

Plasmid

#207395

Purpose

Expresses AcrIC8 with NLS in mammalian cells

Depositor

Yan Zhang

Publication

Hu et al Mol Cell. 2024 Feb 1;84(3):463-475.e5. d

Insert

EF1a-AcrIC8-bGH polyA

Tags

NLS

Expression

Mammalian

Promoter

EF1a

Available since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pSmart-EF1alpha-AcrIE2-NLS

Plasmid

#207396

Purpose

Expresses AcrIE2 with NLS in mammalian cells

Depositor

Yan Zhang

Publication

Hu et al Mol Cell. 2024 Feb 1;84(3):463-475.e5. d

Insert

EF1a-AcrIE2-bGH polyA

Tags

NLS

Expression

Mammalian

Promoter

EF1a

Available since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pSmart-EF1alpha-AcrIC9-NLS

Plasmid

#207397

Purpose

Expresses AcrIC9 with NLS in mammalian cells

Depositor

Yan Zhang

Publication

Hu et al Mol Cell. 2024 Feb 1;84(3):463-475.e5. d

Insert

EF1a-AcrIC9-bGH polyA

Tags

NLS

Expression

Mammalian

Promoter

EF1alpha

Available since

Feb. 16, 2024

Availability

Academic Institutions and Nonprofits only

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C-SWAT IAA17-6FLAG (no NLS)

Plasmid

#193322

Purpose

C-SWAT compatible vector for C-terminal tagging with IAA17 (Auxin-Inducible Degron) and 6FLAG. ARF16(PB1)-P2A-osTIR1 has SIR4 homology arms for genome integration and does not have NLS tags..

Depositor

Rob Mitra

Publication

Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

Insert

IAA17

Tags

6FLAG

Expression

Yeast

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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nPPARg429-gs10-NLS-VPR

Plasmid

#188266

Purpose

Plasmid ensures constitutive expression of the N-terminal fragment of split PPARgamma (aa 200-429), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus

Depositor

Roman Jerala

Publication

Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

Insert

nPPARg429 (PPARG Human)

Expression

Mammalian

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pME-QUASM1:GFPNLS-SV40pA

Plasmid

#155116

Purpose

Middle entry vector containing QUASM1 element upstream of GFP-NLS.

Depositor

Bret Pearson

Publication

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QUASM1:GFPNLS

Use

Gateway middle entry vector

Promoter

QUASM1-E1b

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pLPC-Flag-CHES1L-dC-NLS

Plasmid

#51840

Purpose

Flag-CHES1L mutant lacking the C-terminal, with remaining NLS

Depositor

Gerardo Ferbeyre

Publication

Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

Insert

CHES1 (FOXN3 Human)

Use

Retroviral

Tags

Flag and NLS

Mutation

contains CHES1 amino acids 1-205

Available since

July 17, 2014

Availability

Academic Institutions and Nonprofits only
T7-5'UTR-IS621-NLS-3'UTR-polyA (CJT503)

Plasmid

#248221

Purpose

IS621-bpNLS IVT vector for mRNA production

Depositor

Benjamin Kleinstiver

Publication

Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586

Insert

5'UTR-IS621-NLS-3'UTR-polyA

Use

CRISPR; In vitro transcription

Tags

BPNLS

Expression

Mammalian

Mutation

None

Promoter

T7

Available since

May 12, 2026

Availability

Academic Institutions and Nonprofits only

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pDGB3omega2_[EBSn:35Smp:3x SV40-NLS:3x YPet:35Sterm]-[pNOS:KANAMYCIN:tNOS]

Plasmid

#254071

Purpose

3x YPet expression driven by EBSn

Depositor

Jose Alonso, Anna Stepanova

Publication

Fernandez-Moreno et al Plant Biotechnol J. 2026 Feb;24(2):698-7

Insert

EBSn:35Smp:3x SV40-NLS:3x YPet:35Sterm

Use

Synthetic Biology

Expression

Plant

Available since

April 17, 2026

Availability

Academic Institutions and Nonprofits only

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pPL6873_2xcHS4::mCherry::2xcHS4_FRT-PuroR-P2A-mTagBFP2-NLS

Plasmid

#217970

Purpose

Provides expression from a CMV promoter surrounded by insulators. Integrates at FRT site. Confers puromycin resistance. Expresses nuclear localized BFP.

Depositor

Robert Piper

Publication

Minard et al Biol Open. 2025 Oct 15;14(10):bio061793.

Insert

2xcHS4::mCherry::2xcHS4_FRT-PuroR-P2A-mTagBFP2-NLS

Expression

Mammalian

Available since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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