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pMDC32B-AtmiR173aTS-B/c

Plasmid

#227965

Purpose

Expression plasmid with a ccdB cassette flanked with two inverted BsaI restriction sites. For expressing syn-tasiRNAs in Arabidopsis thaliana.

Depositor

Alberto Carbonell

Article

Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Inserts

B/c
AtmiR173aTS

Expression

Plant

Promoter

35S and None

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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pENTR-NbmiR482aTS-B/c

Plasmid

#227966

Purpose

Entry plasmid with NbmiR482aTS and a ccdB cassette flanked with two inverted BsaI restriction sites. Allows the high throughput cloning of cloning inserts flanked with compatible 4-nt overhangs.

Depositor

Alberto Carbonell

Article

Cisneros et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Inserts

B/c
NbmiR482aTS

Promoter

None

Available Since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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pMT1-13X-NOG(AtUBI10)-MTAP-LUC

Plasmid

#234371

Purpose

T-DNA vector for expression of the the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAs, no gRNA included (NOG)

Depositor

Michael Smanski

Article

Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70

Inserts

NbGP41-sfGFP-VP64-GB1
Luciferase
dCas9-13XGP41

Use

Synthetic Biology

Tags

GB1, GP41, and sfGFP

Expression

Plant

Promoter

Arabidopsis Ubi10 and MTAP1 (synthetic)

Available Since

April 17, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-FOXA1-T2A-miRFP670

Plasmid

#182335

Purpose

Expresses human FOXA1 and miRFP670 via T2A linker under control of a CMV promoter.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Insert

Forkhead Box A1 (FOXA1 Human)

Tags

T2A-miRFP670

Expression

Mammalian

Promoter

CMV

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pRAR064

Plasmid

#185031

Purpose

400 nt l31 promoter region, scrambled 5'UTR, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

Depositor

Julius Lucks

Article

Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

Insert

L31-sfGFP

Expression

Bacterial

Mutation

Nucleotides of rpmE 5'UTR scrambled, Only fi…

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pRAR102

Plasmid

#185033

Purpose

400 nt l31 promoter region, l31 5'UTR U54C mutation, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

Depositor

Julius Lucks

Article

Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

Insert

L31-sfGFP

Expression

Bacterial

Mutation

U54C mutation of rpmE 5'UTR, Only first 90 n…

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pRAR106

Plasmid

#185036

Purpose

400 nt l31 promoter region, l31 5'UTR G79U mutation, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

Depositor

Julius Lucks

Article

Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

Insert

L31-sfGFP

Expression

Bacterial

Mutation

G79U mutation of rpmE 5'UTR, Only first 90 n…

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pRAR116

Plasmid

#185040

Purpose

400 nt l31 promoter region, l31 5'UTR U58C and U70C, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

Depositor

Julius Lucks

Article

Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

Insert

L31-sfGFP

Expression

Bacterial

Mutation

U58C and U70C of rpmE of 5'UTR, Only first 9…

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pRAR017-L31-sfGFP

Plasmid

#185024

Purpose

400 nt l31 promoter region, l31 5'UTR, 90 nt of l31 coding region, glycine linker, sfGFP, trrnB

Depositor

Julius Lucks

Article

Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

Insert

L31-sfGFP

Expression

Bacterial

Mutation

Only first 90 nt of rpmE coding present

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1-Cdc45-6His

Plasmid

#211379

Purpose

bacterial expression of human Cdc45 C-terminally fused to 6xHis-Tag

Depositor

Helmut Pospiech

Article

Szambowska et al Nucleic Acids Res. 2014 Feb;42(4):2308-1

Insert

Cdc45 (CDC45 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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Halo-S13S2DBD

Plasmid

#200196

Purpose

Expression of Sox13 with Sox2 DBD

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

Halo-S13S2DBD (Sox2, Sox13 Mouse)

Tags

Halo

Expression

Mammalian

Mutation

Sox13(1-1242); Sox 2 (126-333); Sox13 (1449-1839)

Promoter

EF_1alpha

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pUC57-RPLP15UTR-hRluc-A60

Plasmid

#182639

Purpose

Transcription template plasmid for RPLP1 5'UTR followed by Renilla luciferase open reading frame and polyA sequence

Depositor

Stephen Floor

Article

Calviello et al Nucleic Acids Res. 2021 May 21;49(9):533

Insert

Renilla luciferase

Use

Luciferase

Expression

Mammalian

Mutation

The transcription is driven by a T7 promoter, pai…

Available Since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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Halo-S2S13DBD

Plasmid

#200195

Purpose

Expression of Sox2 with Sox13 DBD

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

Halo-S2S13DBD (Sox2, Sox13 Mouse)

Tags

Halo

Expression

Mammalian

Mutation

Sox2(1-126); Sox13 (1242-1449); Sox2 (333-957)

Promoter

EF_1alpha

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsDCP1a-H19A_V

Plasmid

#147799

Purpose

Mammalian Expression of HsDCP1a-H19A

Depositor

Elisa Izaurralde

Article

Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

Insert

HsDCP1a-H19A (DCP1A Human)

Expression

Mammalian

Mutation

two silent mutations T237C and T1716G compared to…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsDCP1a-N74G_V

Plasmid

#147800

Purpose

Mammalian Expression of HsDCP1a-N74G

Depositor

Elisa Izaurralde

Article

Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

Insert

HsDCP1a-N74G (DCP1A Human)

Expression

Mammalian

Mutation

two silent mutations T237C and T1716G compared to…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsDCP1a-Q18AH19A_V

Plasmid

#147801

Purpose

Mammalian Expression of HsDCP1a-Q18AH19A

Depositor

Elisa Izaurralde

Article

Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

Insert

HsDCP1a-Q18AH19A (DCP1A Human)

Expression

Mammalian

Mutation

two silent mutations T237C and T1716G compared to…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsDCP1a-R72G_V

Plasmid

#147802

Purpose

Mammalian Expression of HsDCP1a-R72G

Depositor

Elisa Izaurralde

Article

Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

Insert

HsDCP1a-R72G (DCP1A Human)

Expression

Mammalian

Mutation

two silent mutations T237C and T1716G compared to…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsDCP1a-R72GN74G_V

Plasmid

#147803

Purpose

Mammalian Expression of HsDCP1a-R72GN74G

Depositor

Elisa Izaurralde

Article

Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

Insert

HsDCP1a-R72GN74G (DCP1A Human)

Expression

Mammalian

Mutation

two silent mutations T237C and T1716G compared to…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsDCP2_1-244_V

Plasmid

#147790

Purpose

Mammalian Expression of HsDCP2_1-244

Depositor

Elisa Izaurralde

Article

Chang et al Nucleic Acids Res. 2014 Apr;42(8):5217-3

Insert

HsDCP2_1-244 (DCP2 Human)

Expression

Mammalian

Mutation

one silent mutation A429G compared to the sequenc…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-CeAIN2_254-400-HsTNRC6C_1207-1690-CIM1mut_R

Plasmid

#147395

Purpose

Insect Expression of CeAIN2_254-400-HsTNRC6C_1207-1690-CIM1mut

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

CeAIN2_254-400-HsTNRC6C_1207-1690-CIM1mut (TNRC6C Human, Nematode)

Expression

Insect

Mutation

Deletion of 2 AA (SD) at position 378-379 compare…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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