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pMJ529: pU6-SpuFz1_MS2-omegaRNA(75nt)-Guide(B2M)

Plasmid

#205278

Purpose

Human expression vector for SpuFz1 omegaRNA 75-nt scaffold + 5' MS2- (Guide:B2M)

Depositor

Feng Zhang

Publication

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1 omegaRNA

Expression

Mammalian

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pME-PLIN2-tdTOMATO

Plasmid

#172533

Purpose

PLIN2-tdTOMATO fusion (zebrafish lipid droplet reporter) in middle entry vector for Gateway cloning

Depositor

Richard White

Publication

Lumaquin et al Elife. 2021 Jun 11;10. pii: 64744. doi:

Insert

plin2-tdtomato

Mutation

S80R, A81S, E144D, I148F, and R415H mutations in …

Available since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pTM-BPL

Plasmid

#173766

Purpose

Fluorescent labeling of the nuclear envelope

Depositor

Shinji Sueda

Publication

Taniyama et al ACS Chem Biol. 2018 Jun 15;13(6):1463-14

Insert

PDGF receptor TM domain, Biotin protein ligase

Tags

FLAG and Signal peptide

Expression

Mammalian

Promoter

CMV

Available since

Sept. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pBCCP-GFP-NLS

Plasmid

#173923

Purpose

Fluorescent labeling of the nuclear envelope

Depositor

Shinji Sueda

Publication

Taniyama et al ACS Chem Biol. 2018 Jun 15;13(6):1463-14

Insert

Biotin carboxyl carrier protein, AcGFP1

Tags

3xNLS

Expression

Mammalian

Promoter

CMV

Available since

Sept. 9, 2021

Availability

Academic Institutions and Nonprofits only

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EPS15-GFP-His

Plasmid

#170860

Purpose

Mammalian expression of EPS15-GFP-His.

Depositor

Justin Taraska

Publication

Prasai et al Nat Commun. 2021 Jun 25;12(1):3970. doi:

Insert

Epidermal growth factor receptor pathway substrate 15 (EPS15) (EPS15 Human)

Tags

(His)6 and EGFP

Expression

Mammalian

Promoter

CMV

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA5-FRT-TetO-U1-70K-fSNAPc

Plasmid

#106987

Purpose

open reading frame of U1-70K inserted into pcDNA5-FRT-TetO-fSNAPc using the KpnI and NotI restriction sites

Depositor

Melissa Moore

Publication

Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

Insert

U1-70K (SNRNP70 Human)

Tags

fSNAP

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pX330A_D10A-1x6

Plasmid

#58776

Purpose

Expresses Cas9 nickase and gRNA

Depositor

Takashi Yamamoto

Publication

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pFastBac1 PIK3R1-Ni

Plasmid

#17744

Depositor

Bert Vogelstein

Publication

Samuels et al Cancer Cell. 2005 Jun . 7(6):561-73.

Insert

PIK3R1-Ni (PIK3R1 Human)

Expression

Insect

Mutation

Partial cDNA

Available since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

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pDisplay FLIPE-1m

Plasmid

#13548

Depositor

Wolf Frommer

Publication

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E A207W

Tags

ECFP and Venus

Expression

Mammalian

Mutation

A207W in binding protein

Available since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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Jnk3a1

Plasmid

#29577

Depositor

Dustin Maly

Publication

Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

Insert

Jnk3a1 (MAPK10 Human)

Tags

HIS6 and TEV

Expression

Bacterial

Mutation

Contains residues 40-402

Available since

July 16, 2011

Availability

Academic Institutions and Nonprofits only
TLS 3: TLS.pRSET.C

Plasmid

#21829

Depositor

David Ron

Publication

Crozat et al Nature. 1993 Jun 17;363(6430):640-4.

Insert

TLS (FUS Human)

Tags

6xHis

Expression

Bacterial

Available since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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TCRalpha-L1

Plasmid

#11262

Depositor

Lisa Steiner

Publication

Danilova et al Dev Comp Immunol. 2004 Jun . 28(7-8):755

Insert

TCR alpha (trac Zebrafish)

Expression

Bacterial

Available since

March 15, 2006

Availability

Academic Institutions and Nonprofits only
pRSF-KpAGO207-1251

Plasmid

#117854

Purpose

Expressed KpAGO 207-1251 in E. coli under IPTG-inducible promotor

Depositor

Kotaro Nakanishi, Dinshaw Patel

Publication

Nakanishi et al Nature. 2012 Jun 20;486(7403):368-74. do

Insert

Argonaute

Tags

6xHis-SUMO encoded in vector

Expression

Bacterial

Mutation

missing first 206 amino acid residues

Promoter

T7

Available since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pOX + kqt1

Plasmid

#16136

Depositor

Larry Salkoff

Publication

Wei et al J Biol Chem. 2005 Jun 3. 280(22):21337-4

Insert

Caenorhabditis elegans potassium channel (kqt-1 Nematode)

Use

Xenopus oocyte expression

Mutation

N/A

Available since

Jan. 10, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
Syn3E34A

Plasmid

#13401

Depositor

Thomas Weimbs

Publication

Sharma et al J Cell Biol. 2006 Jun 19. 173(6):937-48.

Insert

Syntaxin3 (STX3 Human)

Tags

Myc-His

Expression

Mammalian

Mutation

Insert is cloned into a modified vector. E34 of s…

Available since

Nov. 9, 2006

Availability

Academic Institutions and Nonprofits only
pRATIO2151

Plasmid

#141300

Purpose

Gateway cloning compatible ratiometric binary vector

Depositor

David Nelson

Publication

Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

Type

Empty backbone

Tags

Firefly Luciferase or LUC2 and mScarlet-I

Expression

Plant

Promoter

UBQ10 promoter

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pRS416-PHO5-GFP-Osh3

Plasmid

#58839

Purpose

Expresses GFP-Tagged Osh3 in yeast

Depositor

Tim Levine, Sean Munro

Publication

Levine et al Mol Biol Cell. 2001 Jun;12(6):1633-44.

Insert

Osh3p (OSH3 Budding Yeast)

Tags

GFP and Myc

Expression

Yeast

Promoter

PHO5

Available since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pCAG-HASAP

Plasmid

#138325

Purpose

chemigenetic voltage indicator

Depositor

Eric Schreiter

Publication

Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

Insert

HASAP

Expression

Mammalian

Promoter

CAG

Available since

March 10, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHAGE2-EF1aL-mCherry-W

Plasmid

#126703

Purpose

Constitutive, ubiquitous mCherry expression.

Depositor

Darrell Kotton

Publication

McCauley et al Cell Stem Cell. 2017 Jun 1;20(6):844-857

Insert

mCherry

Use

Lentiviral

Promoter

Ef1aL

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO {ChETA-mRuby2}on-W3SL

Plasmid

#111389

Purpose

AAV vector with hSynapsin promoter, Cre-ON ChETA-mRuby2 (for optogenetic activation), and W3SL regulatory cassette (for maximize cloning capacity)

Depositor

Adam Kepecs

Publication

Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

Inserts

ChETA-mRuby2
W3SL

Use

AAV

Tags

mRuby2

Expression

Mammalian

Mutation

W3SL is built from a shortened WPRE, an upstream …

Promoter

hSynapsin

Available since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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