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R701-X57-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-169) Q61L

Plasmid

#159565

Purpose

E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-169) Q61L

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-Avi-Hs.KRAS4b(2-169) Q61L (KRAS Human)

Tags

His6-MBP-tev-Avi

Expression

Bacterial

Mutation

Q61L

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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R701-X19-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G12V

Plasmid

#159570

Purpose

E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G12V

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G12V (KRAS Human)

Tags

His6-MBP-tev-Avi

Expression

Bacterial

Mutation

G12V

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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R701-X20-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G12C

Plasmid

#159568

Purpose

E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G12C

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-Avi-Hs.KRAS4b(2-188) G12C (KRAS Human)

Tags

His6-MBP-tev-Avi

Expression

Bacterial

Mutation

G12C

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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R701-X24-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-188) Q61R

Plasmid

#159573

Purpose

E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-188) Q61R

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-Avi-Hs.KRAS4b(2-188) Q61R (KRAS Human)

Tags

His6-MBP-tev-Avi

Expression

Bacterial

Mutation

Q61R

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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R701-X23-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-188) Q61L

Plasmid

#159572

Purpose

E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-188) Q61L

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-Avi-Hs.KRAS4b(2-188) Q61L (KRAS Human)

Tags

His6-MBP-tev-Avi

Expression

Bacterial

Mutation

Q61L

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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R701-X58-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-169) Q61R

Plasmid

#159566

Purpose

E. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-169) Q61R

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-Avi-Hs.KRAS4b(2-169) Q61R (KRAS Human)

Tags

His6-MBP-tev-Avi

Expression

Bacterial

Mutation

Q61R

Available Since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-355 C-Tag Venus173/155[2] L10 {Esp3I(2x)} mCherry

Plasmid

#129858

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Venus

Expression

Mammalian

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-356 N-Tag Venus173/155[2] L10 {Esp3I(2x)} mCherry

Plasmid

#129859

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Venus

Expression

Mammalian

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-357 C-Tag Venus173/155[2] L20 {Esp3I(2x)} mCherry

Plasmid

#129860

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Venus

Expression

Mammalian

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-358 N-Tag Venus173/155[2] L20 {Esp3I(2x)} mCherry

Plasmid

#129861

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Venus

Expression

Mammalian

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-360 N-Tag Venus173/155[2] L32 {Esp3I(2x)} mCherry

Plasmid

#129863

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Venus

Expression

Mammalian

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-359 C-Tag Venus173/155[2] L32 {Esp3I(2x)} mCherry

Plasmid

#129862

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Venus

Expression

Mammalian

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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NSK266 SFFV-mCherry-SGc(EBER1.linear.2)cSG-EGFP-(3xMS2) (FLP-IN)

Plasmid

#247287

Purpose

Linear sensor for EBER1

Depositor

Xiaojing Gao

Article

Kolber et al bioRxiv 2025.09.18.677213

Insert

mCherry-os13.EBER1.linear.2-EGFP

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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Thy-1-CRISPR-gRNA#2-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

Plasmid

#124284

Purpose

sgRNA against human Thy-1

Depositor

Katalin Josvay, Csaba Vizler

Insert

Homo sapiens Thy-1 cell surface antigen (THY1), transcript variant 1 (THY1 Human)

Use

CRISPR

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only
3xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2 probasin_mTQ2_FlpO

Plasmid

#68405

Purpose

The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex8 and SMAD4 ex2. are expressed by the U6 promoter

Depositor

Jannik Elverløv-Jakobsen

Inserts

3xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2
FlpO

Use

CRISPR

Tags

mTurquoise2 (BFP)

Expression

Mammalian

Promoter

Synthetic Probasin ARRx2 and U6

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only

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EW415 PGK-ZNF35(5-8)-IKZF1(2-3)-realDeimmunLink-NZF (FLP-IN)

Plasmid

#236136

Purpose

Plasmid encoding the ZNF35/ZNFIKZF1 zinc finger array attached by a deimmunized linker to the NZF transcriptional activation domain, under control of PGK promoter

Depositor

Xiaojing Gao

Article

Wolfsberg et al bioRxiv 2025.02.24.639918

Insert

ZNF35/IKZF1(2-3)-deimmunLink-NZF

Expression

Mammalian

Promoter

PGK

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pSLQ9834_sgBB: pHR-hU6-CasMINI sgRNA_#2; EF1a-Puro-T2A-BFP- WPRE

Plasmid

#180280

Purpose

The CasMINI sgRNA cloning backbone with sites 'BsmBI' for inserting new guide sequences.

Depositor

Stanley Qi

Article

Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

Insert

CasMINI sgRNA (backbone) and BFP

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Tornado-F30-Broccoli-Squash-SAM-sensor 4-2

Plasmid

#177919

Purpose

Expresses ratiometric Squash-SAM sensor 4-2 as circular RNA in mammalian cells using the Tornado expression system.

Depositor

Samie Jaffrey

Article

Dey et al Nat Chem Biol. 2021 Dec 22. pii: 10.1038

Insert

Tornado-F30-Broccoli-Squash-SAM-sensor 4-2

Expression

Mammalian

Promoter

U6+27

Available Since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCFJ2440 - [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), no_atg, no stop)

Plasmid

#159849

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), no_atg, no stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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-962 human cyclin D1 promoter TCF (1)(2) sites mutant pGL3Basic

Plasmid

#32734

Depositor

Frank McCormick

Article

Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

Insert

CCND1 promoter (CCND1 Human)

Use

Luciferase

Mutation

-75 TCF(1) and -68 TCF(2) - CTTTGATCTTTGCT deleti…

Promoter

-962 CCND1

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only