We narrowed to 4,780 results for: Drosophila

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pSEED-OLLAS:HA

Plasmid

#219647

Purpose

Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in OLLAS and HA tags using the SEED/Harvest method

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

OLLAS:HA SEED Cassette

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pR6Kg-Alpha1

Plasmid

#165862

Purpose

Alpha level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived) uses blue-white screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Type

Empty backbone

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pJFRC7-UAS-NPRR_ANP

Plasmid

#133243

Purpose

Fluorescent reporter for neuropeptide release imaging

Depositor

David Anderson

Article

Ding et al Elife. 2019 Jun 26;8. pii: 46421. doi: 1

Insert

rat atrial natriuretic peptide-fused codon-optimized GCaMP6s

Expression

Insect

Promoter

hsp70

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMycLAM

Plasmid

#71811

Purpose

Generating transgenic flies for FLP-inducible DamID

Depositor

Bas van Steensel

Article

Pindyurin et al Nucleic Acids Res. 2016 Jul 8;44(12):564

Insert

min.hsp70P-FRT-STOP#1-FRT-Dam-Myc-LAM

Expression

Insect

Available Since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

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pET16b-Antp

Plasmid

#86866

Purpose

Bacterial expression of fusion protein with cell penetrating peptide, antennapedia fragment, at C-terminus.

Depositor

Michael Chin

Article

Chien et al Genesis. 2014 Jul;52(7):695-701. doi: 10

Insert

Linker-Antp

Tags

Antp

Expression

Bacterial

Available Since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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pR6Kg-Omega1

Plasmid

#165864

Purpose

Omega level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived)

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Type

Empty backbone

Use

Synthetic Biology

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pQUAST-syb-spCFP1-10

Plasmid

#73699

Purpose

Neuronal-synaptobrevin (syb):spCFP1–10 chimera for synaptic GRASP

Depositor

Marco Gallio

Article

Macpherson et al Nat Commun. 2015 Dec 4;6:10024. doi: 10.

Insert

Neuronal-synaptobrevin:spCFP1–10 chimera

Expression

Insect

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pHRW-CRY2olig-VHH(GFP)

Plasmid

#126023

Purpose

express RFP-tagged CRY2 PHR E490G fused to anti-GFP nanobody for optogenetics

Depositor

Eurico Morais-de-Sá

Article

Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: bio

Insert

CRY2 PHRolig-VHH(GFP)

Tags

RFP

Expression

Insect

Mutation

CRY2 E490G

Promoter

Hsp70

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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OA-1050G (white)

Plasmid

#132420

Purpose

express arrays of gRNA targeting White under dU6-3 promoter

Depositor

Omar Akbari

Article

Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

Insert

white gRNA array

Expression

Insect

Promoter

U6-3

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pB1H2w2-12-C81S

Plasmid

#18042

Depositor

Scot Wolfe

Article

Noyes et al Nucleic Acids Res. 2008 May;36(8):2547-6

Insert

HhaI C81S mutant

Expression

Bacterial

Mutation

Simply used as an insert to drop out between Kpn1…

Available Since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

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907H1

Plasmid

#160234

Purpose

FlightN-CymR-VP16-3X-SV40-attB

Depositor

Omar Akbari

Article

Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

Inserts

p-cymene repressor
Flightin promoter

Tags

VP16

Expression

Insect

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pB1H2w2-12-Oct1

Plasmid

#18041

Depositor

Scot Wolfe

Article

Noyes et al Nucleic Acids Res. 2008 May;36(8):2547-6

Insert

HD of Oct1

Expression

Bacterial

Mutation

the homeodomain of Oct1 is fused to fingers 1 and…

Available Since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 myc his Su(fu) (CT#768)

Plasmid

#13855

Depositor

Cliff Tabin

Article

Pearse et al Dev Biol. 1999 Aug 15. 212(2):323-36.

Insert

Su(fu) (SUFU Chicken)

Tags

his and myc

Expression

Mammalian

Mutation

full ORF

Available Since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pF3BGX-T2A-GAL4

Plasmid

#138392

Purpose

RMCE vector for generating C-terminal T2A-GAL4 fusion

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

T2A-GAL4

Expression

Insect

Available Since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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pAc5 dOrai

Plasmid

#16368

Depositor

Anjana Rao

Article

Gwack et al J Biol Chem. 2007 Jun 1. 282(22):16232-4

Insert

Orai (olf186-F Fly)

Tags

V5

Expression

Insect

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
pSK ZO1

Plasmid

#30316

Depositor

James Anderson, Alan Fanning

Article

Willott et al Proc Natl Acad Sci U S A. 1993 Aug 15. 9

Insert

Zonula Occludens-1 (TJP1 Human)

Use

Subcloning

Available Since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only
pHD-mW-attPCC-FRT (pTL717)

Plasmid

#115158

Purpose

Vector for generating dsDNA donors for HDR. Contains an attP-CC docking site, the visible GMR-white for identifying backbone incorporation flanked by FRT sites for FLP-mediated removal.

Depositor

Tobias Langenhan

Article

Blanco-Redondo et al G3 (Bethesda). 2018 Jul 31. pii: g3.118.

Insert

mW attP-CC FRT

Expression

Insect

Available Since

Oct. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pHygroR-GMRWhite

Plasmid

#165886

Purpose

Hygromycin resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screening

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Transgenesis Ready Selection Vector

Use

Synthetic Biology

Available Since

April 2, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac FPSC

Plasmid

#1926

Depositor

Robert Kingston

Article

Francis et al Mol Cell 2001 Sep;8(3):545-56.

Insert

posterior sex combs (Psc Fly)

Tags

flag

Expression

Insect

Available Since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only
scribble cDNA

Plasmid

#42064

Depositor

Norbert Perrimon

Article

Bilder et al Nature. 2000 Feb 10;403(6770):676-80.

Insert

scribble (scrib Fly)

Available Since

Feb. 20, 2013

Availability

Academic Institutions and Nonprofits only