We narrowed to 21,797 results for: Sis

1. pFLN

   Plasmid

   #40029

   Depositor

   Liqun Luo

   Article

   Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

   Inserts

   FRT-loxP
   Neo
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   None and SV40

   Available since

   Oct. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSBBi-RP CCS1(Δ304-316)

   Plasmid

   #139163

   Purpose

   SB-transposon with constitutive bi-directional promoter. One side contains coffee caffeine synthase 1 (CCS1) CCS-CTS deletion mutant; the other side contains RFP and puromycin resistance genes.

   Depositor

   Neal Devaraj

   Insert

   caffeine synthase 1 [Coffea arabica] CCS-CTS region deletion
   

   Use

   Transposon

   Tags

   Expression

   Mammalian

   Mutation

   deleted amino acids 304-319

   Promoter

   EF1α

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti-dCas9-KRAB-blast

   Plasmid

   #89567

   Purpose

   Plasmid expression dCas9 protein in fusion with KRAB domain

   Depositor

   Gary Hon

   Article

   Xie et al Mol Cell. 2017 Apr 20;66(2):285-299.e5.

   Insert

   dCas9
   

   Use

   Lentiviral

   Tags

   Blasticidin resistance gene and KRAB

   Expression

   Mutation

   D10A and H840A

   Promoter

   Available since

   June 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEcgRNA

   Plasmid

   #166581

   Purpose

   CRISPR-Cas9-assisted genome editing in Escherichia coli

   Depositor

   Sheng Yang

   Article

   Li et al Acta Biochim Biophys Sin (Shanghai). 202

   Insert

   ccdB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC_pThrRS_SeptRNAv2.0CUA_OXB20-PduX_EFSep

   Plasmid

   #173899

   Purpose

   The pThr/tRNAv2.0CUA pair enables the biosynthesis of proteins containing pThr

   Depositor

   Jason W Chin

   Article

   Zhang et al Nat Methods. 2017 Jul;14(7):729-736. doi

   Inserts

   ThrRS(v1.0)
   tRNA(v2.0)CUA
   pduX
   EF-Sep
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Gal4-VP16

   Plasmid

   #71728

   Purpose

   plasmid expressing fusion construct of Gal4 DBD and VP16 AD

   Depositor

   Lea Sistonen

   Article

   Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

   Insert

   Gal4 DBD(1-147) (GAL4 Budding Yeast)
   

   Use

   Tags

   VP16 AD (413-490)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBAM1

   Plasmid

   #60487

   Purpose

   This plasmids is a modular mini-Tn5 vector that could be used to generate random mutagenesis libraries or to insert heterologous DNA into a target genome

   Depositor

   Víctor de Lorenzo

   Article

   Martinez-Garcia et al BMC Microbiol. 2011 Feb 22;11:38. doi: 1

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJB06

   Plasmid

   #190480

   Purpose

   Base Cas9 expression plasmid for 2-plasmid C. difficile mutagenesis system

   Depositor

   Joseph Sorg

   Article

   Brehm et al Microbiol Resour Announc. 2022 Dec 15;11

   Inserts

   Cas9
   XylR
   

   Use

   E. coli / b. subtilis - c. difficile shuttle vect…

   Tags

   Expression

   Mutation

   Promoter

   xylR-driven promoter

   Available since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJB07

   Plasmid

   #190481

   Purpose

   Targeting plasmid for 2-plasmid C. difficile mutagenesis system

   Depositor

   Joseph Sorg

   Article

   Brehm et al Microbiol Resour Announc. 2022 Dec 15;11

   Inserts

   Upstream & Downstream pyrE deletion region
   gRNA
   

   Use

   E. coli / b. subtilis - c. difficile shuttle vect…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUV15-pHGFP

    Plasmid

    #70045

    Purpose

    encodes a pH-sensitive ratiometric GFP (pH-GFP), which allows for noninvasive measurements of intrabacterial pH in live cells

    Depositor

    Sabine Ehrt

    Article

    Vandal et al Nat Med. 2008 Aug;14(8):849-54. doi: 10.

    Insert

    phGFP
    

    Use

    Mycobacterium tuberculosis expression vector

    Tags

    Expression

    Mutation

    Promoter

    mycobacterial promoter Psmyc

    Available since

    Oct. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJYS2_crtYf

    Plasmid

    #85544

    Purpose

    Constitutive transcription of crRNA of crtYf, Spectinomycin resistance

    Depositor

    Sheng Yang

    Article

    Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

    Insert

    crRNA of crtYf
    

    Use

    CRISPR; Shuttle vector corynebacterium glutamicum…

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKIR1.0

    Plasmid

    #86409

    Purpose

    CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.

    Depositor

    Tetsuya Higashiyama

    Article

    Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

    Insert

    human-codon-optimized SpCas9
    

    Use

    CRISPR

    Tags

    FLAG and NLS

    Expression

    Mutation

    Promoter

    AtRPS5A

    Available since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBS-TarHom-Tol2-kanaR-cryaa-CFP

    Plasmid

    #74151

    Purpose

    BAC targeting cassette for pTARBAC2 with kanaR selection marker and lens-specific transgenesis marker Cerulean

    Depositor

    Holger Knaut

    Article

    Fuentes et al G3 (Bethesda). 2016 Jan 27. pii: g3.115.

    Insert

    Tol2-kanaR-cryaa-CFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHCMV_ALV_Env

    Plasmid

    #207325

    Purpose

    Expression of env polyprotein from Avian leukosis virus (ALV)

    Depositor

    Feng Zhang

    Article

    Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

    Insert

    ALV_Env (env Avian leukosis virus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mammalian codon optimization

    Promoter

    CMV promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLminP_Luc2P_RE28

    Plasmid

    #90370

    Purpose

    TFEB - Lysosomal biogenesis gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TFEB

    Available since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMi(3xP3-EGFP)

    Plasmid

    #102540

    Purpose

    Transgenesis vector based on the Minos transposon, carrying the 3xP3-EGFP marker

    Depositor

    Michalis Averof, Charalambos Savakis

    Article

    Pavlopoulos et al Genetics. 2004 Jun;167(2):737-46.

    Insert

    Minos transpoable element with 3xP3-EGFP marker
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. p5E-CMVSP6

    Plasmid

    #138356

    Purpose

    Gateway compatible 5' entry clone contains Cytomegalovirus and SP6 dual promoter(CMVSSP6)

    Depositor

    Nathan Lawson

    Article

    Sissaoui et al Circ Res. 2020 Feb 17. doi: 10.1161/CIRC

    Insert

    CMV-SP6 promoter
    

    Use

    Gateway compatible

    Tags

    Expression

    Mutation

    Promoter

    CMV-SP6

    Available since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLminP_Luc2P_RE21

    Plasmid

    #90363

    Purpose

    p53 - Apoptosis gene reporter

    Depositor

    Ramnik Xavier

    Article

    O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

    Insert

    Firefly luciferase
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    p53

    Available since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRSETa_ mEos4b

    Plasmid

    #51073

    Purpose

    Photoconverible Fluorescent Protein_ Fixative Resistant (monomeric)

    Depositor

    Loren Looger

    Article

    Paez-Segala et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Insert

    mEos4b
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET24a isPETase

    Plasmid

    #225759

    Purpose

    Expresses codon-optimized PETase from Ideonella sakaiensis in E. coli without native signal sequence and with a C-terminal his-tag

    Depositor

    Jason Boock

    Article

    Carter et al Microb Cell Fact. 2024 Nov 25;23(1):319.

    Insert

    isPETase
    

    Use

    Tags

    6xHis Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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