We narrowed to 5,601 results for: Feng Zhang

1. pBoBi-hPEN2 D90A

   Plasmid

   #183641

   Purpose

   Lentiviral expression of a PEN2 mutant losing its binding affinity to metformin at the C-terminus (lysosome lumen).

   Depositor

   Sheng-cai Lin

   Article

   Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

   Insert

   PEN2 (PSENEN Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   residues 90 of PEN2 mutated to alanine

   Promoter

   CMV

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBoBi-hPEN2 20A

   Plasmid

   #183639

   Purpose

   Lentiviral expression of a PEN2 mutant showing impaired affinity with ATP6AP1.

   Depositor

   Sheng-cai Lin

   Article

   Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

   Insert

   PEN2 (PSENEN Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   residues 27, 28, 30, 31, 34, 38, 42, 43, 57, 58, …

   Promoter

   CMV

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBoBi-hSEC61B-C-PEN2

   Plasmid

   #183648

   Purpose

   Lentiviral expression of a ER-residential PEN2.

   Depositor

   Sheng-cai Lin

   Article

   Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

   Insert

   PEN2 and SEC61B (PSENEN, SEC61B Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   WT

   Promoter

   CMV

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBoBi-hPEN2 2A

   Plasmid

   #183640

   Purpose

   Lentiviral expression of a PEN2 mutant losing its binding affinity to metformin at the N-terminus (cytosolic face).

   Depositor

   Sheng-cai Lin

   Article

   Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

   Insert

   PEN2 (PSENEN Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   residues 35 and 40 of PEN2 mutated to alanine

   Promoter

   CMV

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. mCherry-Cytb5

   Plasmid

   #182579

   Purpose

   Expresses mCherry targeted to the ER via the CytB5RR tail anchor sequence

   Depositor

   Uri Manor

   Article

   Schiavon et al Nat Methods. 2020 Sep;17(9):917-921. doi

   Insert

   mCherry-Cytb5
   

   Use

   Tags

   Cytb5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV-ABE

   Plasmid

   #107723

   Purpose

   For expression of ABE7.10 in mammalian cells

   Depositor

   Xingxu Huang

   Article

   Liu et al Nat Commun. 2018 Jun 14;9(1):2338. doi:

   Insert

   ABE7.10
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. AXBEv2

   Plasmid

   #204606

   Purpose

   Mammalian expression of evolved adenine transversion base editor AXBEv2

   Depositor

   Dali Li

   Article

   Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103

   Insert

   bpNLS-TadA-8e-nSpCas9-mAAG (R165E/Y179F)-bpNLS-P2A-GFP
   

   Use

   CRISPR

   Tags

   6*His

   Expression

   Mammalian

   Mutation

   mAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)

   Promoter

   Available since

   Aug. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. ACBE

   Plasmid

   #204607

   Purpose

   Mammalian expression of adenine-to-cytosine base editor ACBE

   Depositor

   Dali Li

   Article

   Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103

   Insert

   bpNLS-N terminal of nSpCas9-linker-mAAG (R165E/Y179F)-TadA-8e-linker-C terminal of nSpCas9-bpNLS-P2A-GFP
   

   Use

   CRISPR

   Tags

   6*His

   Expression

   Mammalian

   Mutation

   mAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)

   Promoter

   Available since

   Aug. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. AXBE

   Plasmid

   #204605

   Purpose

   Mammalian expression of adenine transversion base editor AXBE

   Depositor

   Dali Li

   Article

   Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103

   Insert

   bpNLS-TadA-8e-nSpCas9-mAAG-bpNLS-P2A-GFP
   

   Use

   CRISPR

   Tags

   6*His

   Expression

   Mammalian

   Mutation

   S. pyogenes Cas9 (D10A)

   Promoter

   Available since

   Aug. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBoBi-hLAMP2-C-PEN2

    Plasmid

    #183646

    Purpose

    Lentiviral expression of a lysosome-residential PEN2.

    Depositor

    Sheng-cai Lin

    Article

    Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

    Insert

    PEN2 and LAMP2 (PSENEN, LAMP2 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    WT

    Promoter

    CMV

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3.3-hAP1 1-419

    Plasmid

    #183644

    Purpose

    To (ectopically) express a mutant of ATP6AP1 lacking the interacting domain with PEN2; mammalian expression

    Depositor

    Sheng-cai Lin

    Article

    Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

    Insert

    ATP6AP1 (ATP6AP1 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    truncated ATP6AP1 mutant; contains only amino aci…

    Promoter

    CMV

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3.3-hAP1 420-440

    Plasmid

    #183643

    Purpose

    To (ectopically) express a mutant of ATP6AP1 lacking the interacting domain with PEN2; mammalian expression

    Depositor

    Sheng-cai Lin

    Article

    Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

    Insert

    ATP6AP1 (ATP6AP1 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    truncated ATP6AP1 mutant; contains only the trans…

    Promoter

    CMV

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBoBi-hAP1 Δ420-440

    Plasmid

    #183642

    Purpose

    To (stably) express a mutant of ATP6AP1 lacking the interacting domain with PEN2; lentiviral expression

    Depositor

    Sheng-cai Lin

    Article

    Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

    Insert

    ATP6AP1 (ATP6AP1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    truncated ATP6AP1 mutant lacking the transmembran…

    Promoter

    CMV

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLentiCRISPR_mCherry

    Plasmid

    #229078

    Purpose

    Small lentiviral backbone for CRISPR guide libraries, improved titre for hard to transduce cells

    Depositor

    Bernhard Schmierer

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1s

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PH-Btk-mRuby2

    Plasmid

    #124657

    Purpose

    PI(3,4,5)P3 probe. PH domain of BTK fused with mRuby2

    Depositor

    Cheng-han Yu

    Article

    Zhang et al Mol Biol Cell. 2019 Mar 1;30(5):622-635.

    Insert

    BTK (BTK Human)
    

    Use

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    contains only the PH domain of Bruton tyrosine ki…

    Promoter

    Available since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAdEasy-hALDOA

    Plasmid

    #210812

    Purpose

    To package adenovirus expressing wild-type aldolaseA

    Depositor

    Sheng-cai Lin

    Article

    Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

    Insert

    ALDOA (ALDOA Human)
    

    Use

    Adenoviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAdEasy-hALDOA K230A

    Plasmid

    #210814

    Purpose

    To block the formation of the initial Schiff base with FBP

    Depositor

    Sheng-cai Lin

    Article

    Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

    Insert

    ALDOA (ALDOA Human)
    

    Use

    Adenoviral

    Tags

    HA

    Expression

    Mutation

    residues 230 of ALDOA mutated to alanine

    Promoter

    CMV

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.3-hALDOA K230A

    Plasmid

    #210811

    Purpose

    To block the formation of the initial Schiff base with FBP

    Depositor

    Sheng-cai Lin

    Article

    Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

    Insert

    ALDOA (ALDOA Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    residues 230 of ALDOA mutated to alanine

    Promoter

    CMV

    Available since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBoBi-hALDOA K230A

    Plasmid

    #210808

    Purpose

    To block the formation of the initial Schiff base with FBP

    Depositor

    Sheng-cai Lin

    Article

    Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

    Insert

    ALDOA (ALDOA Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    residues 230 of ALDOA mutated to alanine

    Promoter

    CMV

    Available since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pT22UbGfpnE4PCh(#301)

    Plasmid

    #184066

    Purpose

    cyclofen-inducible GFP nuclear relocation in zebrafish permanent transgenic

    Depositor

    Sophie Vriz

    Article

    Zhang et al Chembiochem. 2018 Jun 18;19(12):1232-123

    Insert

    eGFP-nls-ERT2-P2A-mCherry
    

    Use

    Zebrafish transgenesis (blue eyes)

    Tags

    Expression

    Mutation

    ERT2: G400V, M543A, L544A, deleted 1-281;

    Promoter

    Zf-Ubi

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGL3-U6-sgRNA-EGFP

    Plasmid

    #107721

    Purpose

    For expression of sgRNA from the U6 promoter with EGFP expression simultaneously

    Depositor

    Xingxu Huang

    Article

    Liu et al Nat Commun. 2018 Jun 14;9(1):2338. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. LifeAct-GFP-Fis1

    Plasmid

    #182576

    Purpose

    Expresses LifeAct-GFP targeted to the outer mitochondrial membrane via the Fis1 tail anchor sequence

    Depositor

    Uri Manor

    Article

    Schiavon et al Nat Methods. 2020 Sep;17(9):917-921. doi

    Insert

    LifeAct-GFP-Fis1
    

    Use

    Tags

    Fis1 and LifeAct

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. mCherry-Fis1

    Plasmid

    #182580

    Purpose

    Expresses mCherry targeted to the outer mitochondrial membrane via the Fis1 tail anchor sequence

    Depositor

    Uri Manor

    Article

    Schiavon et al Nat Methods. 2020 Sep;17(9):917-921. doi

    Insert

    mCherry-Fis1
    

    Use

    Tags

    Fis1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. LifeAct-GFP-cytoB5RR

    Plasmid

    #182577

    Purpose

    Expresses LifeAct-GFP targeted to the ER via the CytB5RR tail anchor sequence

    Depositor

    Uri Manor

    Article

    Schiavon et al Nat Methods. 2020 Sep;17(9):917-921. doi

    Insert

    LifeAct-GFP-cytoB5RR
    

    Use

    Tags

    LifeAct and cytoB5RR

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGL3-U6-sgRNA-BFP

    Plasmid

    #107722

    Purpose

    For expression of sgRNA from the U6 promoter with BFP expression simultaneously

    Depositor

    Xingxu Huang

    Article

    Liu et al Nat Commun. 2018 Jun 14;9(1):2338. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBS human IRS-1

    Plasmid

    #11359

    Depositor

    Ronald Kahn

    Article

    Araki et al Diabetes. 1993 Jul . 42(7):1041-54.

    Insert

    IRS-1 (IRS1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 1, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pUC57-Sa sgRNA expression vector

    Plasmid

    #107720

    Purpose

    For in vitro transcription of Sa sgRNA from the T7 promoter.

    Depositor

    Xingxu Huang

    Article

    Liu et al Nat Commun. 2018 Jun 14;9(1):2338. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pT7-AGG-NLS-EGFP

    Plasmid

    #214602

    Purpose

    express EGFP ; in vitro transcribed modified RNA vector

    Depositor

    Yuxuan Guo

    Article

    Huang et al Int Immunopharmacol. 2024 Oct 17;143(Pt

    Insert

    EGFP
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pT7-AGG-SpCas9

    Plasmid

    #214603

    Purpose

    encode SpCas9; in vitro transcribed modified RNA vector

    Depositor

    Yuxuan Guo

    Article

    Huang et al Int Immunopharmacol. 2024 Oct 17;143(Pt

    Insert

    SpCas9
    

    Use

    CRISPR and Unspecified

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBoBi-hAP1-C-LAMP2 TM

    Plasmid

    #183645

    Purpose

    Lentiviral expression of a mutant of ATP6AP1 with its PEN2-interacting domain replaced with the transmembrane (TM) domain of LAMP2.

    Depositor

    Sheng-cai Lin

    Article

    Ma et al Nature. 2022 Mar;603(7899):159-165. doi:

    Insert

    ATP6AP1 and LAMP2 (ATP6AP1, LAMP2 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    ATP6AP1 transmembrane domain replaced by the tran…

    Promoter

    CMV

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge