We narrowed to 11,993 results for: pcdna

1. pcDNA3.1(+)_Granulin 2+linker3

   Plasmid

   #176920

   Purpose

   express Granulin 2 with linker 3 in mammalian cells

   Depositor

   Thomas Kukar

   Article

   Holler et al eNeuro. 2017 Aug 18;4(4). pii: eN-NWR-01

   Insert

   Granulin 2+linker3 (GRN Human)
   

   Use

   Tags

   Twin-Strep and FLAG tags encoded after Signal Pep…

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-Lyn-sapphireAKAR

   Plasmid

   #118466

   Purpose

   cp-T-sapphire-based single-color PKA biosensor targeted to the plasma membrane.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   Lyn-sapphireAKAR
   

   Use

   Tags

   N-terminal targeting sequence from Lyn kinase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. HA-USPL1-C236A-pcDNA3.1

   Plasmid

   #85764

   Purpose

   mammalian expression of full length HA tagged USPL1, catalytic inactive variant C236A

   Depositor

   Frauke Melchior

   Article

   Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

   Insert

   USPL1, full length, catalytic inactive variant variant C236A (USPL1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   catalytic inactive mutant C236A

   Promoter

   CMV

   Available Since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. HA-USPL1-C236S-pcDNA3.1

   Plasmid

   #85763

   Purpose

   mammalian expression of full length HA tagged USPL1, catalytic inactive variant C236S

   Depositor

   Frauke Melchior

   Article

   Schulz et al EMBO Rep. 2012 Oct;13(10):930-8. doi: 10

   Insert

   USPL1, full length, catalytic inactive variant variant C236S (USPL1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   catalytic inactive mutant C236S

   Promoter

   CMV

   Available Since

   Jan. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1+_LN-HA-AGO2-WT

   Plasmid

   #92009

   Purpose

   Expresses Lambda-N-HA-AGO2 (WT)

   Depositor

   Joshua Mendell

   Article

   Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

   Insert

   AGO2 (AGO2 Human)
   

   Use

   Tags

   Lambda-N-HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pY117 (pcDNA3.1-huMb3Cpf1)

   Plasmid

   #92293

   Purpose

   Expression of humanized Mb3Cpf1

   Depositor

   Feng Zhang

   Insert

   huMb3Cpf1
   

   Use

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5FRT/TO-p97wt-mycStrep

   Plasmid

   #31837

   Depositor

   Hemmo Meyer

   Article

   Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

   Insert

   p97
   

   Use

   Tags

   Strep and myc

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA3.1(+)Gαi1-91-NLuc

   Plasmid

   #207148

   Purpose

   Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI1 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

   Depositor

   Tom Van Loy, Dominique Schols

   Article

   Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

   Insert

   GNAI1 (GNAI1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NLuc inserted at position L(91)K

   Promoter

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1(+)-TTF-1-HDD

   Plasmid

   #52063

   Purpose

   It encodes a mutant of TTF-1 in which the homeodomain is deleted

   Depositor

   David Mu

   Article

   Runkle et al J Biol Chem. 2012 Aug 17;287(34):28790-8

   Insert

   Thyroid Transcription Factor 1 (NKX2-1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Homeodomain deleted

   Promoter

   Available Since

   April 11, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3.1-CHRFAM7A-mGFP

    Plasmid

    #62633

    Purpose

    mammalian expression of human duplicated Alpha7 (CHRFAM7A) with mEGFP fused into M3-M4 loop

    Depositor

    Henry Lester

    Article

    Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

    Insert

    CHRFAM7A (CHRFAM7A Human)
    

    Use

    Tags

    mEGFP inserted in M3-M4 loop

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5/FRT/TO DNAJB6b

    Plasmid

    #19474

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DNAJB6b (DNAJB6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA5-Adrm1-Flag

    Plasmid

    #19418

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Yao et al Nat Cell Biol. 2006 Sep . 8(9):994-1002.

    Insert

    adhesion regulating molecule 1 (ADRM1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA5-FRT-TO-mCherry-N

    Plasmid

    #210271

    Purpose

    N-terminal mCherry-tagging of inserts for inducible expression. Use with Flp-In T-Rex system.

    Depositor

    Niels Mailand

    Article

    Hertz et al Nat Struct Mol Biol. 2023 Jul 20. doi: 1

    Type

    Empty backbone

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNA4.TO-ORF39-2xCSTREP

    Plasmid

    #136200

    Purpose

    Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF39

    Depositor

    Britt Glaunsinger

    Article

    Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

    Insert

    ORF39
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1-ROR1-V5/HIS

    Plasmid

    #205607

    Purpose

    Expression of human ROR1 receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    ROR1 receptor tyrosine kinase (ROR1 Human)
    

    Use

    Tags

    V5-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA-FRT-FAK0

    Plasmid

    #45047

    Purpose

    FAK fret sensor without helix

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

    Insert

    Focal Adhesion Kinase
    

    Use

    Tags

    FLAG, mCerulean, and mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-CARMA2sh-E138A

    Plasmid

    #97068

    Purpose

    Expresses Psoriasis-Linked mutant CARD14/CARMA2 in mammalian cells

    Depositor

    Pasquale Vito

    Article

    Scudiero et al Cell Death Dis. 2017 Feb 23;8(2):e2627.

    Insert

    CARD14 isoform 3 (CARD14 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    changed Glutamic Acid 138 to Alanine

    Promoter

    CMV

    Available Since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA3 Flag MKK4beta

    Plasmid

    #15517

    Depositor

    Roger Davis

    Article

    Derijard et al Science. 1995 Feb 3. 267(5198):682-5.

    Insert

    pCDNA3 Flag MKK4beta (MAP2K4 Human)
    

    Use

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 24, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3-HA-RIPK3 4KR

    Plasmid

    #78813

    Purpose

    To overexpress RIPK3 4KR in Mammalian Cell

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK3 (RIPK3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K55R, K89R, K363R, K501R

    Promoter

    CMV

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA-Flightless-1-LRR-HA

    Plasmid

    #21151

    Depositor

    Junying Yuan

    Article

    Li et al J Cell Biol. 2008 Apr 21. 181(2):321-33.

    Insert

    pcDNA3-Fli-Lrr-HA
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only