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pmalc4x_V426F_TEV

Plasmid

#47702

Purpose

Expresses MBP myocilin olfactomedin domain fusion protein with a TEV protease recognition sequence and the mutation V426F

Depositor

Raquel Lieberman

Article

Burns et al Biochemistry. 2011 Jul 5;50(26):5824-33.

Insert

Olfactomedin-like domain with V426F mutation (MYOC Human)

Tags

MBP

Expression

Bacterial

Mutation

changed V426F

Promoter

Ptac

Available Since

Sept. 9, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pMuLE ENTR U6-miR-30 L1-L4

Plasmid

#62114

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

RNAi; Mule gateway entry vector

Expression

Mammalian

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR U6-miR-30 L5-L4

Plasmid

#62116

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 promoter and miR30-based hairpin module for shRNA expression. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

RNAi; Mule gateway entry vector

Expression

Mammalian

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only
pMuLE ENTR CMV L3-L2

Plasmid

#62092

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and multiple cloning site module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

Mule gateway entry vector

Expression

Mammalian

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR SV40 L3-L2

Plasmid

#62096

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and multiple cloning site module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

Mule gateway entry vector

Expression

Mammalian

Available Since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pMuLE ENTR CMV R4-R3

Plasmid

#62093

Purpose

MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV promoter and multiple cloning site module. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

Mule gateway entry vector

Expression

Mammalian

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF3B09

Plasmid

#70998

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Hrb87F (Hrb87F Fly)

Use

Gateway entry vector

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only
CMV-huADF WT

Plasmid

#245941

Purpose

Expressing wild type human actin depolymerizing factor (destrin)

Depositor

James Bamburg, Alan Weeds

Article

Hawkins et al Biochemistry. 1993 Sep 28;32(38):9985-93

Insert

actin depolymerizing factor (ADF) (DSTN Human)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pEN_TT PCSK5-V5

Plasmid

#232448

Purpose

Gateway entry vector for an inducible PCSK5

Depositor

Kevin Janes

Article

Marohl et al Mol Cancer Res. 2025 Oct 28. doi: 10.115

Insert

PCSK5 (PCSK5 Human)

Use

Gateway entry vector

Tags

V5

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pEN_TT PCSK5(sgRR)-V5

Plasmid

#232449

Purpose

Gateway entry vector for an inducible sg09 resistant PCSK5

Depositor

Kevin Janes

Article

Marohl et al Mol Cancer Res. 2025 Oct 28. doi: 10.115

Insert

PCSK5 (PCSK5 Human)

Use

Gateway entry vector

Tags

V5

Mutation

a75g_t84c_g87c

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pX458-SLC35A1

Plasmid

#239952

Purpose

Encodes gRNA for human SLC35A1

Depositor

Jan Carette

Article

Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

Insert

SLC35A1

Use

CRISPR

Expression

Mammalian

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pX458-MFSD6

Plasmid

#239951

Purpose

Encodes gRNA for human MFSD6

Depositor

Jan Carette

Article

Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

Insert

MFSD6

Use

CRISPR

Expression

Mammalian

Available Since

July 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-ICAM-5-Puro

Plasmid

#239949

Purpose

CRISPR KO of human ICAM-5

Depositor

Jan Carette

Article

Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

Insert

ICAM-5

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-MFSD6-Puro

Plasmid

#239950

Purpose

CRISPR KO of human MFSD6

Depositor

Jan Carette

Article

Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

Insert

MFSD6

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

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pIDTsmart-RC2-delVP2-VP2-454TAG

Plasmid

#240060

Purpose

Express AAV2 capsid with ncAA mutant at site 454 of VP2

Depositor

Abhishek Chatterjee

Article

Erickson et al Bioconjug Chem. 2024 Jan 17;35(1):64-71.

Inserts

RC2-DelVP2
VP2-454-TAG

Use

AAV

Expression

Mammalian

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pIDTsmart-RC2-delVP1-VP1-454TAG

Plasmid

#240059

Purpose

Express AAV2 capsid with ncAA mutant at site 454 of VP1

Depositor

Abhishek Chatterjee

Article

Erickson et al Bioconjug Chem. 2024 Jan 17;35(1):64-71.

Inserts

RC2-DelVP1
VP1-454-TAG

Use

AAV

Expression

Mammalian

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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IMPT-11371

Plasmid

#236191

Purpose

Express GPR119 in insect cells

Depositor

Vadim Cherezov

Article

Kim et al Structure. 2024 Dec 5;32(12):2206-2219.e

Insert

GPR119 (GPR119 Human)

Expression

Insect

Promoter

polyhedrin

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR2b-PTPN12-Clover

Plasmid

#215521

Purpose

Gateway entry vector with clover-tagged PTP-PEST

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PTPN12 (PTPN12 Human)

Use

Gateway entry vector

Tags

Clover

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR2b-Src(E378G)

Plasmid

#215462

Purpose

Gateway entry vector with Src constitutively-active mutant (E378G)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

Src (SRC Chicken)

Use

Gateway entry vector

Mutation

E378G (constitutively-active)

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR221-VPS35-mScarlet

Plasmid

#215472

Purpose

Gateway entry vector with VPS35 tagged with mScarlet

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

VPS35 (VPS35 Human)

Use

Gateway entry vector

Tags

mScarlet

Promoter

none

Available Since

May 29, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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