We narrowed to 18,386 results for: Met

1. mEos4a-N1

   Plasmid

   #54811

   Purpose

   Localization: N1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581

   Depositor

   Loren Looger, Michael Davidson

   Article

   Paez-Segala et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

   Type

   Empty backbone

   Tags

   mEos4a

   Expression

   Mammalian

   Available Since

   June 20, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. M-NMn-VP64

   Plasmid

   #48676

   Purpose

   Mammalian NM-VP64 nuclease-null Cas9 activator expression, human optimized

   Depositor

   George Church

   Article

   Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

   Insert

   Cas9-VP64, nuclease-null
   

   Use

   CRISPR

   Tags

   NLS and VP64

   Mutation

   nuclease-null

   Promoter

   CMV

   Available Since

   Oct. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCFJ910

   Plasmid

   #44481

   Depositor

   Erik Jorgensen

   Article

   Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

   Insert

   Minimal Mos1, NeoR, MCS
   

   Use

   Multiple cloning site vector

   Available Since

   July 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CMV-Cas9C-VPR

   Plasmid

   #80933

   Purpose

   Expresses Cas9C-VPR in mammalian cells.

   Depositor

   George Church

   Article

   Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

   Insert

   Cas9C-VPR
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRV∆L-5Cre

   Plasmid

   #182964

   Purpose

   The 3rd generation of rabies vector expressing Cre recombinase

   Depositor

   Ian Wickersham

   Article

   Jin et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   CRE recombinase
   

   Use

   Rabies

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-SMVP-Cas9N-U6-gRNA M3

   Plasmid

   #80936

   Purpose

   Expresses Cas9N in mammalian cells; expresses gRNA M3 for Mstn cleavage.

   Depositor

   George Church

   Article

   Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

   Insert

   Cas9N
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. Ii-Str_ManII-SBP-EGFP

   Plasmid

   #65255

   Purpose

   synchronize trafficking of ManII from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Ii-Streptavidin and truncated Mannosidase II
   

   Tags

   EGFP and Streptavidin Binding Protein (SBP)

   Expression

   Mammalian

   Mutation

   contains only amino acids 1 to 116 of ManII

   Promoter

   CMV

   Available Since

   June 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. Ii-Str_ManII-SBP-mCherry

   Plasmid

   #65256

   Purpose

   synchronize trafficking of ManII from the ER

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Ii-Streptavidin and truncated Mannosidase II
   

   Tags

   Streptavidin Binding Protein (SBP) and mCherry

   Expression

   Mammalian

   Mutation

   contains only amino acids 1 to 116 of ManII

   Promoter

   CMV

   Available Since

   June 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-AKAR2-CR

   Plasmid

   #40255

   Depositor

   Michael Lin

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Insert

   AKAR2
   

   Tags

   His6 and Xpress

   Expression

   Mammalian

   Promoter

   CMV IE

   Available Since

   Oct. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pmScarlet-I_peroxisome_C1

    Plasmid

    #85065

    Purpose

    In vivo visualization of the peroxisome (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    Peroxisomal targeting sequence
    

    Tags

    mScarlet-i

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTriEx-mCherry-Zdk1-TIAM1 DH/PH

    Plasmid

    #81129

    Purpose

    mammalian expression of Zdk1 tagged Tiam1 Dh/PH

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Inserts

    Zdk1
    TIAM1 DH/PH (TIAM1 Human)
    

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB-CT3G-ERP2-MG

    Plasmid

    #175501

    Purpose

    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements (hEF1a-driven rtTA and puromycin resistance)

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    TRE3G-mCMV

    Available Since

    Oct. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDH_SBP-EGFP-Ecadherin

    Plasmid

    #65290

    Purpose

    Ecadherin reporter only (no hook) (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Ecadherin
    

    Use

    Lentiviral

    Tags

    EGFP and IL-2 Signal Sequence and Streptavidin Bi…

    Promoter

    CMV

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTRIPZ-NLS-PTEN

    Plasmid

    #206984

    Purpose

    Lentiviral vector for expressing doxycyclin-inducible cDNA of PTEN with NLS

    Depositor

    Ming Chen

    Article

    Loh et al Metabolites. 2023 Aug 11;13(8):939. doi:

    Insert

    PTEN (PTEN Human)
    

    Use

    Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    tet-on promoter

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmScarlet-H_NES_C1

    Plasmid

    #85061

    Purpose

    In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    NES
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLncEXP

    Plasmid

    #64865

    Purpose

    Empty vector for lncRNA expression

    Depositor

    Lei Sun

    Article

    Alvarez-Dominguez et al Cell Metab. 2015 May 5;21(5):764-76. doi

    Type

    Empty backbone

    Use

    Retroviral

    Promoter

    CMV

    Available Since

    May 26, 2015

    Availability

    Academic Institutions and Nonprofits only

17. AB.pCCL.sin.cPPT.GFP.miR-126-3p.sensor.PGK.dNGFR.WPRE

    Plasmid

    #85908

    Purpose

    Sensor Plasmid

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-126-3p Sensor (MIR126 Human)
    

    Use

    Lentiviral

    Promoter

    PGK

    Available Since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAct:dCas9-GCN4

    Plasmid

    #78899

    Purpose

    Expresses dCas9-GCN4 under actin promoter for SunTag CRISPRa in Drosophila cell culture

    Depositor

    Norbert Perrimon

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    dCas9-10XGCN4
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Insect

    Promoter

    pActin (Drosophila)

    Available Since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEF_dCas9-VP64

    Plasmid

    #68417

    Purpose

    Transient expression of Sp-dCas9 fused to the VP64 transcription activator, in mammalian cells, under an EF1-alpha promoter.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and VP64

    Expression

    Mammalian

    Mutation

    D10A/H841A

    Promoter

    human EF1[alpha]

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCFJ909

    Plasmid

    #44480

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

    Insert

    Minimal Mos1, unc-119, MCS
    

    Use

    Multiple cloning site vector

    Available Since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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