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pFH77

Plasmid

#125816

Purpose

Level0 enCas9-PolI3M-TBD, wheat codon optimized

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

enCas9-PolI3M-TBD, wheat codon optimized

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFH91

Plasmid

#125819

Purpose

Level0 nScCas9 (nickase), wheat codon optimized

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

nScCas9 (nickase), wheat codon optimized

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pOZ-FH-C Bach1 S990A

Plasmid

#73647

Purpose

Retroviral expression of Bach1 S990A mutant (BRCA binding mutant).

Depositor

Sharon Cantor

Insert

Bach1 (BACH1 Human)

Use

Retroviral

Tags

FLAG and His

Expression

Mammalian

Mutation

S990A (BRCA binding mutant)

Promoter

unknown

Available since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1 Bach1 K141A/K142A

Plasmid

#73642

Purpose

Mammalian expression of Bach1 K141A/K142A mutant (mlh1 binding mutant)

Depositor

Sharon Cantor

Insert

Bach1 (BACH1 Human)

Use

Tags

His and Myc

Expression

Mammalian

Mutation

K141A/K142A ( mlh1 binding mutant)

Promoter

CMV

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only
35S-frGC155-MXMT-NosT

Plasmid

#80172

Purpose

BiFC vector for transient expression of frGC155-MXMT in plants

Depositor

Yutaka Kodama

Article

Fujii et al Plant Biotech. 2015; 32(1): 81-87.

Insert

frGFP(156-238)

Use

Tags

frGC155

Expression

Plant

Mutation

Promoter

CaMV 35S promoter

Available since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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35S-psGC155-MXMT-NosT

Plasmid

#80171

Purpose

BiFC vector for transient expression of psGC155-MXMT in plants

Depositor

Yutaka Kodama

Article

Fujii et al Plant Biotech. 2015; 32(1): 81-87.

Insert

psGFP(156-238)

Use

Tags

psGC155

Expression

Plant

Mutation

Promoter

CaMV 35S promoter

Available since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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35S-sfGC155-MXMT-NosT

Plasmid

#80166

Purpose

BiFC vector for transient expression of sfGC155-MXMT in plants

Depositor

Yutaka Kodama

Article

Fujii et al Plant Biotech. 2015; 32(1): 81-87.

Insert

sfGFP(156-238)

Use

Tags

sfGC155

Expression

Plant

Mutation

Promoter

CaMV 35S promoter

Available since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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35S-psGN155-MXMT-NosT

Plasmid

#80170

Purpose

BiFC vector for transient expression of psGN155-MXMT in plants

Depositor

Yutaka Kodama

Article

Fujii et al Plant Biotech. 2015; 32(1): 81-87.

Insert

psGFP(1-155)

Use

Tags

MXMT and psGN155

Expression

Plant

Mutation

Promoter

CaMV 35S promoter

Available since

Aug. 22, 2016

Availability

Academic Institutions and Nonprofits only

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35S-sfGN155-MXMT-NosT

Plasmid

#80165

Purpose

BiFC vector for transient expression of sfGN155-MXMT in plants

Depositor

Yutaka Kodama

Article

Fujii et al Plant Biotech. 2015; 32(1): 81-87.

Insert

sfGFP(1-155)

Use

Tags

sfGN155

Expression

Plant

Mutation

Promoter

CaMV 35S promoter

Available since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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35S-frGN155-MXMT-NosT

Plasmid

#80164

Purpose

BiFC vector for transient expression of frGN155-MXMT in plants.

Depositor

Yutaka Kodama

Article

Fujii et al Plant Biotech. 2015; 32(1): 81-87.

Insert

frGFP(1-155)

Use

Tags

frGN155

Expression

Plant

Mutation

Promoter

CaMV 35S promoter

Available since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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35S-eGN155-MXMT-NosT

Plasmid

#80130

Purpose

BiFC vector for transient expression of eGN155-MXMT in plants

Depositor

Yutaka Kodama

Article

Fujii et al Plant Biotech. 2015; 32(1): 81-87.

Insert

eGFP(1-155)

Use

Tags

eGN155

Expression

Plant

Mutation

Promoter

CaMV 35S promoter

Available since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pYL322d1/N-Vn

Plasmid

#183153

Purpose

Bimolecular fluorescence complementation (BiFC) vector for transient expression of N-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at N terminus of Vn.

Depositor

Qinlong Zhu, Yao-Guang Liu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pYL322d1/Vn-C

Plasmid

#183154

Purpose

Bimolecular fluorescence complementation (BiFC) vector for transient expression of N-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at C terminus of Vn.

Depositor

Qinlong Zhu, Yao-Guang Liu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pGFPGUSPlus

Plasmid

#64401

Purpose

binary plant vector expressing GFP and GUS, both driven by a 35S promoter

Depositor

Claudia Vickers

Article

Vickers et al Biotechnol Lett. 2007 Nov;29(11):1793-6.

Inserts

GFP
GUSPlus

Use

Binary vector for plant transformation

Tags

His

Expression

Plant

Mutation

S65T

Promoter

35S

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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pDGB1_alpha1

Plasmid

#68224

Purpose

GoldenBraid level 1 Alpha plant expression vector.

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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PCR4-Hsp68::mCherry-SI-Hsp68::eGFP-H11

Plasmid

#211942

Purpose

dual-enSERT-2.2 vector for site-specific integration of.a two-color enhancer-reporter construct into the H11 locus (separated by a synthetic insulator)

Depositor

Evgeny Kvon

Article

Hollingsworth et al bioRxiv 2023.12.10.570890

Type

Empty backbone

Use

CRISPR and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

HSP68

Available since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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pUC19/N-mChe

Plasmid

#183159

Purpose

Plant transient expression vector containing mCherry tag. Unique nucleotide sequences Uc and Ud for cloning at N terminus of mCherry.

Depositor

Qinlong Zhu, Yao-Guang Liu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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P16ΔS:S10-(MCS)

Plasmid

#108182

Purpose

Binary vectors used for the constitutive expression of tripartite split-sfGFP components in plants

Depositor

Tzu-Yin Liu

Article

Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

P16ΔS

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pYPQ167

Plasmid

#69309

Purpose

Gateway entry vector with Cas9p

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

Cas9p (Plant codon-optimized; high GC content at 5 prime region)

Use

CRISPR

Tags

Expression

Mutation

NLS fusion

Promoter

Available since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pHEFI-OptoCARCD19-mScarlet

Plasmid

#170465

Purpose

Expresses an optically-controllable Chimeric antigen receptor that binds CD19 antigen, fused to mScarlet fluorophore

Depositor

John James

Article

Harris et al Mol Syst Biol. 2021 May;17(5):e10091. do

Insert

OptoCAR-CD19

Use

Lentiviral

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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