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pB-CAG-B19L-IRES-mCherry-WPRE-BGHpA

Plasmid

#178518

Purpose

For making Rabies virus SAD B19 strain L cell line with hyPBase

Depositor

Ian Wickersham

Article

Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

Insert

B19L-IRES-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-EF1a MICA*009-IRES-ZsGreen

Plasmid

#114007

Purpose

Induces expression of human MICA allele 009 cDNA

Depositor

Kai Wucherpfennig

Article

Ferrari de Andrade et al Science. 2018 Mar 30;359(6383):1537-1542

Insert

MICA*009 (MICA Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 8, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-Membrin-IRES-Puromycin

Plasmid

#134865

Purpose

Expresses EGFP-tagged Golgi apparatus marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

Membrin (GOSR2 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 3, 2020

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-EGFP-VAMP2-IRES-Puromycin

Plasmid

#134661

Purpose

Expresses EGFP-tagged synaptic vesicle protein

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

vesicle-associated membrane protein 2 (VAMP2 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pLV UBC APEX2-3xFLAG-IRES-eGFP

Plasmid

#214983

Purpose

Cytosolic APEX2 control. Can be used as a C-terminal APEX2 fusion by digestion with NheI and Gibson cloning (see comments).

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

APEX2 (APX2 Glycine max (soybean))

Use

Lentiviral

Tags

3xFLAG

Expression

Mutation

Promoter

UBC

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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MSCV-EZH2-PGK-Puro-IRES-GFP

Plasmid

#75125

Purpose

Retroviral expression plasmid encoding wild type human EZH2

Depositor

Christopher Vakoc

Article

Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

Insert

KMT6 (EZH2 Human)

Use

Retroviral

Tags

None

Expression

Mutation

Promoter

Available since

May 20, 2016

Availability

Academic Institutions and Nonprofits only
pMXS-IRES-BLAST flag-CAD S1859A

Plasmid

#169821

Purpose

Expresses C-terminal flag-tagged CAD with mutation of reported S6K phosphorylation site

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

S1859A; TCCC -> AGTC silent mutations at nt527…

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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p3E-IRES-3xNLS-mScarlet-I3-pA (JDW 1360)

Plasmid

#224536

Purpose

A Gateway compatible 3' entry clone containing IRES-FLAG- NLS-mScarlet-I3

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

IRES-FLAG- NLS-mScarlet-I3

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-CDK8-W105M-IRES-mCherry

Plasmid

#68857

Purpose

Expresses Human CDK8 W105M Mutant

Depositor

Matthew Shair

Article

Pelish et al Nature. 2015 Sep 28. doi: 10.1038/nature

Insert

Cyclin-Dependent Kinase 8 (CDK8 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

W105M

Promoter

EF1α

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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CAG-DDdCas9VP192-T2A-EGFP-ires-puro

Plasmid

#69534

Purpose

DHFR destabilised domain (DD) fused to dCas9VP192 (S.pyogenes) on CAG expression vector. DDdCas9VP192 protein is stabilised by Trimethoprim.

Depositor

Timo Otonkoski

Article

Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

Insert

DD-dCas9VP192-T2A-EGFP

Use

Tags

C-term T2A-EGFP and DHFR Destabilised Domain

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only

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